################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BlnI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1720 GACGTC GACGT^C AflIII 1017 ACATGT A^C[GA][CT]GT Alw44I 256 GTGCAC G^TGCAC AocI 1132 CCTGAGG CC^T[ACGT]AGG ApaLI 256 GTGCAC G^TGCAC AvaI 467 CCCGAG C^[CT]CG[GA]G AviII 1577 TGCGCA TGC^GCA BbsI 757 GAAGAC GAAGAC(2/6) BbvCI 1234 GCTGAGG CCTCAGC(-5/-2) BclI 962 TGATCA T^GATCA BcnI 1593 CCCGG CC^[GC]GG BglI 1735 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 974 AGATCT A^GATCT BlpI 1111 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1111 GCTCAGC GC^T[ACGT]AGC BpuAI 757 GAAGAC GAAGAC(2/6) BsaAI 253 TACGTG [CT]AC^GT[GA] BsaBI 1182 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 298 GAGGAG GAGGAG(10/8) BsgI 1486 CTGCAC GTGCAG(16/14) BsiEI 8 CGGCCG CG[GA][CT]^CG BsoBI 467 CCCGAG C^[CT]CG[GA]G BspLU11I 1017 ACATGT A^CATGT BspMI 1484 ACCTGC ACCTGC(4/8) BsrDI 62 GCAATG GCAATG(2/0) BssSI 349 CACGAG CACGAG(-5/-1) Bsu36I 1132 CCTGAGG CC^T[ACGT]AGG CelII 1111 GCTCAGC GC^T[ACGT]AGC DraIII 379 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1749 GACTGTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 8 CGGCCG C^GGCCG EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG Eco81I 1132 CCTGAGG CC^T[ACGT]AGG FbaI 962 TGATCA T^GATCA FspI 1577 TGCGCA TGC^GCA MamI 1182 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1328 TTAA T^TAA NciI 1593 CCCGG CC^[GC]GG NcoI 536 CCATGG C^CATGG PflMI 1710 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1630 CTGCAG CTGCA^G SacI 805 GAGCTC GAGCT^C SapI 1503 GCTCTTC GCTCTTC(1/4) ScaI 503 AGTACT AGT^ACT SexAI 845 ACCTGGT A^CC[AT]GGT SfcI 1630 CTGCAG C^T[GA][CT]AG Tru9I 1328 TTAA T^TAA Van91I 1710 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccIII 509 TCCGGA T^CCGGA 1468 TCCGGA AcyI 1240 GACGCC G[GA]^CG[CT]C 1720 GACGTC AlwI 714 GGATC GGATC(4/5) 1356 GGATC ApaI 1430 GGGCCC GGGCC^C 1642 GGGCCC AspI 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 844 GACCTGGTC BsaHI 1240 GACGCC G[GA]^CG[CT]C 1720 GACGTC BsaI 1472 GAGACC GGTCTC(1/5) 1051 GGTCTC BsaWI 509 TCCGGA [AT]^CCGG[AT] 1468 TCCGGA BseAI 509 TCCGGA T^CCGGA 1468 TCCGGA BspEI 509 TCCGGA T^CCGGA 1468 TCCGGA BstYI 714 GGATCT [GA]^GATC[CT] 974 AGATCT DsaI 536 CCATGG C^C[GA][CT]GG 1568 CCACGG HgaI 1240 GACGC GACGC(5/10) 773 GCGTC Hin1I 1240 GACGCC G[GA]^CG[CT]C 1720 GACGTC MflI 714 GGATCT [GA]^GATC[CT] 974 AGATCT MroI 509 TCCGGA T^CCGGA 1468 TCCGGA PflFI 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 844 GACCTGGTC TaqI 498 TCGA T^CGA 1085 TCGA Tth111I 595 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 844 GACCTGGTC TthHB8I 498 TCGA T^CGA 1085 TCGA XhoII 714 GGATCT [GA]^GATC[CT] 974 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaII BalI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI XcmI