Restriction map for
pF1KB3219
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII AccIII AclI AcsI AflII
AhdI ApoI AseI AsnI AspEI
AspI AvrII BamHI BbvCI BciVI
BfaI BfrI BglI BlnI BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI MaeI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1885 GGTACC G^GTACC
AccI 1564 GTCTAC GT^[AC][GT]AC
AflIII 452 ACACGT A^C[GA][CT]GT
AgeI 185 ACCGGT A^CCGGT
AocI 799 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1220 AGCGCT AGC^GCT
AscI 247 GGCGCGCC GG^CGCGCC
Asp700I 1599 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1885 GGTACC G^GTACC
BbeI 517 GGCGCC GGCGC^C
BcgI 1367 GCACCCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1088 TGATCA T^GATCA
BsaI 235 GAGACC GGTCTC(1/5)
BsiEI 72 CGGCCG CG[GA][CT]^CG
BsmI 1791 GAATGC GAATGC(1/-1)
Bsp1407I 20 TGTACA T^GTACA
BsrGI 20 TGTACA T^GTACA
BssSI 468 CTCGTG CACGAG(-5/-1)
BstAPI 597 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 799 CCTCAGG CC^T[ACGT]AGG
EagI 72 CGGCCG C^GGCCG
EarI 1321 CTCTTC CTCTTC(1/4)
EclXI 72 CGGCCG C^GGCCG
Eco47III 1220 AGCGCT AGC^GCT
Eco52I 72 CGGCCG C^GGCCG
Eco81I 799 CCTCAGG CC^T[ACGT]AGG
EcoNI 699 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1088 TGATCA T^GATCA
HincII 1060 GTCAAC GT[CT]^[GA]AC
HindII 1060 GTCAAC GT[CT]^[GA]AC
KasI 517 GGCGCC G^GCGCC
KpnI 1885 GGTACC GGTAC^C
Ksp632I 1321 CTCTTC CTCTTC(1/4)
KspI 312 CCGCGG CCGC^GG
MseI 956 TTAA T^TAA
NarI 517 GGCGCC GG^CGCC
NcoI 974 CCATGG C^CATGG
PinAI 185 ACCGGT A^CCGGT
SacII 312 CCGCGG CCGC^GG
SfoI 517 GGCGCC GGC^GCC
SmaI 1931 CCCGGG CCC^GGG
SmlI 1576 CTCAAG C^T[CT][GA]AG
SnaBI 918 TACGTA TAC^GTA
SphI 1112 GCATGC GCATG^C
SspBI 20 TGTACA T^GTACA
Tru9I 956 TTAA T^TAA
XmaCI 1931 CCCGGG C^CCGGG
XmaI 1931 CCCGGG C^CCGGG
XmnI 1599 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 517 GGCGCC G[GA]^CG[CT]C
1822 GACGCC
Alw44I 220 GTGCAC G^TGCAC
1527 GTGCAC
ApaLI 220 GTGCAC G^TGCAC
1527 GTGCAC
AvaI 1206 CTCGGG C^[CT]CG[GA]G
1931 CCCGGG
AviII 792 TGCGCA TGC^GCA
1109 TGCGCA
BbrPI 453 CACGTG CAC^GTG
1684 CACGTG
BbsI 766 GAAGAC GAAGAC(2/6)
1842 GAAGAC
BglII 939 AGATCT A^GATCT
1980 AGATCT
BlpI 66 GCTCAGC GC^T[ACGT]AGC
745 GCTCAGC
Bpu1102I 66 GCTCAGC GC^T[ACGT]AGC
745 GCTCAGC
BpuAI 766 GAAGAC GAAGAC(2/6)
1842 GAAGAC
BsaHI 517 GGCGCC G[GA]^CG[CT]C
1822 GACGCC
BsaWI 185 ACCGGT [AT]^CCGG[AT]
1014 ACCGGA
BseRI 822 CTCCTC GAGGAG(10/8)
1072 GAGGAG
BsmAI 235 GAGAC GTCTC(1/5)
1457 GAGAC
BsmFI 869 GTCCC GGGAC(10/14)
1148 GTCCC
BsoBI 1206 CTCGGG C^[CT]CG[GA]G
1931 CCCGGG
BspHI 461 TCATGA T^CATGA
1787 TCATGA
BssHII 248 GCGCGC G^CGCGC
1583 GCGCGC
CelII 66 GCTCAGC GC^T[ACGT]AGC
745 GCTCAGC
FspI 792 TGCGCA TGC^GCA
1109 TGCGCA
HgaI 1822 GACGC GACGC(5/10)
984 GCGTC
Hin1I 517 GGCGCC G[GA]^CG[CT]C
1822 GACGCC
NaeI 115 GCCGGC GCC^GGC
277 GCCGGC
NgoMIV 115 GCCGGC G^CCGGC
277 GCCGGC
NspI 1112 GCATGC [GA]CATG^[CT]
1253 ACATGC
PmaCI 453 CACGTG CAC^GTG
1684 CACGTG
PmlI 453 CACGTG CAC^GTG
1684 CACGTG
RcaI 461 TCATGA T^CATGA
1787 TCATGA
SexAI 176 ACCTGGT A^CC[AT]GGT
401 ACCTGGT
TfiI 850 GAATC G^A[AT]TC
1670 GATTC
Tsp509I 905 AATT ^AATT
1213 AATT
XcmI 1067 CCACCGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1352 CCAAAGGCAGCCTGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII