Restriction map for
pF1KB3338
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AclI AcsI AflII AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
BbeI BfrI BsaI BsiEI BsiWI
BsmBI BspHI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 3082 GACGTC GACGT^C
AccIII 614 TCCGGA T^CCGGA
AgeI 1907 ACCGGT A^CCGGT
AocI 247 CCTGAGG CC^T[ACGT]AGG
AviII 199 TGCGCA TGC^GCA
AvrII 1489 CCTAGG C^CTAGG
BamHI 645 GGATCC G^GATCC
BbrPI 931 CACGTG CAC^GTG
BcgI 2464 GCAGGGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1140 GTATCC GTATCC(6/5)
BclI 2771 TGATCA T^GATCA
BlnI 1489 CCTAGG C^CTAGG
BsaAI 931 CACGTG [CT]AC^GT[GA]
BsaBI 444 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 614 TCCGGA T^CCGGA
BsmI 3003 GCATTC GAATGC(1/-1)
Bsp1407I 3365 TGTACA T^GTACA
BspDI 2849 ATCGAT AT^CGAT
BspEI 614 TCCGGA T^CCGGA
BsrGI 3365 TGTACA T^GTACA
BstAPI 2570 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 247 CCTGAGG CC^T[ACGT]AGG
ClaI 2849 ATCGAT AT^CGAT
Eco81I 247 CCTGAGG CC^T[ACGT]AGG
FbaI 2771 TGATCA T^GATCA
FspI 199 TGCGCA TGC^GCA
HincII 2584 GTCAAC GT[CT]^[GA]AC
HindII 2584 GTCAAC GT[CT]^[GA]AC
HindIII 3370 AAGCTT A^AGCTT
MamI 444 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 614 TCCGGA T^CCGGA
NheI 1227 GCTAGC G^CTAGC
PflMI 1316 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1907 ACCGGT A^CCGGT
PmaCI 931 CACGTG CAC^GTG
PmlI 931 CACGTG CAC^GTG
SexAI 1253 ACCTGGT A^CC[AT]GGT
SspBI 3365 TGTACA T^GTACA
Van91I 1316 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2726 CCACCTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 386 CGCG CG^CG
3154 CGCG
AspI 448 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3283 GACGAGGTC
BanI 1129 GGTGCC G^G[CT][GA]CC
2617 GGCACC
BbvCI 2343 CCTCAGC CCTCAGC(-5/-2)
2868 CCTCAGC
BglI 1615 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1804 GCCGTGGGGGC
BglII 992 AGATCT A^GATCT
2880 AGATCT
BlpI 911 GCTCAGC GC^T[ACGT]AGC
2842 GCTGAGC
Bpu1102I 911 GCTCAGC GC^T[ACGT]AGC
2842 GCTGAGC
BsaWI 614 TCCGGA [AT]^CCGG[AT]
1907 ACCGGT
BseRI 67 GAGGAG GAGGAG(10/8)
3106 GAGGAG
BspLU11I 260 ACATGT A^CATGT
1172 ACATGT
BspMI 2234 ACCTGC ACCTGC(4/8)
2419 GCAGGT
BstUI 386 CGCG CG^CG
3154 CGCG
BstXI 1928 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3305 CCATCATCATGG
CelII 911 GCTCAGC GC^T[ACGT]AGC
2842 GCTGAGC
DrdI 1948 GACGCCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2329 GACCTGGGTGTC
FauI 231 CCCGC CCCGC(4/6)
1271 CCCGC
MseI 497 TTAA T^TAA
2897 TTAA
MvnI 386 CGCG CG^CG
3154 CGCG
PflFI 448 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3283 GACGAGGTC
PshAI 2035 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2480 GACGGGTGTC
ScaI 572 AGTACT AGT^ACT
2798 AGTACT
SfiI 1614 GGCCATTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1803 GGCCGTGGGGGCC
SmaI 1626 CCCGGG CCC^GGG
1814 CCCGGG
StuI 666 AGGCCT AGG^CCT
2355 AGGCCT
Tru9I 497 TTAA T^TAA
2897 TTAA
Tsp509I 611 AATT ^AATT
3198 AATT
Tth111I 448 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3283 GACGAGGTC
XmaCI 1626 CCCGGG C^CCGGG
1814 CCCGGG
XmaI 1626 CCCGGG C^CCGGG
1814 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII