Restriction map for
pF1KB3230
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbeI
BbrPI BcgI BfrI BglI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI NarI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1774 GACGTC GACGT^C
AccIII 495 TCCGGA T^CCGGA
AflIII 633 ACATGT A^C[GA][CT]GT
Alw44I 1234 GTGCAC G^TGCAC
AlwNI 81 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 277 GGGCCC GGGCC^C
ApaLI 1234 GTGCAC G^TGCAC
AvaI 986 CCCGAG C^[CT]CG[GA]G
BanI 1252 GGCACC G^G[CT][GA]CC
BbvCI 192 CCTCAGC CCTCAGC(-5/-2)
BciVI 415 GTATCC GTATCC(6/5)
BclI 855 TGATCA T^GATCA
BcnI 510 CCGGG CC^[GC]GG
BlpI 772 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 772 GCTCAGC GC^T[ACGT]AGC
BseAI 495 TCCGGA T^CCGGA
BseRI 676 GAGGAG GAGGAG(10/8)
BsmFI 1383 GTCCC GGGAC(10/14)
BsoBI 986 CCCGAG C^[CT]CG[GA]G
BspEI 495 TCCGGA T^CCGGA
BspLU11I 633 ACATGT A^CATGT
CelII 772 GCTCAGC GC^T[ACGT]AGC
DraI 1342 TTTAAA TTT^AAA
DsaI 905 CCATGG C^C[GA][CT]GG
EaeI 1500 TGGCCG [CT]^GGCC[GA]
EcoT14I 905 CCATGG C^C[AT][AT]GG
EcoT22I 647 ATGCAT ATGCA^T
FbaI 855 TGATCA T^GATCA
HaeII 1611 GGCGCT [GA]GCGC^[CT]
HincII 629 GTCAAC GT[CT]^[GA]AC
HindII 629 GTCAAC GT[CT]^[GA]AC
MroI 495 TCCGGA T^CCGGA
NciI 510 CCGGG CC^[GC]GG
NcoI 905 CCATGG C^CATGG
NdeI 1070 CATATG CA^TATG
NsiI 647 ATGCAT ATGCA^T
PshAI 396 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1243 CTGCAG CTGCA^G
PvuII 1879 CAGCTG CAG^CTG
SapI 1051 GAAGAGC GCTCTTC(1/4)
SmlI 217 CTCAAG C^T[CT][GA]AG
StyI 905 CCATGG C^C[AT][AT]GG
XbaI 207 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 863 CGCG CG^CG
1693 CGCG
AcyI 319 GACGCC G[GA]^CG[CT]C
1774 GACGTC
AviII 73 TGCGCA TGC^GCA
1785 TGCGCA
BamHI 372 GGATCC G^GATCC
1350 GGATCC
BbsI 393 GAAGAC GAAGAC(2/6)
1323 GAAGAC
BpuAI 393 GAAGAC GAAGAC(2/6)
1323 GAAGAC
BsaHI 319 GACGCC G[GA]^CG[CT]C
1774 GACGTC
BsaWI 495 TCCGGA [AT]^CCGG[AT]
1150 ACCGGA
BsgI 1059 CTGCAC GTGCAG(16/14)
1869 CTGCAC
BspMI 25 ACCTGC ACCTGC(4/8)
172 ACCTGC
BsrFI 176 GCCGGC [GA]^CCGG[CT]
1502 GCCGGC
BstAPI 791 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1236 GCACGGTCTGC
BstUI 863 CGCG CG^CG
1693 CGCG
BstYI 372 GGATCC [GA]^GATC[CT]
1350 GGATCC
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
1502 GCCGGC
DraII 53 AGGACCC [GA]G^G[ACGT]CC[CT]
347 AGGTCCT
DraIII 119 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1809 CACCGTGTG
EcoO109I 53 AGGACCC [GA]G^G[ACGT]CC[CT]
347 AGGTCCT
FspI 73 TGCGCA TGC^GCA
1785 TGCGCA
HgaI 319 GACGC GACGC(5/10)
385 GACGC
Hin1I 319 GACGCC G[GA]^CG[CT]C
1774 GACGTC
MflI 372 GGATCC [GA]^GATC[CT]
1350 GGATCC
MseI 578 TTAA T^TAA
1343 TTAA
MspA1I 1620 CCGCTG C[AC]G^C[GT]G
1879 CAGCTG
MvnI 863 CGCG CG^CG
1693 CGCG
NaeI 176 GCCGGC GCC^GGC
1502 GCCGGC
NgoMIV 176 GCCGGC G^CCGGC
1502 GCCGGC
NspI 180 GCATGT [GA]CATG^[CT]
633 ACATGT
PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT]
347 AGGTCCT
Tru9I 578 TTAA T^TAA
1343 TTAA
XcmI 131 CCAACGAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1458 CCAGGGGAGCTATGG
XhoII 372 GGATCC [GA]^GATC[CT]
1350 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I