Restriction map for
pF1KB3471
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BbrPI BbsI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
MfeI MroI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 754 GTCTAC GT^[AC][GT]AC
AflIII 377 ACGCGT A^C[GA][CT]GT
AhdI 841 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 870 CCTCAGG CC^T[ACGT]AGG
AspEI 841 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 257 TGCGCA TGC^GCA
BamHI 467 GGATCC G^GATCC
BcgI 332 GCAACGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1441 CTAG C^TAG
BglI 1260 GCCGTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 255 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 673 GAGAC GTCTC(1/5)
BsmBI 673 GAGACG CGTCTC(1/5)
BspMI 1559 ACCTGC ACCTGC(4/8)
BsrBI 781 CCGCTC CCGCTC(-3/-3)
BsrFI 1288 GCCGGC [GA]^CCGG[CT]
BsrI 846 CCAGT ACTGG(1/-1)
BstXI 1127 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 870 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1288 GCCGGC [GA]^CCGG[CT]
DraIII 1570 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 352 CCGCGG C^C[GA][CT]GG
Eam1105I 841 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 870 CCTCAGG CC^T[ACGT]AGG
EcoT14I 911 CCAAGG C^C[AT][AT]GG
FspI 257 TGCGCA TGC^GCA
HincII 1372 GTCAAC GT[CT]^[GA]AC
HindII 1372 GTCAAC GT[CT]^[GA]AC
KspI 352 CCGCGG CCGC^GG
MaeI 1441 CTAG C^TAG
MaeIII 849 GTCAC ^GT[ACGT]AC
MamI 255 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 377 ACGCGT A^CGCGT
NaeI 1288 GCCGGC GCC^GGC
NgoMIV 1288 GCCGGC G^CCGGC
NspI 1151 ACATGC [GA]CATG^[CT]
PpuMI 827 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1087 CTGCAG CTGCA^G
SacI 46 GAGCTC GAGCT^C
SacII 352 CCGCGG CCGC^GG
SexAI 1571 ACCTGGT A^CC[AT]GGT
SfcI 1087 CTGCAG C^T[GA][CT]AG
SmlI 940 CTCAAG C^T[CT][GA]AG
StyI 911 CCAAGG C^C[AT][AT]GG
Tsp45I 849 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 15 enzymes) #
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ApaI 265 GGGCCC GGGCC^C
701 GGGCCC
BclI 608 TGATCA T^GATCA
890 TGATCA
DdeI 314 CTGAG C^T[ACGT]AG
871 CTCAG
EcoNI 831 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1518 CCTCATCAAGG
FbaI 608 TGATCA T^GATCA
890 TGATCA
HinfI 283 GACTC G^A[ACGT]TC
1201 GAGTC
MaeII 1307 ACGT A^CGT
1466 ACGT
PflMI 54 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
573 CCACAAGCTGG
PleI 283 GACTC GAGTC(4/5)
1201 GAGTC
SapI 48 GCTCTTC GCTCTTC(1/4)
561 GCTCTTC
SmaI 249 CCCGGG CCC^GGG
463 CCCGGG
TspRI 312 CACTG CA[GC]TG[ACGT][ACGT]^
851 CACTG
Van91I 54 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
573 CCACAAGCTGG
XmaCI 249 CCCGGG C^CCGGG
463 CCCGGG
XmaI 249 CCCGGG C^CCGGG
463 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BglII BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI Ksp632I MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI TaqI TseI TthHB8I
XcmI XhoII