Restriction map for pF1KB3682
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Aor51HI     
ApaI        AscI        Asp718I     AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MflI        MluI        MluNI       
MroI        MscI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       Sse8387I    SspBI       StuI        SwaI        
Tth111I     XhoII       


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AflIII       1565  ACATGT               A^C[GA][CT]GT
Alw44I       1481  GTGCAC               G^TGCAC
AlwI          774  GGATC                GGATC(4/5)
AlwNI         100  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2188  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1481  GTGCAC               G^TGCAC
AseI          700  ATTAAT               AT^TAAT
AsnI          700  ATTAAT               AT^TAAT
BciVI        2007  GTATCC               GTATCC(6/5)
BlpI         1492  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1492  GCTCAGC              GC^T[ACGT]AGC
BsmBI        1351  GAGACG               CGTCTC(1/5)
BsmI          690  GAATGC               GAATGC(1/-1)
BspHI         177  TCATGA               T^CATGA
BspLU11I     1565  ACATGT               A^CATGT
BssSI         550  CACGAG               CACGAG(-5/-1)
Bsu36I       2188  CCTGAGG              CC^T[ACGT]AGG
CelII        1492  GCTCAGC              GC^T[ACGT]AGC
CfoI          740  GCGC                 GCG^C
DraIII       1475  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          433  GACAGTAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1288  GAAGAG               CTCTTC(1/4)
Eco81I       2188  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2230  GAATTC               G^AATTC
EcoT14I        86  CCATGG               C^C[AT][AT]GG
HaeII         739  AGCGCC               [GA]GCGC^[CT]
HhaI          740  GCGC                 GCG^C
HinP1I        740  GCGC                 G^CGC
HindIII      1579  AAGCTT               A^AGCTT
Ksp632I      1288  GAAGAG               CTCTTC(1/4)
MaeII        1385  ACGT                 A^CGT
NcoI           86  CCATGG               C^CATGG
PaeR7I       1348  CTCGAG               C^TCGAG
PflMI        1415  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1014  GACAGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         700  ATTAAT               AT^TAAT
RcaI          177  TCATGA               T^CATGA
SapI         1288  GAAGAGC              GCTCTTC(1/4)
SmaI         1826  CCCGGG               CCC^GGG
SpeI          711  ACTAGT               A^CTAGT
SphI          977  GCATGC               GCATG^C
StyI           86  CCATGG               C^C[AT][AT]GG
Van91I       1415  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2149  TCTAGA               T^CTAGA
XhoI         1348  CTCGAG               C^TCGAG
XmaCI        1826  CCCGGG               C^CCGGG
XmaI         1826  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AhdI          181  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2200  GACGAGGTGTC         
AspEI         181  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2200  GACGAGGTGTC         
BanI         1220  GGCACC               G^G[CT][GA]CC
             1252  GGCACC              
BbsI          292  GAAGAC               GAAGAC(2/6)
             1011  GAAGAC              
BcgI         1355  CGACCTCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              816  GCAGCAAGTTCG        
BpmI         2049  CTCCAG               CTGGAG(16/14)
               49  CTGGAG              
BpuAI         292  GAAGAC               GAAGAC(2/6)
             1011  GAAGAC              
BseRI        1183  GAGGAG               GAGGAG(10/8)
             2191  GAGGAG              
BsrBI        1401  GAGCGG               CCGCTC(-3/-3)
             1858  GAGCGG              
BstXI         557  CCAATGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2028  CCATGCCTTTGG        
DraII        1269  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1795  GGGACCT             
DsaI           86  CCATGG               C^C[GA][CT]GG
             1618  CCGTGG              
Eam1105I      181  GACCTCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2200  GACGAGGTGTC         
EcoNI          48  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1773  CCTGTTGAAGG         
EcoO109I     1269  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1795  GGGACCT             
HapII        1792  CCGG                 C^CGG
             1827  CCGG                
HpaII        1792  CCGG                 C^CGG
             1827  CCGG                
MfeI         1228  CAATTG               C^AATTG
             1318  CAATTG              
MspI         1792  CCGG                 C^CGG
             1827  CCGG                
MunI         1228  CAATTG               C^AATTG
             1318  CAATTG              
PpuMI        1269  GGGTCCT              [GA]G^G[AT]CC[CT]
             1795  GGGACCT             
PstI          781  CTGCAG               CTGCA^G
              814  CTGCAG              
SacI          948  GAGCTC               GAGCT^C
             1821  GAGCTC              
SfaNI        1685  GCATC                GCATC(5/9)
             1736  GCATC               
SmlI          601  CTCAAG               C^T[CT][GA]AG
             1348  CTCGAG              
TaqI         1349  TCGA                 T^CGA
             2234  TCGA                
TfiI         1452  GAATC                G^A[AT]TC
             1989  GAATC               
TthHB8I      1349  TCGA                 T^CGA
             2234  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BanII       
BbvI        BclI        BcnI        BfaI        BmyI        
BsaI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      FbaI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SspI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
XmnI        
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