Restriction map for
pF1KB3411
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvrII
BalI BamHI BbeI BbrPI BfrI
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FseI HindIII
HpaI KasI MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 2036 GGTACC G^GTACC
AccIII 866 TCCGGA T^CCGGA
AocI 2686 CCTCAGG CC^T[ACGT]AGG
Asp718I 2036 GGTACC G^GTACC
BbvCI 2538 GCTGAGG CCTCAGC(-5/-2)
BcgI 1320 GCAGCGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1803 GTATCC GTATCC(6/5)
BclI 1604 TGATCA T^GATCA
BglII 242 AGATCT A^GATCT
BsaAI 2415 CACGTA [CT]AC^GT[GA]
BsaWI 866 TCCGGA [AT]^CCGG[AT]
BseAI 866 TCCGGA T^CCGGA
BsiWI 2597 CGTACG C^GTACG
BspEI 866 TCCGGA T^CCGGA
BsrDI 336 GCAATG GCAATG(2/0)
BssSI 305 CTCGTG CACGAG(-5/-1)
BstAPI 332 GCAAGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2686 CCTCAGG CC^T[ACGT]AGG
CpoI 1820 CGGTCCG CG^G[AT]CCG
DrdI 1387 GACTACGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1220 TGGCCG [CT]^GGCC[GA]
EarI 997 GAAGAG CTCTTC(1/4)
Eco81I 2686 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1690 ATGCAT ATGCA^T
FbaI 1604 TGATCA T^GATCA
HincII 1558 GTCAAC GT[CT]^[GA]AC
HindII 1558 GTCAAC GT[CT]^[GA]AC
KpnI 2036 GGTACC GGTAC^C
Ksp632I 997 GAAGAG CTCTTC(1/4)
MroI 866 TCCGGA T^CCGGA
MseI 398 TTAA T^TAA
NdeI 1780 CATATG CA^TATG
NsiI 1690 ATGCAT ATGCA^T
NspI 417 GCATGT [GA]CATG^[CT]
PaeR7I 1328 CTCGAG C^TCGAG
PshAI 1500 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1820 CGGTCCG CG^G[AT]CCG
SmaI 1194 CCCGGG CCC^GGG
SmlI 1328 CTCGAG C^T[CT][GA]AG
TfiI 955 GATTC G^A[AT]TC
Tru9I 398 TTAA T^TAA
XcmI 266 CCAAGATCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1328 CTCGAG C^TCGAG
XmaCI 1194 CCCGGG C^CCGGG
XmaI 1194 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 2410 GTAGAC GT^[AC][GT]AC
2489 GTCTAC
AcyI 1393 GGCGTC G[GA]^CG[CT]C
1584 GACGCC
AflIII 947 ACGTGT A^C[GA][CT]GT
2414 ACACGT
Alw44I 1736 GTGCAC G^TGCAC
2692 GTGCAC
ApaLI 1736 GTGCAC G^TGCAC
2692 GTGCAC
AviII 2399 TGCGCA TGC^GCA
2453 TGCGCA
BglI 1178 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1346 GCCTGTTCGGC
BsaHI 1393 GGCGTC G[GA]^CG[CT]C
1584 GACGCC
BstEII 1947 GGTCACC G^GT[ACGT]ACC
1959 GGTCACC
BstPI 1947 GGTCACC G^GT[ACGT]ACC
1959 GGTCACC
EcoO65I 1947 GGTCACC G^GT[ACGT]ACC
1959 GGTCACC
FspI 2399 TGCGCA TGC^GCA
2453 TGCGCA
Hin1I 1393 GGCGTC G[GA]^CG[CT]C
1584 GACGCC
KspI 1259 CCGCGG CCGC^GG
1400 CCGCGG
PflMI 318 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2607 CCAGTCAGTGG
PstI 1318 CTGCAG CTGCA^G
2323 CTGCAG
SacI 1325 GAGCTC GAGCT^C
1754 GAGCTC
SacII 1259 CCGCGG CCGC^GG
1400 CCGCGG
SexAI 116 ACCTGGT A^CC[AT]GGT
281 ACCTGGT
StuI 812 AGGCCT AGG^CCT
1022 AGGCCT
Tsp509I 56 AATT ^AATT
395 AATT
Van91I 318 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2607 CCAGTCAGTGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I XhoII