Restriction map for
pF1KB3657
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I Cac8I CelII
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT14I FseI
FspI HpaI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 844 GTCGAC GT^[AC][GT]AC
AcyI 97 GGCGCC G[GA]^CG[CT]C
AflIII 144 ACGTGT A^C[GA][CT]GT
AvaI 61 CCCGAG C^[CT]CG[GA]G
BalI 548 TGGCCA TGG^CCA
BbeI 97 GGCGCC GGCGC^C
BbsI 478 GAAGAC GAAGAC(2/6)
BbvI 118 GCTGC GCAGC(8/12)
BclI 969 TGATCA T^GATCA
BglII 333 AGATCT A^GATCT
BpuAI 478 GAAGAC GAAGAC(2/6)
BsaBI 301 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsaI 281 GGTCTC GGTCTC(1/5)
BsoBI 61 CCCGAG C^[CT]CG[GA]G
BspMI 273 ACCTGC ACCTGC(4/8)
BsrFI 26 ACCGGC [GA]^CCGG[CT]
BstAPI 699 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 312 GGTGACC G^GT[ACGT]ACC
BstPI 312 GGTGACC G^GT[ACGT]ACC
BstXI 830 CCAATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 333 AGATCT [GA]^GATC[CT]
CfoI 98 GCGC GCG^C
Cfr10I 26 ACCGGC [GA]^CCGG[CT]
DraIII 552 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1066 GAAGAG CTCTTC(1/4)
EcoO65I 312 GGTGACC G^GT[ACGT]ACC
EcoRI 292 GAATTC G^AATTC
FauI 727 CCCGC CCCGC(4/6)
FbaI 969 TGATCA T^GATCA
HaeII 97 GGCGCC [GA]GCGC^[CT]
HapII 27 CCGG C^CGG
HgaI 708 GCGTC GACGC(5/10)
HhaI 98 GCGC GCG^C
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HinP1I 98 GCGC G^CGC
HindIII 361 AAGCTT A^AGCTT
HpaII 27 CCGG C^CGG
HphI 312 GGTGA GGTGA(8/7)
KasI 97 GGCGCC G^GCGCC
Ksp632I 1066 GAAGAG CTCTTC(1/4)
MamI 301 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 333 AGATCT [GA]^GATC[CT]
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MspI 27 CCGG C^CGG
NarI 97 GGCGCC GG^CGCC
NdeI 229 CATATG CA^TATG
NspI 912 ACATGC [GA]CATG^[CT]
PvuII 428 CAGCTG CAG^CTG
SalI 844 GTCGAC G^TCGAC
SfcI 204 CTACAG C^T[GA][CT]AG
SfoI 97 GGCGCC GGC^GCC
TaqI 845 TCGA T^CGA
TfiI 495 GATTC G^A[AT]TC
TseI 118 GCTGC G^C[AT]GC
Tsp45I 313 GTGAC ^GT[GC]AC
TthHB8I 845 TCGA T^CGA
XhoII 333 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 292 GAATTC [GA]^AATT[CT]
398 AAATTC
AlwI 937 GATCC GGATC(4/5)
961 GATCC
AlwNI 31 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
73 CAGAACCTG
ApoI 292 GAATTC [GA]^AATT[CT]
398 AAATTC
AvaII 447 GGACC G^G[AT]CC
990 GGACC
BanII 90 GGGCTC G[GA]GC[CT]^C
566 GGGCTC
BsaAI 143 TACGTG [CT]AC^GT[GA]
544 TACGTG
BsaJI 405 CCGAGG C^C[ACGT][ACGT]GG
836 CCCTGG
BsiYI 318 CCATCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
551 CCACTAGGTGG
BslI 318 CCATCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
551 CCACTAGGTGG
BsmFI 446 GGGAC GGGAC(10/14)
989 GGGAC
BssKI 670 CCTGG ^CC[ACGT]GG
837 CCTGG
BstNI 670 CCTGG CC^[AT]GG
837 CCTGG
DraII 446 GGGACCT [GA]G^G[ACGT]CC[CT]
989 GGGACCT
EcoO109I 446 GGGACCT [GA]G^G[ACGT]CC[CT]
989 GGGACCT
EcoRII 670 CCTGG ^CC[AT]GG
837 CCTGG
EcoT22I 592 ATGCAT ATGCA^T
630 ATGCAT
Fnu4HI 118 GCTGC GC^[ACGT]GC
895 GCGGC
HincII 648 GTTGAC GT[CT]^[GA]AC
844 GTCGAC
HindII 648 GTTGAC GT[CT]^[GA]AC
844 GTCGAC
ItaI 118 GCTGC GC^[ACGT]GC
895 GCGGC
MslI 238 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
520 CACACAGATG
MspA1I 428 CAGCTG C[AC]G^C[GT]G
893 CAGCGG
MvaI 670 CCTGG CC^[AT]GG
837 CCTGG
NsiI 592 ATGCAT ATGCA^T
630 ATGCAT
PflMI 318 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCACTAGGTGG
PpuMI 446 GGGACCT [GA]G^G[AT]CC[CT]
989 GGGACCT
ScrFI 670 CCTGG CC^[ACGT]GG
837 CCTGG
Van91I 318 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
551 CCACTAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BanI BfaI
BmyI BpmI BsmAI Bsp1286I BsrI
BstF5I Cfr13I DdeI DpnII EaeI
FokI HaeIII HinfI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I SfaNI Tru9I
Tsp509I TspRI