################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpmI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AspHI 406 GAGCTC G[AT]GC[AT]^C AspI 190 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 563 GGATCC G^GATCC BanII 406 GAGCTC G[GA]GC[CT]^C BbsI 651 GAAGAC GAAGAC(2/6) BcgI 84 CGAGTGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 293 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 255 GCTCAGC GC^T[ACGT]AGC Bpu1102I 255 GCTCAGC GC^T[ACGT]AGC BpuAI 651 GAAGAC GAAGAC(2/6) BsiEI 194 CGGTCG CG[GA][CT]^CG BsiHKAI 406 GAGCTC G[AT]GC[AT]^C BsmAI 645 GTCTC GTCTC(1/5) BspMI 551 ACCTGC ACCTGC(4/8) BsrI 428 ACTGG ACTGG(1/-1) BstXI 392 CCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 563 GGATCC [GA]^GATC[CT] CelII 255 GCTCAGC GC^T[ACGT]AGC DpnII 564 GATC ^GATC EarI 409 CTCTTC CTCTTC(1/4) EcoNI 288 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 484 GTCAAC GT[CT]^[GA]AC HindII 484 GTCAAC GT[CT]^[GA]AC HinfI 654 GACTC G^A[ACGT]TC Ksp632I 409 CTCTTC CTCTTC(1/4) KspI 242 CCGCGG CCGC^GG MboI 564 GATC ^GATC MflI 563 GGATCC [GA]^GATC[CT] NaeI 298 GCCGGC GCC^GGC NdeII 564 GATC ^GATC NgoMIV 298 GCCGGC G^CCGGC NspI 614 GCATGC [GA]CATG^[CT] PflFI 190 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 654 GACTC GAGTC(4/5) PstI 214 CTGCAG CTGCA^G PvuII 352 CAGCTG CAG^CTG SacI 406 GAGCTC GAGCT^C SacII 242 CCGCGG CCGC^GG SapI 408 GCTCTTC GCTCTTC(1/4) Sau3AI 564 GATC ^GATC SfcI 214 CTGCAG C^T[GA][CT]AG SphI 614 GCATGC GCATG^C Tth111I 190 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 563 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 564 GATCC GGATC(4/5) 563 GGATC BbeI 106 GGCGCC GGCGC^C 235 GGCGCC BmyI 160 GGGCAC G[AGT]GC[ACT]^C 406 GAGCTC BseRI 147 CTCCTC GAGGAG(10/8) 403 GAGGAG BsgI 355 CTGCAC GTGCAG(16/14) 553 CTGCAC Bsp1286I 160 GGGCAC G[AGT]GC[ACT]^C 406 GAGCTC BsrFI 298 GCCGGC [GA]^CCGG[CT] 545 ACCGGC Cfr10I 298 GCCGGC [GA]^CCGG[CT] 545 ACCGGC DsaI 242 CCGCGG C^C[GA][CT]GG 392 CCACGG HaeII 106 GGCGCC [GA]GCGC^[CT] 235 GGCGCC KasI 106 GGCGCC G^GCGCC 235 GGCGCC MslI 393 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 468 CATGTGTGTG NarI 106 GGCGCC GG^CGCC 235 GGCGCC NlaIII 468 CATG CATG^ 615 CATG PpuMI 188 GGGACCC [GA]G^G[AT]CC[CT] 202 AGGACCT SfoI 106 GGCGCC GGC^GCC 235 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BbvI BcnI BsaHI BsaJI BsiYI BslI BsmFI BsoBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI SfaNI SmaI TseI XmaCI XmaI