Restriction map for
pF1KB3349
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# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AclI AscI AseI
AsnI Asp718I BbvCI BciVI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII EcoRV EcoT22I
FseI HpaI KpnI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SbfI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 3872 TCCGGA T^CCGGA
AflII 1731 CTTAAG C^TTAAG
AgeI 3575 ACCGGT A^CCGGT
AocI 3027 CCTGAGG CC^T[ACGT]AGG
Asp700I 3207 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1341 CCTAGG C^CTAGG
BbeI 3807 GGCGCC GGCGC^C
BbrPI 3854 CACGTG CAC^GTG
BcgI 171 GCACCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1731 CTTAAG C^TTAAG
BglII 491 AGATCT A^GATCT
BlnI 1341 CCTAGG C^CTAGG
BsaBI 675 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3872 TCCGGA T^CCGGA
BsiEI 3895 CGGCCG CG[GA][CT]^CG
BsmBI 246 CGTCTC CGTCTC(1/5)
BspEI 3872 TCCGGA T^CCGGA
BsrDI 4530 CATTGC GCAATG(2/0)
BssHII 1419 GCGCGC G^CGCGC
BssSI 4297 CTCGTG CACGAG(-5/-1)
Bsu36I 3027 CCTGAGG CC^T[ACGT]AGG
EagI 3895 CGGCCG C^GGCCG
EclXI 3895 CGGCCG C^GGCCG
Eco52I 3895 CGGCCG C^GGCCG
Eco81I 3027 CCTGAGG CC^T[ACGT]AGG
EcoRI 3056 GAATTC G^AATTC
HindIII 952 AAGCTT A^AGCTT
KasI 3807 GGCGCC G^GCGCC
KspI 2336 CCGCGG CCGC^GG
MamI 675 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3872 TCCGGA T^CCGGA
NaeI 4233 GCCGGC GCC^GGC
NarI 3807 GGCGCC GG^CGCC
NgoMIV 4233 GCCGGC G^CCGGC
PaeR7I 2285 CTCGAG C^TCGAG
PinAI 3575 ACCGGT A^CCGGT
PmaCI 3854 CACGTG CAC^GTG
PmlI 3854 CACGTG CAC^GTG
SacII 2336 CCGCGG CCGC^GG
SalI 604 GTCGAC G^TCGAC
SapI 3327 GCTCTTC GCTCTTC(1/4)
ScaI 3785 AGTACT AGT^ACT
SexAI 3908 ACCTGGT A^CC[AT]GGT
SfiI 903 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3807 GGCGCC GGC^GCC
SmaI 3666 CCCGGG CCC^GGG
XhoI 2285 CTCGAG C^TCGAG
XmaCI 3666 CCCGGG C^CCGGG
XmaI 3666 CCCGGG C^CCGGG
XmnI 3207 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 604 GTCGAC GT^[AC][GT]AC
3008 GTAGAC
AcsI 3056 GAATTC [GA]^AATT[CT]
4507 GAATTT
AflIII 1661 ACGTGT A^C[GA][CT]GT
4214 ACACGT
AhdI 715 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2326 GACCCACTGTC
ApoI 3056 GAATTC [GA]^AATT[CT]
4507 GAATTT
AspEI 715 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2326 GACCCACTGTC
AspI 2371 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2747 GACAGGGTC
AviII 1574 TGCGCA TGC^GCA
4139 TGCGCA
BamHI 1802 GGATCC G^GATCC
2096 GGATCC
BclI 1004 TGATCA T^GATCA
1643 TGATCA
BglI 904 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1693 GCCGAGAAGGC
BlpI 993 GCTCAGC GC^T[ACGT]AGC
3582 GCTGAGC
Bpu1102I 993 GCTCAGC GC^T[ACGT]AGC
3582 GCTGAGC
BsaI 625 GAGACC GGTCTC(1/5)
3920 GAGACC
BsaWI 3575 ACCGGT [AT]^CCGG[AT]
3872 TCCGGA
BstAPI 759 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3631 GCACTATTTGC
BstEII 339 GGTCACC G^GT[ACGT]ACC
1635 GGTGACC
BstPI 339 GGTCACC G^GT[ACGT]ACC
1635 GGTGACC
CelII 993 GCTCAGC GC^T[ACGT]AGC
3582 GCTGAGC
DrdI 1942 GACATCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3223 GACATCTATGTC
Eam1105I 715 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2326 GACCCACTGTC
EcoO65I 339 GGTCACC G^GT[ACGT]ACC
1635 GGTGACC
FbaI 1004 TGATCA T^GATCA
1643 TGATCA
FspI 1574 TGCGCA TGC^GCA
4139 TGCGCA
HincII 604 GTCGAC GT[CT]^[GA]AC
1228 GTCAAC
HindII 604 GTCGAC GT[CT]^[GA]AC
1228 GTCAAC
NheI 2187 GCTAGC G^CTAGC
2469 GCTAGC
NspI 1748 ACATGC [GA]CATG^[CT]
3933 GCATGT
PflFI 2371 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2747 GACAGGGTC
PshAI 2480 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3922 GACCTGAGTC
Tth111I 2371 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2747 GACAGGGTC
XcmI 209 CCAAGCTCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2849 CCATTGGCACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII