Restriction map for
pF1KB3707
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AflII AflIII Aor51HI
AscI AseI AsnI AspI AviII
AvrII BbeI BbrPI BfrI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XcmI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1762 GGTACC G^GTACC
AccI 1324 GTCTAC GT^[AC][GT]AC
AccII 908 CGCG CG^CG
AclI 2565 AACGTT AA^CGTT
AcsI 1039 GAATTT [GA]^AATT[CT]
AcyI 2026 GGCGTC G[GA]^CG[CT]C
AgeI 204 ACCGGT A^CCGGT
AhdI 1597 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 400 GTGCAC G^TGCAC
ApaLI 400 GTGCAC G^TGCAC
ApoI 1039 GAATTT [GA]^AATT[CT]
Asp700I 1516 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1762 GGTACC G^GTACC
AspEI 1597 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2887 GGATCC G^GATCC
BcgI 567 GCAGGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1590 GTATCC GTATCC(6/5)
BclI 518 TGATCA T^GATCA
BsaHI 2026 GGCGTC G[GA]^CG[CT]C
BsaWI 204 ACCGGT [AT]^CCGG[AT]
BsmI 2604 GAATGC GAATGC(1/-1)
Bsp1407I 1086 TGTACA T^GTACA
BsrBI 1069 GAGCGG CCGCTC(-3/-3)
BsrFI 204 ACCGGT [GA]^CCGG[CT]
BsrGI 1086 TGTACA T^GTACA
BstUI 908 CGCG CG^CG
Cfr10I 204 ACCGGT [GA]^CCGG[CT]
DraI 1288 TTTAAA TTT^AAA
DraIII 83 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1597 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2827 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 518 TGATCA T^GATCA
HgaI 2027 GCGTC GACGC(5/10)
Hin1I 2026 GGCGTC G[GA]^CG[CT]C
KpnI 1762 GGTACC GGTAC^C
MvnI 908 CGCG CG^CG
NspI 2498 GCATGT [GA]CATG^[CT]
PinAI 204 ACCGGT A^CCGGT
Psp1406I 2565 AACGTT AA^CGTT
SgrAI 203 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2771 CCCGGG CCC^GGG
SspBI 1086 TGTACA T^GTACA
StuI 993 AGGCCT AGG^CCT
XbaI 1656 TCTAGA T^CTAGA
XmaCI 2771 CCCGGG C^CCGGG
XmaI 2771 CCCGGG C^CCGGG
XmnI 1516 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
ApaI 1420 GGGCCC GGGCC^C
2513 GGGCCC
BalI 140 TGGCCA TGG^CCA
917 TGGCCA
BanI 1074 GGTGCC G^G[CT][GA]CC
1762 GGTACC
BfaI 478 CTAG C^TAG
1657 CTAG
HaeII 551 AGCGCC [GA]GCGC^[CT]
2326 AGCGCC
MaeI 478 CTAG C^TAG
1657 CTAG
MaeII 291 ACGT A^CGT
2566 ACGT
MluNI 140 TGGCCA TGG^CCA
917 TGGCCA
MscI 140 TGGCCA TGG^CCA
917 TGGCCA
SacI 826 GAGCTC GAGCT^C
1861 GAGCTC
SmlI 1864 CTCAAG C^T[CT][GA]AG
2506 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII