Restriction map for
pF1KB3680
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HaeII HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 2813 GACGCC G[GA]^CG[CT]C
AocI 2152 CCTAAGG CC^T[ACGT]AGG
AspI 2752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 649 CCCGGG C^[CT]CG[GA]G
BamHI 1257 GGATCC G^GATCC
BglII 1401 AGATCT A^GATCT
BsaHI 2813 GACGCC G[GA]^CG[CT]C
BsaI 217 GAGACC GGTCTC(1/5)
BsmI 2562 GAATGC GAATGC(1/-1)
BsoBI 649 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2573 TGTACA T^GTACA
BspMI 1752 ACCTGC ACCTGC(4/8)
BsrBI 1615 GAGCGG CCGCTC(-3/-3)
BsrGI 2573 TGTACA T^GTACA
Bsu36I 2152 CCTAAGG CC^T[ACGT]AGG
CfoI 1159 GCGC GCG^C
DraII 932 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 2587 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2152 CCTAAGG CC^T[ACGT]AGG
EcoNI 915 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 932 GGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 2813 GACGC GACGC(5/10)
HhaI 1159 GCGC GCG^C
Hin1I 2813 GACGCC G[GA]^CG[CT]C
HinP1I 1159 GCGC G^CGC
NspI 1964 GCATGT [GA]CATG^[CT]
PflFI 2752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1975 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 932 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 817 GAGCTC GAGCT^C
SapI 1444 GAAGAGC GCTCTTC(1/4)
SmaI 649 CCCGGG CCC^GGG
SspBI 2573 TGTACA T^GTACA
StuI 2467 AGGCCT AGG^CCT
Tth111I 2752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1975 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1241 CCATGGAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 649 CCCGGG C^CCGGG
XmaI 649 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcsI 2348 AAATTT [GA]^AATT[CT]
2530 GAATTT
ApoI 2348 AAATTT [GA]^AATT[CT]
2530 GAATTT
Asp700I 379 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2329 GAAGCTATTC
AspHI 817 GAGCTC G[AT]GC[AT]^C
1007 GTGCTC
BalI 891 TGGCCA TGG^CCA
2654 TGGCCA
BclI 1304 TGATCA T^GATCA
1503 TGATCA
BfaI 848 CTAG C^TAG
2143 CTAG
BlpI 30 GCTAAGC GC^T[ACGT]AGC
304 GCTGAGC
Bpu1102I 30 GCTAAGC GC^T[ACGT]AGC
304 GCTGAGC
BsaBI 1501 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2593 GATGTCTATC
BsiHKAI 817 GAGCTC G[AT]GC[AT]^C
1007 GTGCTC
BspHI 92 TCATGA T^CATGA
1148 TCATGA
BstAPI 1000 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1894 GCAGATGTTGC
BstEII 536 GGTTACC G^GT[ACGT]ACC
642 GGTAACC
BstPI 536 GGTTACC G^GT[ACGT]ACC
642 GGTAACC
BstXI 542 CCACTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1811 CCACAAGTATGG
CelII 30 GCTAAGC GC^T[ACGT]AGC
304 GCTGAGC
EcoO65I 536 GGTTACC G^GT[ACGT]ACC
642 GGTAACC
FbaI 1304 TGATCA T^GATCA
1503 TGATCA
HincII 1220 GTCAAC GT[CT]^[GA]AC
2481 GTCAAC
HindII 1220 GTCAAC GT[CT]^[GA]AC
2481 GTCAAC
MaeI 848 CTAG C^TAG
2143 CTAG
MamI 1501 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2593 GATGTCTATC
MluNI 891 TGGCCA TGG^CCA
2654 TGGCCA
MscI 891 TGGCCA TGG^CCA
2654 TGGCCA
MseI 1100 TTAA T^TAA
2489 TTAA
PvuII 1874 CAGCTG CAG^CTG
2525 CAGCTG
RcaI 92 TCATGA T^CATGA
1148 TCATGA
ScaI 200 AGTACT AGT^ACT
1867 AGTACT
TaqI 1335 TCGA T^CGA
2709 TCGA
Tru9I 1100 TTAA T^TAA
2489 TTAA
TthHB8I 1335 TCGA T^CGA
2709 TCGA
XmnI 379 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2329 GAAGCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XhoII