Restriction map for
pF1KB3408
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI AspEI AvrII
BbrPI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrGI BstBI
CelII ClaI DraI DrdI Eam1105I
EcoNI EcoRI EcoT22I FseI HincII
HindII HindIII HpaI KspI MaeI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacII
SalI ScaI SfiI SfuI SgrAI
SpeI SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1156 GGTACC G^GTACC
AccIII 704 TCCGGA T^CCGGA
AflIII 1358 ACGTGT A^C[GA][CT]GT
AocI 1813 CCTGAGG CC^T[ACGT]AGG
Asp718I 1156 GGTACC G^GTACC
AspI 430 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 185 TGCGCA TGC^GCA
BamHI 416 GGATCC G^GATCC
BclI 1868 TGATCA T^GATCA
BsaBI 2145 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 704 TCCGGA T^CCGGA
BspEI 704 TCCGGA T^CCGGA
BspHI 1865 TCATGA T^CATGA
BsrDI 2729 GCAATG GCAATG(2/0)
BssHII 92 GCGCGC G^CGCGC
Bst1107I 1164 GTATAC GTA^TAC
BstAPI 1428 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1164 GTATAC GTA^TAC
Bsu36I 1813 CCTGAGG CC^T[ACGT]AGG
CpoI 395 CGGACCG CG^G[AT]CCG
DraIII 2556 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2497 CTCTTC CTCTTC(1/4)
Eco81I 1813 CCTGAGG CC^T[ACGT]AGG
EcoRV 2916 GATATC GAT^ATC
FbaI 1868 TGATCA T^GATCA
FspI 185 TGCGCA TGC^GCA
KpnI 1156 GGTACC GGTAC^C
Ksp632I 2497 CTCTTC CTCTTC(1/4)
MamI 2145 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 704 TCCGGA T^CCGGA
MseI 2081 TTAA T^TAA
NaeI 2650 GCCGGC GCC^GGC
NgoMIV 2650 GCCGGC G^CCGGC
PflFI 430 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1865 TCATGA T^CATGA
RsrII 395 CGGACCG CG^G[AT]CCG
SacI 118 GAGCTC GAGCT^C
SapI 2496 GCTCTTC GCTCTTC(1/4)
SbfI 1374 CCTGCAGG CCTGCA^GG
SmlI 2950 CTCAAG C^T[CT][GA]AG
SnaBI 2527 TACGTA TAC^GTA
SphI 1793 GCATGC GCATG^C
Sse8387I 1374 CCTGCAGG CCTGCA^GG
TfiI 547 GAATC G^A[AT]TC
Tru9I 2081 TTAA T^TAA
Tth111I 430 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 877 GTCTAC GT^[AC][GT]AC
1164 GTATAC
Aor51HI 101 AGCGCT AGC^GCT
1568 AGCGCT
BbeI 1405 GGCGCC GGCGC^C
2891 GGCGCC
BbsI 1936 GAAGAC GAAGAC(2/6)
2632 GAAGAC
BbvCI 1980 CCTCAGC CCTCAGC(-5/-2)
2607 GCTGAGG
BcgI 1110 CGAGGCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2085 CGAGATCACTGC
BpuAI 1936 GAAGAC GAAGAC(2/6)
2632 GAAGAC
BsaAI 1161 CACGTA [CT]AC^GT[GA]
2527 TACGTA
BsaWI 704 TCCGGA [AT]^CCGG[AT]
1469 ACCGGA
BsmBI 2745 CGTCTC CGTCTC(1/5)
1762 GAGACG
BstEII 84 GGTGACC G^GT[ACGT]ACC
2874 GGTGACC
BstPI 84 GGTGACC G^GT[ACGT]ACC
2874 GGTGACC
EagI 23 CGGCCG C^GGCCG
2171 CGGCCG
EclXI 23 CGGCCG C^GGCCG
2171 CGGCCG
Eco47III 101 AGCGCT AGC^GCT
1568 AGCGCT
Eco52I 23 CGGCCG C^GGCCG
2171 CGGCCG
EcoO65I 84 GGTGACC G^GT[ACGT]ACC
2874 GGTGACC
KasI 1405 GGCGCC G^GCGCC
2891 GGCGCC
NarI 1405 GGCGCC GG^CGCC
2891 GGCGCC
PflMI 2555 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2895 CCAGTGGGTGG
PleI 1264 GACTC GAGTC(4/5)
2674 GAGTC
PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT]
2583 GGGACCT
SexAI 2387 ACCTGGT A^CC[AT]GGT
2557 ACCTGGT
SfoI 1405 GGCGCC GGC^GCC
2891 GGCGCC
Tsp509I 2276 AATT ^AATT
2337 AATT
Van91I 2555 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2895 CCAGTGGGTGG
XcmI 299 CCAACGCCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
512 CCACGCTGCGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI XmnI