Restriction map for
pF1KB3304
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRV FauI FseI
FspI HapII HgaI Hin1I HindIII
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 310 GTCTAC GT^[AC][GT]AC
AcsI 742 GAATTC [GA]^AATT[CT]
AflIII 1068 ACGTGT A^C[GA][CT]GT
Alw44I 114 GTGCAC G^TGCAC
AocI 147 CCTCAGG CC^T[ACGT]AGG
ApaLI 114 GTGCAC G^TGCAC
ApoI 742 GAATTC [GA]^AATT[CT]
Asp700I 1066 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1050 GGACC G^G[AT]CC
BanII 55 GAGCTC G[GA]GC[CT]^C
BbvCI 304 GCTGAGG CCTCAGC(-5/-2)
BclI 531 TGATCA T^GATCA
BfaI 1058 CTAG C^TAG
BsiEI 678 CGACCG CG[GA][CT]^CG
BsiYI 575 CCAGTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 575 CCAGTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 541 GAGACG CGTCTC(1/5)
BsmI 142 GCATTC GAATGC(1/-1)
BssKI 862 CCAGG ^CC[ACGT]GG
BstNI 862 CCAGG CC^[AT]GG
BstXI 260 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 147 CCTCAGG CC^T[ACGT]AGG
DpnII 532 GATC ^GATC
DraII 954 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 137 CCATGG C^C[GA][CT]GG
EaeI 374 TGGCCG [CT]^GGCC[GA]
EarI 928 GAAGAG CTCTTC(1/4)
Eco81I 147 CCTCAGG CC^T[ACGT]AGG
EcoO109I 954 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 742 GAATTC G^AATTC
EcoRII 862 CCAGG ^CC[AT]GG
EcoT14I 137 CCATGG C^C[AT][AT]GG
EcoT22I 806 ATGCAT ATGCA^T
FbaI 531 TGATCA T^GATCA
HincII 556 GTTAAC GT[CT]^[GA]AC
HindII 556 GTTAAC GT[CT]^[GA]AC
HpaI 556 GTTAAC GTT^AAC
Ksp632I 928 GAAGAG CTCTTC(1/4)
MaeI 1058 CTAG C^TAG
MaeIII 1131 GTCAC ^GT[ACGT]AC
MboI 532 GATC ^GATC
MslI 418 CATCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 896 CAGCTG C[AC]G^C[GT]G
MvaI 862 CCAGG CC^[AT]GG
NcoI 137 CCATGG C^CATGG
NdeII 532 GATC ^GATC
NsiI 806 ATGCAT ATGCA^T
PshAI 543 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 202 CTGCAG CTGCA^G
PvuII 896 CAGCTG CAG^CTG
SacI 55 GAGCTC GAGCT^C
Sau3AI 532 GATC ^GATC
ScrFI 862 CCAGG CC^[ACGT]GG
SmlI 228 CTCAAG C^T[CT][GA]AG
StyI 137 CCATGG C^C[AT][AT]GG
Tsp45I 1131 GTCAC ^GT[GC]AC
XmnI 1066 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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Aor51HI 84 AGCGCT AGC^GCT
301 AGCGCT
AspHI 55 GAGCTC G[AT]GC[AT]^C
114 GTGCAC
BmyI 55 GAGCTC G[AGT]GC[ACT]^C
114 GTGCAC
BsaI 132 GAGACC GGTCTC(1/5)
465 GGTCTC
BsaJI 137 CCATGG C^C[ACGT][ACGT]GG
862 CCAGGG
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
114 GTGCAC
Bsp1286I 55 GAGCTC G[AGT]GC[ACT]^C
114 GTGCAC
BssSI 1001 CTCGTG CACGAG(-5/-1)
1022 CTCGTG
Cac8I 34 GCACGC GC[ACGT]^[ACGT]GC
211 GCAGGC
CfoI 85 GCGC GCG^C
302 GCGC
Eco47III 84 AGCGCT AGC^GCT
301 AGCGCT
HaeII 84 AGCGCT [GA]GCGC^[CT]
301 AGCGCT
HhaI 85 GCGC GCG^C
302 GCGC
HinP1I 85 GCGC G^CGC
302 GCGC
HphI 502 GGTGA GGTGA(8/7)
973 GGTGA
MwoI 295 GCTATCAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
376 GCCGCTTTAGC
SfcI 202 CTGCAG C^T[GA][CT]AG
690 CTATAG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BseRI BsgI BsmAI BsrI BstF5I
Cfr13I DdeI Fnu4HI FokI HaeIII
HinfI ItaI MaeII MboII MnlI
MseI NlaIII NlaIV PleI RsaI
Sau96I SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I