Restriction map for
pF1KB3089
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KasI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 619 GACGTC GACGT^C
AccI 616 GTAGAC GT^[AC][GT]AC
AcsI 118 AAATTT [GA]^AATT[CT]
AcyI 619 GACGTC G[GA]^CG[CT]C
AhdI 334 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 46 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 126 CCTAAGG CC^T[ACGT]AGG
Aor51HI 806 AGCGCT AGC^GCT
ApoI 118 AAATTT [GA]^AATT[CT]
AspEI 334 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 795 GTGCTC G[AT]GC[AT]^C
AspI 451 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 582 CCCGAG C^[CT]CG[GA]G
BbvCI 148 GCTGAGG CCTCAGC(-5/-2)
BciVI 469 GGATAC GTATCC(6/5)
BpmI 43 CTCCAG CTGGAG(16/14)
BsaHI 619 GACGTC G[GA]^CG[CT]C
BsgI 870 GTGCAG GTGCAG(16/14)
BsiHKAI 795 GTGCTC G[AT]GC[AT]^C
BsoBI 582 CCCGAG C^[CT]CG[GA]G
Bsp1407I 296 TGTACA T^GTACA
BspMI 1374 ACCTGC ACCTGC(4/8)
BsrDI 498 CATTGC GCAATG(2/0)
BsrGI 296 TGTACA T^GTACA
Bsu36I 126 CCTAAGG CC^T[ACGT]AGG
CfoI 807 GCGC GCG^C
DrdI 313 GACTCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 334 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 806 AGCGCT AGC^GCT
Eco81I 126 CCTAAGG CC^T[ACGT]AGG
HaeII 806 AGCGCT [GA]GCGC^[CT]
HhaI 807 GCGC GCG^C
Hin1I 619 GACGTC G[GA]^CG[CT]C
HinP1I 807 GCGC G^CGC
MaeII 620 ACGT A^CGT
MseI 437 TTAA T^TAA
MslI 324 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 237 CCATGG C^CATGG
NspI 684 GCATGT [GA]CATG^[CT]
PflFI 451 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 459 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 864 CTACAG C^T[GA][CT]AG
SmlI 906 CTTGAG C^T[CT][GA]AG
SspBI 296 TGTACA T^GTACA
Tru9I 437 TTAA T^TAA
Tsp45I 342 GTCAC ^GT[GC]AC
TspRI 365 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 451 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 459 CCATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 234 GATCC GGATC(4/5)
547 GATCC
BfaI 787 CTAG C^TAG
1337 CTAG
BseRI 427 GAGGAG GAGGAG(10/8)
430 GAGGAG
BssSI 802 CACGAG CACGAG(-5/-1)
19 CTCGTG
BstNI 649 CCTGG CC^[AT]GG
721 CCAGG
BstXI 383 CCAAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
486 CCATGCTGCTGG
Cac8I 373 GCGGGC GC[ACGT]^[ACGT]GC
493 GCTGGC
DraII 130 AGGACCC [GA]G^G[ACGT]CC[CT]
643 GGGTCCC
EcoO109I 130 AGGACCC [GA]G^G[ACGT]CC[CT]
643 GGGTCCC
EcoRII 649 CCTGG ^CC[AT]GG
721 CCAGG
FauI 62 CCCGC CCCGC(4/6)
373 GCGGG
HincII 526 GTCAAC GT[CT]^[GA]AC
688 GTTGAC
HindII 526 GTCAAC GT[CT]^[GA]AC
688 GTTGAC
KspI 63 CCGCGG CCGC^GG
371 CCGCGG
MaeI 787 CTAG C^TAG
1337 CTAG
MvaI 649 CCTGG CC^[AT]GG
721 CCAGG
PpuMI 130 AGGACCC [GA]G^G[AT]CC[CT]
643 GGGTCCC
PvuII 146 CAGCTG CAG^CTG
551 CAGCTG
SacII 63 CCGCGG CCGC^GG
371 CCGCGG
SfaNI 604 GATGC GCATC(5/9)
1005 GATGC
StuI 860 AGGCCT AGG^CCT
1079 AGGCCT
XcmI 317 CCAATGTCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
669 CCAGAAACCAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstUI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
StyI TaqI TfiI TseI Tsp509I
TthHB8I