Restriction map for
pF1KB3061
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbvCI BcgI BglI BlnI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspMI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CfoI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
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AcsI 725 GAATTC [GA]^AATT[CT]
AflII 522 CTTAAG C^TTAAG
AlwNI 259 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1476 CCTGAGG CC^T[ACGT]AGG
ApoI 725 GAATTC [GA]^AATT[CT]
AseI 396 ATTAAT AT^TAAT
AsnI 396 ATTAAT AT^TAAT
BalI 1626 TGGCCA TGG^CCA
BbrPI 1086 CACGTG CAC^GTG
BbsI 1188 GAAGAC GAAGAC(2/6)
BciVI 641 GTATCC GTATCC(6/5)
BclI 447 TGATCA T^GATCA
BfaI 563 CTAG C^TAG
BfrI 522 CTTAAG C^TTAAG
BglII 789 AGATCT A^GATCT
BlpI 796 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 796 GCTCAGC GC^T[ACGT]AGC
BpuAI 1188 GAAGAC GAAGAC(2/6)
BsaAI 1086 CACGTG [CT]AC^GT[GA]
BsaI 1376 GGTCTC GGTCTC(1/5)
BseRI 73 CTCCTC GAGGAG(10/8)
BsiEI 1116 CGACCG CG[GA][CT]^CG
Bsp1407I 313 TGTACA T^GTACA
BspLU11I 581 ACATGT A^CATGT
BsrDI 1007 GCAATG GCAATG(2/0)
BsrGI 313 TGTACA T^GTACA
BssSI 1222 CACGAG CACGAG(-5/-1)
BstAPI 1511 GCAATAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1476 CCTGAGG CC^T[ACGT]AGG
CelII 796 GCTCAGC GC^T[ACGT]AGC
DraIII 1141 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1117 GACCGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 340 CCATGG C^C[GA][CT]GG
Eco81I 1476 CCTGAGG CC^T[ACGT]AGG
EcoRI 725 GAATTC G^AATTC
EcoT22I 1253 ATGCAT ATGCA^T
FbaI 447 TGATCA T^GATCA
HincII 499 GTTGAC GT[CT]^[GA]AC
HindII 499 GTTGAC GT[CT]^[GA]AC
HindIII 474 AAGCTT A^AGCTT
MaeI 563 CTAG C^TAG
MaeIII 272 GTGAC ^GT[ACGT]AC
MluNI 1626 TGGCCA TGG^CCA
MscI 1626 TGGCCA TGG^CCA
NaeI 27 GCCGGC GCC^GGC
NcoI 340 CCATGG C^CATGG
NgoMIV 27 GCCGGC G^CCGGC
NsiI 1253 ATGCAT ATGCA^T
PmaCI 1086 CACGTG CAC^GTG
PmlI 1086 CACGTG CAC^GTG
PshAI 379 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 396 ATTAAT AT^TAAT
PstI 1048 CTGCAG CTGCA^G
PvuII 202 CAGCTG CAG^CTG
SapI 252 GAAGAGC GCTCTTC(1/4)
ScaI 1538 AGTACT AGT^ACT
SspBI 313 TGTACA T^GTACA
TaqI 56 TCGA T^CGA
Tsp45I 272 GTGAC ^GT[GC]AC
TthHB8I 56 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 581 ACATGT A^C[GA][CT]GT
1085 ACACGT
BanII 699 GAGCTC G[GA]GC[CT]^C
783 GAGCTC
BcnI 1070 CCGGG CC^[GC]GG
1154 CCGGG
BpmI 205 CTGGAG CTGGAG(16/14)
650 CTGGAG
BsmAI 160 GTCTC GTCTC(1/5)
1377 GTCTC
BsmFI 92 GGGAC GGGAC(10/14)
168 GGGAC
BsrFI 27 GCCGGC [GA]^CCGG[CT]
1373 GCCGGT
Cfr10I 27 GCCGGC [GA]^CCGG[CT]
1373 GCCGGT
EaeI 609 TGGCCG [CT]^GGCC[GA]
1626 TGGCCA
EarI 252 GAAGAG CTCTTC(1/4)
1035 GAAGAG
EcoNI 75 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
434 CCTTGGGGAGG
FauI 1520 GCGGG CCCGC(4/6)
1595 GCGGG
Ksp632I 252 GAAGAG CTCTTC(1/4)
1035 GAAGAG
MaeII 1087 ACGT A^CGT
1145 ACGT
MseI 397 TTAA T^TAA
523 TTAA
NciI 1070 CCGGG CC^[GC]GG
1154 CCGGG
NdeI 914 CATATG CA^TATG
1502 CATATG
SacI 699 GAGCTC GAGCT^C
783 GAGCTC
SfaNI 633 GCATC GCATC(5/9)
801 GCATC
SphI 533 GCATGC GCATG^C
1255 GCATGC
Tru9I 397 TTAA T^TAA
523 TTAA
Tsp509I 399 AATT ^AATT
726 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
ApaLI Asp700I AspHI AvaII BamHI
BbvI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StuI StyI TfiI TseI
TspRI XhoII XmnI