Restriction map for pF1KB3129
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BpmI        
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AspHI         406  GAGCTC               G[AT]GC[AT]^C
AspI          190  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         563  GGATCC               G^GATCC
BanII         406  GAGCTC               G[GA]GC[CT]^C
BbsI          651  GAAGAC               GAAGAC(2/6)
BcgI           84  CGAGTGGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          293  GCCAGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          255  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      255  GCTCAGC              GC^T[ACGT]AGC
BpuAI         651  GAAGAC               GAAGAC(2/6)
BsiEI         194  CGGTCG               CG[GA][CT]^CG
BsiHKAI       406  GAGCTC               G[AT]GC[AT]^C
BsmAI         645  GTCTC                GTCTC(1/5)
BspMI         551  ACCTGC               ACCTGC(4/8)
BsrI          428  ACTGG                ACTGG(1/-1)
BstXI         392  CCACGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         563  GGATCC               [GA]^GATC[CT]
CelII         255  GCTCAGC              GC^T[ACGT]AGC
DpnII         564  GATC                 ^GATC
EarI          409  CTCTTC               CTCTTC(1/4)
EcoNI         288  CCTCCGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        484  GTCAAC               GT[CT]^[GA]AC
HindII        484  GTCAAC               GT[CT]^[GA]AC
HinfI         654  GACTC                G^A[ACGT]TC
Ksp632I       409  CTCTTC               CTCTTC(1/4)
KspI          242  CCGCGG               CCGC^GG
MboI          564  GATC                 ^GATC
MflI          563  GGATCC               [GA]^GATC[CT]
NaeI          298  GCCGGC               GCC^GGC
NdeII         564  GATC                 ^GATC
NgoMIV        298  GCCGGC               G^CCGGC
NspI          614  GCATGC               [GA]CATG^[CT]
PflFI         190  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          654  GACTC                GAGTC(4/5)
PstI          214  CTGCAG               CTGCA^G
PvuII         352  CAGCTG               CAG^CTG
SacI          406  GAGCTC               GAGCT^C
SacII         242  CCGCGG               CCGC^GG
SapI          408  GCTCTTC              GCTCTTC(1/4)
Sau3AI        564  GATC                 ^GATC
SfcI          214  CTGCAG               C^T[GA][CT]AG
SphI          614  GCATGC               GCATG^C
Tth111I       190  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         563  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwI          564  GATCC                GGATC(4/5)
              563  GGATC               
BbeI          106  GGCGCC               GGCGC^C
              235  GGCGCC              
BmyI          160  GGGCAC               G[AGT]GC[ACT]^C
              406  GAGCTC              
BseRI         147  CTCCTC               GAGGAG(10/8)
              403  GAGGAG              
BsgI          355  CTGCAC               GTGCAG(16/14)
              553  CTGCAC              
Bsp1286I      160  GGGCAC               G[AGT]GC[ACT]^C
              406  GAGCTC              
BsrFI         298  GCCGGC               [GA]^CCGG[CT]
              545  ACCGGC              
Cfr10I        298  GCCGGC               [GA]^CCGG[CT]
              545  ACCGGC              
DsaI          242  CCGCGG               C^C[GA][CT]GG
              392  CCACGG              
HaeII         106  GGCGCC               [GA]GCGC^[CT]
              235  GGCGCC              
KasI          106  GGCGCC               G^GCGCC
              235  GGCGCC              
MslI          393  CACGGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              468  CATGTGTGTG          
NarI          106  GGCGCC               GG^CGCC
              235  GGCGCC              
NlaIII        468  CATG                 CATG^
              615  CATG                
PpuMI         188  GGGACCC              [GA]G^G[AT]CC[CT]
              202  AGGACCT             
SfoI          106  GGCGCC               GGC^GCC
              235  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        AvaII       BanI        BbvI        BcnI        
BsaHI       BsaJI       BsiYI       BslI        BsmFI       
BsoBI       BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       SmaI        TseI        XmaCI       
XmaI        
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