Restriction map for
pF1KB3700
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbrPI BbvCI BciVI BfrI
BglII BlnI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspEI BspHI BssHII Bst1107I BstXI
BstYI BstZ17I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI MfeI MflI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI TfiI XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 1233 GTCTAC GT^[AC][GT]AC
AcsI 1768 GAATTT [GA]^AATT[CT]
AcyI 1081 GGCGCC G[GA]^CG[CT]C
AlwI 837 GATCC GGATC(4/5)
AocI 45 CCTGAGG CC^T[ACGT]AGG
ApoI 1768 GAATTT [GA]^AATT[CT]
BalI 1265 TGGCCA TGG^CCA
BbeI 1081 GGCGCC GGCGC^C
BcgI 253 GCAGCCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 484 CTAG C^TAG
BlpI 1716 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1716 GCTGAGC GC^T[ACGT]AGC
BsaBI 1206 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1081 GGCGCC G[GA]^CG[CT]C
Bsp1407I 1734 TGTACA T^GTACA
BspDI 406 ATCGAT AT^CGAT
BspLU11I 1563 ACATGT A^CATGT
BsrDI 464 GCAATG GCAATG(2/0)
BsrGI 1734 TGTACA T^GTACA
BssSI 6 CTCGTG CACGAG(-5/-1)
BstAPI 1317 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1174 TTCGAA TT^CGAA
BstEII 1551 GGTGACC G^GT[ACGT]ACC
BstPI 1551 GGTGACC G^GT[ACGT]ACC
Bsu36I 45 CCTGAGG CC^T[ACGT]AGG
CelII 1716 GCTGAGC GC^T[ACGT]AGC
ClaI 406 ATCGAT AT^CGAT
DraIII 1750 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 45 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1551 GGTGACC G^GT[ACGT]ACC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
Hin1I 1081 GGCGCC G[GA]^CG[CT]C
HincII 427 GTCAAC GT[CT]^[GA]AC
HindII 427 GTCAAC GT[CT]^[GA]AC
KasI 1081 GGCGCC G^GCGCC
KspI 1116 CCGCGG CCGC^GG
MaeI 484 CTAG C^TAG
MamI 1206 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1265 TGGCCA TGG^CCA
MscI 1265 TGGCCA TGG^CCA
MseI 655 TTAA T^TAA
NarI 1081 GGCGCC GG^CGCC
NspV 1174 TTCGAA TT^CGAA
PflMI 522 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1324 CTGCAG CTGCA^G
SacI 1524 GAGCTC GAGCT^C
SacII 1116 CCGCGG CCGC^GG
SapI 75 GCTCTTC GCTCTTC(1/4)
SfcI 1324 CTGCAG C^T[GA][CT]AG
SfoI 1081 GGCGCC GGC^GCC
SfuI 1174 TTCGAA TT^CGAA
SmaI 275 CCCGGG CCC^GGG
SmlI 1585 CTCAAG C^T[CT][GA]AG
SphI 1313 GCATGC GCATG^C
SspBI 1734 TGTACA T^GTACA
StuI 494 AGGCCT AGG^CCT
StyI 161 CCAAGG C^C[AT][AT]GG
Tru9I 655 TTAA T^TAA
Tsp509I 1769 AATT ^AATT
Van91I 522 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 275 CCCGGG C^CCGGG
XmaI 275 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 986 ACGTGT A^C[GA][CT]GT
1563 ACATGT
ApaI 136 GGGCCC GGGCC^C
877 GGGCCC
AspI 397 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1639 GACAAAGTC
AvaI 275 CCCGGG C^[CT]CG[GA]G
1390 CCCGAG
BanI 1081 GGCGCC G^G[CT][GA]CC
1666 GGCACC
BbsI 1369 GTCTTC GAAGAC(2/6)
1459 GTCTTC
BclI 1025 TGATCA T^GATCA
1619 TGATCA
BpuAI 1369 GTCTTC GAAGAC(2/6)
1459 GTCTTC
BsmAI 622 GAGAC GTCTC(1/5)
1637 GAGAC
BsoBI 275 CCCGGG C^[CT]CG[GA]G
1390 CCCGAG
DsaI 795 CCACGG C^C[GA][CT]GG
1116 CCGCGG
EarI 76 CTCTTC CTCTTC(1/4)
91 CTCTTC
FbaI 1025 TGATCA T^GATCA
1619 TGATCA
HaeII 377 GGCGCT [GA]GCGC^[CT]
1081 GGCGCC
Ksp632I 76 CTCTTC CTCTTC(1/4)
91 CTCTTC
MslI 768 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1464 CACTGATGTG
PflFI 397 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1639 GACAAAGTC
PvuII 710 CAGCTG CAG^CTG
1546 CAGCTG
Tth111I 397 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1639 GACAAAGTC
XcmI 522 CCAGGCCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1510 CCACACACCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwNI ApaLI AspHI AvaII AviII
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TspRI TthHB8I