Restriction map for
pF1KB3100
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI AspEI AspI AvrII BalI
BamHI BbeI BclI BfrI BglII
BlnI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KasI MflI MluI
MluNI MroI MscI MseI NarI
NdeI NheI NotI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tru9I
Tsp45I Tth111I XbaI XhoII
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1360 GGTACC G^GTACC
AccI 819 GTCTAC GT^[AC][GT]AC
AcyI 145 GACGCC G[GA]^CG[CT]C
AlwI 1440 GATCC GGATC(4/5)
AlwNI 824 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 321 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1360 GGTACC G^GTACC
AviII 1129 TGCGCA TGC^GCA
BbrPI 838 CACGTG CAC^GTG
BbsI 423 GAAGAC GAAGAC(2/6)
BcgI 402 GCAGGCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1223 GTATCC GTATCC(6/5)
BlpI 88 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 88 GCTGAGC GC^T[ACGT]AGC
BpuAI 423 GAAGAC GAAGAC(2/6)
BsaAI 838 CACGTG [CT]AC^GT[GA]
BsaBI 780 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 145 GACGCC G[GA]^CG[CT]C
BsmBI 1542 GAGACG CGTCTC(1/5)
BspMI 2343 ACCTGC ACCTGC(4/8)
BsrDI 1059 CATTGC GCAATG(2/0)
CelII 88 GCTGAGC GC^T[ACGT]AGC
EcoNI 1719 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1129 TGCGCA TGC^GCA
HgaI 145 GACGC GACGC(5/10)
Hin1I 145 GACGCC G[GA]^CG[CT]C
HindIII 470 AAGCTT A^AGCTT
KpnI 1360 GGTACC GGTAC^C
MamI 780 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 704 CAATTG C^AATTG
MslI 719 CATACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 704 CAATTG C^AATTG
NaeI 1403 GCCGGC GCC^GGC
NcoI 299 CCATGG C^CATGG
NgoMIV 1403 GCCGGC G^CCGGC
NruI 33 TCGCGA TCG^CGA
NspI 1320 ACATGC [GA]CATG^[CT]
PaeR7I 65 CTCGAG C^TCGAG
PflMI 1702 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 838 CACGTG CAC^GTG
PmlI 838 CACGTG CAC^GTG
SacI 139 GAGCTC GAGCT^C
SapI 889 GAAGAGC GCTCTTC(1/4)
ScaI 2474 AGTACT AGT^ACT
SfaNI 1383 GATGC GCATC(5/9)
SspI 307 AATATT AAT^ATT
Van91I 1702 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1364 CCAATGCCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 65 CTCGAG C^TCGAG
XmnI 321 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Aor51HI 1820 AGCGCT AGC^GCT
1880 AGCGCT
BanI 1231 GGCACC G^G[CT][GA]CC
1360 GGTACC
BbvCI 583 CCTCAGC CCTCAGC(-5/-2)
2425 CCTCAGC
BglI 237 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
486 GCCAGACAGGC
BpmI 214 CTGGAG CTGGAG(16/14)
1904 CTGGAG
BsrFI 941 GCCGGT [GA]^CCGG[CT]
1403 GCCGGC
Cfr10I 941 GCCGGT [GA]^CCGG[CT]
1403 GCCGGC
DraIII 1452 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2546 CACAGGGTG
EaeI 1422 TGGCCG [CT]^GGCC[GA]
2497 TGGCCG
EarI 598 GAAGAG CTCTTC(1/4)
889 GAAGAG
Eco47III 1820 AGCGCT AGC^GCT
1880 AGCGCT
EcoT14I 299 CCATGG C^C[AT][AT]GG
1085 CCTTGG
Ksp632I 598 GAAGAG CTCTTC(1/4)
889 GAAGAG
KspI 51 CCGCGG CCGC^GG
1857 CCGCGG
PpuMI 1607 AGGACCT [GA]G^G[AT]CC[CT]
1896 AGGACCC
SacII 51 CCGCGG CCGC^GG
1857 CCGCGG
StyI 299 CCATGG C^C[AT][AT]GG
1085 CCTTGG
TfiI 1070 GAATC G^A[AT]TC
1266 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SmaI SmlI StuI TaqI
TseI Tsp509I TspRI TthHB8I XmaCI
XmaI