Restriction map for
pF1KB3505
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmFI BspDI BspEI
BspHI BspMI BsrFI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I Cfr10I
Cfr13I ClaI CpoI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeIII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MluI
MluNI MroI MscI MslI NaeI
NarI NcoI NgoMIV NlaIV NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfiI SfoI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 217 GTCTAC GT^[AC][GT]AC
AflIII 970 ACATGT A^C[GA][CT]GT
BanII 840 GAGCCC G[GA]GC[CT]^C
BbsI 922 GAAGAC GAAGAC(2/6)
BlpI 12 GCTGAGC GC^T[ACGT]AGC
BmyI 840 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
BpuAI 922 GAAGAC GAAGAC(2/6)
BsaI 1054 GGTCTC GGTCTC(1/5)
BseRI 190 GAGGAG GAGGAG(10/8)
BsiEI 57 CGACCG CG[GA][CT]^CG
BsiYI 66 CCTCCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 66 CCTCCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1055 GTCTC GTCTC(1/5)
BsmI 725 GAATGC GAATGC(1/-1)
Bsp1286I 840 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 405 TGTACA T^GTACA
BspLU11I 970 ACATGT A^CATGT
BsrDI 637 CATTGC GCAATG(2/0)
BsrGI 405 TGTACA T^GTACA
BsrI 234 CCAGT ACTGG(1/-1)
BssHII 91 GCGCGC G^CGCGC
BstAPI 361 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 643 TTCGAA TT^CGAA
BstNI 693 CCTGG CC^[AT]GG
BstXI 168 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 12 GCTGAGC GC^T[ACGT]AGC
DraI 586 TTTAAA TTT^AAA
DraIII 677 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 66 CCTCCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 693 CCTGG ^CC[AT]GG
EcoT22I 727 ATGCAT ATGCA^T
HaeII 16 AGCGCC [GA]GCGC^[CT]
HapII 297 CCGG C^CGG
HpaII 297 CCGG C^CGG
MfeI 143 CAATTG C^AATTG
MspA1I 896 CAGCTG C[AC]G^C[GT]G
MspI 297 CCGG C^CGG
MunI 143 CAATTG C^AATTG
MvaI 693 CCTGG CC^[AT]GG
NheI 327 GCTAGC G^CTAGC
NsiI 727 ATGCAT ATGCA^T
NspI 970 ACATGT [GA]CATG^[CT]
NspV 643 TTCGAA TT^CGAA
PvuII 896 CAGCTG CAG^CTG
SexAI 692 ACCTGGT A^CC[AT]GGT
SfuI 643 TTCGAA TT^CGAA
SmaI 296 CCCGGG CCC^GGG
SspBI 405 TGTACA T^GTACA
XmaCI 296 CCCGGG C^CCGGG
XmaI 296 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 92 CGCG CG^CG
94 CGCG
AvaI 296 CCCGGG C^[CT]CG[GA]G
428 CCCGAG
BcnI 296 CCCGG CC^[GC]GG
297 CCGGG
BglII 576 AGATCT A^GATCT
613 AGATCT
BpmI 332 CTGGAG CTGGAG(16/14)
371 CTGGAG
BsaJI 20 CCGAGG C^C[ACGT][ACGT]GG
296 CCCGGG
BsgI 6 CTGCAC GTGCAG(16/14)
994 GTGCAG
BsoBI 296 CCCGGG C^[CT]CG[GA]G
428 CCCGAG
BsrBI 40 GAGCGG CCGCTC(-3/-3)
619 GAGCGG
BstUI 92 CGCG CG^CG
94 CGCG
EarI 187 GAAGAG CTCTTC(1/4)
889 GAAGAG
HphI 124 GGTGA GGTGA(8/7)
286 GGTGA
Ksp632I 187 GAAGAG CTCTTC(1/4)
889 GAAGAG
MseI 443 TTAA T^TAA
587 TTAA
MvnI 92 CGCG CG^CG
94 CGCG
NciI 296 CCCGG CC^[GC]GG
297 CCGGG
NdeI 756 CATATG CA^TATG
884 CATATG
PleI 289 GACTC GAGTC(4/5)
507 GACTC
PstI 359 CTGCAG CTGCA^G
452 CTGCAG
SfaNI 307 GATGC GCATC(5/9)
1009 GATGC
TaqI 56 TCGA T^CGA
644 TCGA
Tru9I 443 TTAA T^TAA
587 TTAA
TspRI 212 CAGTG CA[GC]TG[ACGT][ACGT]^
677 CACTG
TthHB8I 56 TCGA T^CGA
644 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BssKI BstF5I BstYI Cac8I
CfoI DdeI DpnII Fnu4HI FokI
HhaI HinP1I HinfI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI SfcI TfiI TseI Tsp45I
Tsp509I XhoII