Restriction map for
pF1KB3312
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeII MluI MluNI MroI MscI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 366 ACATGT A^C[GA][CT]GT
AlwNI 485 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1078 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1980 AGCGCT AGC^GCT
BamHI 883 GGATCC G^GATCC
BbsI 994 GTCTTC GAAGAC(2/6)
BciVI 2067 GGATAC GTATCC(6/5)
BglII 1610 AGATCT A^GATCT
BpmI 875 CTGGAG CTGGAG(16/14)
BpuAI 994 GTCTTC GAAGAC(2/6)
BsaBI 2293 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 446 GGTCTC GGTCTC(1/5)
BsaWI 937 ACCGGA [AT]^CCGG[AT]
BseRI 2362 GAGGAG GAGGAG(10/8)
BspLU11I 366 ACATGT A^CATGT
BspMI 218 ACCTGC ACCTGC(4/8)
BsrBI 147 CCGCTC CCGCTC(-3/-3)
BsrDI 207 GCAATG GCAATG(2/0)
BstBI 1871 TTCGAA TT^CGAA
BstXI 1699 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1078 CCTCAGG CC^T[ACGT]AGG
CfoI 1981 GCGC GCG^C
DraII 1519 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1001 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 972 CGGCCA [CT]^GGCC[GA]
EarI 1299 CTCTTC CTCTTC(1/4)
Eco47III 1980 AGCGCT AGC^GCT
Eco81I 1078 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1519 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1987 GATATC GAT^ATC
EcoT14I 1166 CCAAGG C^C[AT][AT]GG
HaeII 1980 AGCGCT [GA]GCGC^[CT]
HapII 938 CCGG C^CGG
HhaI 1981 GCGC GCG^C
HinP1I 1981 GCGC G^CGC
HindIII 250 AAGCTT A^AGCTT
HpaII 938 CCGG C^CGG
Ksp632I 1299 CTCTTC CTCTTC(1/4)
MamI 2293 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 938 CCGG C^CGG
NspV 1871 TTCGAA TT^CGAA
ScaI 668 AGTACT AGT^ACT
SfuI 1871 TTCGAA TT^CGAA
SpeI 1147 ACTAGT A^CTAGT
StyI 1166 CCAAGG C^C[AT][AT]GG
SwaI 501 ATTTAAAT ATTT^AAAT
Tsp45I 1151 GTGAC ^GT[GC]AC
XcmI 1482 CCACTACAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 1127 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1722 GAACTGTTTC
AvaII 811 GGACC G^G[AT]CC
1057 GGTCC
BanII 941 GAGCCC G[GA]GC[CT]^C
1089 GAGCCC
BfaI 1148 CTAG C^TAG
1895 CTAG
BmyI 941 GAGCCC G[AGT]GC[ACT]^C
1089 GAGCCC
BsmAI 595 GAGAC GTCTC(1/5)
447 GTCTC
BsmFI 363 GGGAC GGGAC(10/14)
733 GTCCC
Bsp1286I 941 GAGCCC G[AGT]GC[ACT]^C
1089 GAGCCC
BstF5I 932 CATCC GGATG(2/0)
2433 GGATG
DraI 429 TTTAAA TTT^AAA
502 TTTAAA
EcoT22I 1907 ATGCAT ATGCA^T
1966 ATGCAT
FokI 932 CATCC GGATG(9/13)
2433 GGATG
MaeI 1148 CTAG C^TAG
1895 CTAG
MfeI 626 CAATTG C^AATTG
2333 CAATTG
MslI 203 CACCGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1900 CATTATAATG
MspA1I 710 CAGCTG C[AC]G^C[GT]G
2453 CAGCTG
MunI 626 CAATTG C^AATTG
2333 CAATTG
MwoI 160 GCATATAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1705 GCCTGGTGTGC
NsiI 1907 ATGCAT ATGCA^T
1966 ATGCAT
PstI 10 CTGCAG CTGCA^G
1715 CTGCAG
PvuII 710 CAGCTG CAG^CTG
2453 CAGCTG
SfcI 10 CTGCAG C^T[GA][CT]AG
1715 CTGCAG
SspI 1456 AATATT AAT^ATT
1560 AATATT
StuI 1349 AGGCCT AGG^CCT
1703 AGGCCT
XmnI 1127 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1722 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BsiYI BslI
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XhoII