Restriction map for
pF1KB3473
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaII AviII BalI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HapII Hin1I
HpaII KasI KpnI Ksp632I KspI
MamI MluNI MroI MscI MspA1I
MspI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 204 GTATAC GT^[AC][GT]AC
AccII 1479 CGCG CG^CG
AclI 510 AACGTT AA^CGTT
AcsI 702 AAATTT [GA]^AATT[CT]
AflIII 1478 ACGCGT A^C[GA][CT]GT
Aor51HI 1000 AGCGCT AGC^GCT
ApoI 702 AAATTT [GA]^AATT[CT]
AvaI 1199 CTCGGG C^[CT]CG[GA]G
AvrII 1083 CCTAGG C^CTAGG
BamHI 1383 GGATCC G^GATCC
BbvCI 1098 CCTCAGC CCTCAGC(-5/-2)
BglII 807 AGATCT A^GATCT
BlnI 1083 CCTAGG C^CTAGG
BsaI 585 GAGACC GGTCTC(1/5)
BsmI 1040 GAATGC GAATGC(1/-1)
BsoBI 1199 CTCGGG C^[CT]CG[GA]G
BspHI 225 TCATGA T^CATGA
BspMI 607 GCAGGT ACCTGC(4/8)
Bst1107I 204 GTATAC GTA^TAC
BstEII 1485 GGTGACC G^GT[ACGT]ACC
BstPI 1485 GGTGACC G^GT[ACGT]ACC
BstUI 1479 CGCG CG^CG
BstZ17I 204 GTATAC GTA^TAC
Cfr13I 385 GGCCC G^G[ACGT]CC
DraII 384 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 767 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1387 CCACGG C^C[GA][CT]GG
EaeI 1419 TGGCCG [CT]^GGCC[GA]
Eco47III 1000 AGCGCT AGC^GCT
EcoO109I 384 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1485 GGTGACC G^GT[ACGT]ACC
EcoT14I 1083 CCTAGG C^C[AT][AT]GG
FauI 1293 CCCGC CCCGC(4/6)
HgaI 1477 GACGC GACGC(5/10)
HincII 513 GTTAAC GT[CT]^[GA]AC
HindII 513 GTTAAC GT[CT]^[GA]AC
HindIII 930 AAGCTT A^AGCTT
HpaI 513 GTTAAC GTT^AAC
MaeII 511 ACGT A^CGT
MfeI 1409 CAATTG C^AATTG
MluI 1478 ACGCGT A^CGCGT
MunI 1409 CAATTG C^AATTG
MvnI 1479 CGCG CG^CG
NheI 172 GCTAGC G^CTAGC
NspI 527 GCATGC [GA]CATG^[CT]
PshAI 1055 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 510 AACGTT AA^CGTT
RcaI 225 TCATGA T^CATGA
SacI 575 GAGCTC GAGCT^C
Sau96I 385 GGCCC G^G[ACGT]CC
ScaI 595 AGTACT AGT^ACT
SexAI 768 ACCAGGT A^CC[AT]GGT
SfcI 626 CTACAG C^T[GA][CT]AG
SphI 527 GCATGC GCATG^C
StuI 273 AGGCCT AGG^CCT
StyI 1083 CCTAGG C^C[AT][AT]GG
TaqI 813 TCGA T^CGA
TthHB8I 813 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 596 GTAC GT^AC
954 GTAC
AseI 81 ATTAAT AT^TAAT
1150 ATTAAT
AsnI 81 ATTAAT AT^TAAT
1150 ATTAAT
BanII 291 GAGCCC G[GA]GC[CT]^C
575 GAGCTC
BpmI 572 CTGGAG CTGGAG(16/14)
1115 CTGGAG
BseRI 578 CTCCTC GAGGAG(10/8)
1196 CTCCTC
BsmFI 1053 GGGAC GGGAC(10/14)
486 GTCCC
BsrDI 602 GCAATG GCAATG(2/0)
961 GCAATG
BstYI 807 AGATCT [GA]^GATC[CT]
1383 GGATCC
CfoI 1001 GCGC GCG^C
1323 GCGC
EcoNI 763 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
937 CCTCCGCAAGG
HaeII 1000 AGCGCT [GA]GCGC^[CT]
1322 GGCGCT
HhaI 1001 GCGC GCG^C
1323 GCGC
HinP1I 1001 GCGC G^CGC
1323 GCGC
HinfI 341 GATTC G^A[ACGT]TC
520 GATTC
HphI 1485 GGTGA GGTGA(8/7)
1192 TCACC
MflI 807 AGATCT [GA]^GATC[CT]
1383 GGATCC
MslI 177 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
556 CATAGAAATG
PshBI 81 ATTAAT AT^TAAT
1150 ATTAAT
RsaI 596 GTAC GT^AC
954 GTAC
TfiI 341 GATTC G^A[AT]TC
520 GATTC
XhoII 807 AGATCT [GA]^GATC[CT]
1383 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PflMI Sau3AI ScrFI SfaNI
SmlI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I