Restriction map for
pF1KB3651
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BlnI BsaAI BsaHI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI SwaI Tth111I
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 2554 CGCG CG^CG
AcsI 2881 GAATTT [GA]^AATT[CT]
AflIII 1806 ACATGT A^C[GA][CT]GT
AocI 887 CCTCAGG CC^T[ACGT]AGG
ApaI 2964 GGGCCC GGGCC^C
ApoI 2881 GAATTT [GA]^AATT[CT]
AvaI 1664 CCCGGG C^[CT]CG[GA]G
BanI 567 GGTGCC G^G[CT][GA]CC
BcgI 2838 CGAGTTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2978 AGATCT A^GATCT
BlpI 1100 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1100 GCTCAGC GC^T[ACGT]AGC
BsaI 3007 GAGACC GGTCTC(1/5)
BsaWI 2766 ACCGGA [AT]^CCGG[AT]
BsiEI 326 CGGCCG CG[GA][CT]^CG
BsmI 2067 GAATGC GAATGC(1/-1)
BsoBI 1664 CCCGGG C^[CT]CG[GA]G
BspLU11I 1806 ACATGT A^CATGT
BsrDI 2592 CATTGC GCAATG(2/0)
BsrFI 1226 ACCGGC [GA]^CCGG[CT]
BstUI 2554 CGCG CG^CG
BstXI 1728 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 887 CCTCAGG CC^T[ACGT]AGG
CelII 1100 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1226 ACCGGC [GA]^CCGG[CT]
DraIII 860 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 326 CGGCCG C^GGCCG
EclXI 326 CGGCCG C^GGCCG
Eco52I 326 CGGCCG C^GGCCG
Eco81I 887 CCTCAGG CC^T[ACGT]AGG
MaeII 2730 ACGT A^CGT
MfeI 230 CAATTG C^AATTG
MseI 2924 TTAA T^TAA
MunI 230 CAATTG C^AATTG
MvnI 2554 CGCG CG^CG
NspI 1806 ACATGT [GA]CATG^[CT]
SacI 360 GAGCTC GAGCT^C
SapI 2025 GAAGAGC GCTCTTC(1/4)
ScaI 2402 AGTACT AGT^ACT
SfiI 1698 GGCCCATTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1664 CCCGGG CCC^GGG
SpeI 2091 ACTAGT A^CTAGT
Tru9I 2924 TTAA T^TAA
XbaI 2146 TCTAGA T^CTAGA
XmaCI 1664 CCCGGG C^CCGGG
XmaI 1664 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Alw44I 1739 GTGCAC G^TGCAC
1882 GTGCAC
ApaLI 1739 GTGCAC G^TGCAC
1882 GTGCAC
BalI 314 TGGCCA TGG^CCA
1032 TGGCCA
BanII 360 GAGCTC G[GA]GC[CT]^C
2964 GGGCCC
BbsI 2635 GAAGAC GAAGAC(2/6)
1434 GTCTTC
BbvCI 644 CCTCAGC CCTCAGC(-5/-2)
1342 CCTCAGC
BglI 1006 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1699 GCCCATTTGGC
BpuAI 2635 GAAGAC GAAGAC(2/6)
1434 GTCTTC
BsaBI 601 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1194 GATTGCCATC
DsaI 833 CCATGG C^C[GA][CT]GG
2138 CCATGG
MamI 601 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1194 GATTGCCATC
MluNI 314 TGGCCA TGG^CCA
1032 TGGCCA
MscI 314 TGGCCA TGG^CCA
1032 TGGCCA
MslI 525 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
595 CATGCAGATG
NcoI 833 CCATGG C^CATGG
2138 CCATGG
TaqI 304 TCGA T^CGA
1596 TCGA
TthHB8I 304 TCGA T^CGA
1596 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XhoII