Restriction map for
pF1KB3118
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BanI
BbeI BcgI BciVI BclI BcnI
BfrI BglI BglII BsaBI BsaHI
BsiEI BsiWI BsmBI BsoBI BspDI
BspHI BspMI BsrBI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MluI MluNI
MscI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 839 TCCGGA T^CCGGA
AflIII 2287 ACATGT A^C[GA][CT]GT
Alw44I 945 GTGCAC G^TGCAC
AocI 1549 CCTCAGG CC^T[ACGT]AGG
ApaLI 945 GTGCAC G^TGCAC
AseI 181 ATTAAT AT^TAAT
AsnI 181 ATTAAT AT^TAAT
AspHI 945 GTGCAC G[AT]GC[AT]^C
AvrII 1635 CCTAGG C^CTAGG
BamHI 1272 GGATCC G^GATCC
BanII 1585 GGGCTC G[GA]GC[CT]^C
BbrPI 2344 CACGTG CAC^GTG
BbvCI 2332 CCTCAGC CCTCAGC(-5/-2)
BlnI 1635 CCTAGG C^CTAGG
BlpI 213 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 213 GCTCAGC GC^T[ACGT]AGC
BsaAI 2344 CACGTG [CT]AC^GT[GA]
BsaWI 839 TCCGGA [AT]^CCGG[AT]
BseAI 839 TCCGGA T^CCGGA
BseRI 2279 CTCCTC GAGGAG(10/8)
BsiHKAI 945 GTGCAC G[AT]GC[AT]^C
BsmFI 1005 GTCCC GGGAC(10/14)
BsmI 1474 GAATGC GAATGC(1/-1)
BspEI 839 TCCGGA T^CCGGA
BspLU11I 2287 ACATGT A^CATGT
BsrDI 706 GCAATG GCAATG(2/0)
BsrFI 51 GCCGGT [GA]^CCGG[CT]
BssSI 948 CACGAG CACGAG(-5/-1)
BstEII 1576 GGTCACC G^GT[ACGT]ACC
BstPI 1576 GGTCACC G^GT[ACGT]ACC
BstXI 2241 CCAAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1272 GGATCC [GA]^GATC[CT]
Bsu36I 1549 CCTCAGG CC^T[ACGT]AGG
CelII 213 GCTCAGC GC^T[ACGT]AGC
CfoI 14 GCGC GCG^C
Cfr10I 51 GCCGGT [GA]^CCGG[CT]
Eco81I 1549 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1576 GGTCACC G^GT[ACGT]ACC
EcoRI 346 GAATTC G^AATTC
HaeII 13 AGCGCC [GA]GCGC^[CT]
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
HincII 1708 GTTGAC GT[CT]^[GA]AC
HindII 1708 GTTGAC GT[CT]^[GA]AC
MfeI 1986 CAATTG C^AATTG
MflI 1272 GGATCC [GA]^GATC[CT]
MroI 839 TCCGGA T^CCGGA
MunI 1986 CAATTG C^AATTG
NdeI 660 CATATG CA^TATG
NheI 151 GCTAGC G^CTAGC
PmaCI 2344 CACGTG CAC^GTG
PmlI 2344 CACGTG CAC^GTG
PshBI 181 ATTAAT AT^TAAT
SmlI 1824 CTCAAG C^T[CT][GA]AG
SpeI 1303 ACTAGT A^CTAGT
StuI 324 AGGCCT AGG^CCT
TaqI 2308 TCGA T^CGA
TthHB8I 2308 TCGA T^CGA
XbaI 1854 TCTAGA T^CTAGA
XhoII 1272 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwNI 358 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
551 CAGAGTCTG
BmyI 945 GTGCAC G[AGT]GC[ACT]^C
1585 GGGCTC
BsiYI 79 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1545 CCAGCCTCAGG
BslI 79 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1545 CCAGCCTCAGG
Bsp1286I 945 GTGCAC G[AGT]GC[ACT]^C
1585 GGGCTC
DraI 2394 TTTAAA TTT^AAA
2418 TTTAAA
HgaI 218 GCGTC GACGC(5/10)
513 GCGTC
MaeII 1747 ACGT A^CGT
2345 ACGT
MslI 463 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2340 CACCCACGTG
SfcI 300 CTGTAG C^T[GA][CT]AG
389 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BpmI BpuAI BsaI BsaJI BsgI
BsmAI Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshAI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI