Restriction map for
pF1KB3725
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII AccII AclI Aor51HI ApaI
AscI AviII AvrII BcgI BciVI
BglI BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI BspDI BspLU11I
BssHII BssSI BstEII BstPI BstUI
CelII ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI HpaI KspI MamI MluI
MvnI NaeI NgoMIV NheI NotI
NruI NspI PacI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1355 TCCGGA T^CCGGA
AcyI 370 GGCGCC G[GA]^CG[CT]C
AflII 3859 CTTAAG C^TTAAG
AflIII 4036 ACACGT A^C[GA][CT]GT
AgeI 3716 ACCGGT A^CCGGT
AhdI 2587 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2587 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2044 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3170 GGATCC G^GATCC
BbeI 370 GGCGCC GGCGC^C
BbvCI 3132 GCTGAGG CCTCAGC(-5/-2)
BfrI 3859 CTTAAG C^TTAAG
BglII 2456 AGATCT A^GATCT
BsaHI 370 GGCGCC G[GA]^CG[CT]C
BseAI 1355 TCCGGA T^CCGGA
BsgI 63 CTGCAC GTGCAG(16/14)
BspEI 1355 TCCGGA T^CCGGA
BsrBI 101 GAGCGG CCGCTC(-3/-3)
Bst1107I 1793 GTATAC GTA^TAC
BstBI 4109 TTCGAA TT^CGAA
BstXI 419 CCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1793 GTATAC GTA^TAC
Eam1105I 2587 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1635 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 967 GATATC GAT^ATC
EcoT22I 1064 ATGCAT ATGCA^T
HgaI 1454 GCGTC GACGC(5/10)
Hin1I 370 GGCGCC G[GA]^CG[CT]C
HincII 699 GTTGAC GT[CT]^[GA]AC
HindII 699 GTTGAC GT[CT]^[GA]AC
KasI 370 GGCGCC G^GCGCC
MfeI 2336 CAATTG C^AATTG
MroI 1355 TCCGGA T^CCGGA
MunI 2336 CAATTG C^AATTG
NarI 370 GGCGCC GG^CGCC
NdeI 281 CATATG CA^TATG
NsiI 1064 ATGCAT ATGCA^T
NspV 4109 TTCGAA TT^CGAA
PaeR7I 216 CTCGAG C^TCGAG
PflFI 2044 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3716 ACCGGT A^CCGGT
PpuMI 2267 AGGTCCC [GA]G^G[AT]CC[CT]
ScaI 809 AGTACT AGT^ACT
SfoI 370 GGCGCC GGC^GCC
SfuI 4109 TTCGAA TT^CGAA
Tth111I 2044 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 216 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
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Alw44I 615 GTGCAC G^TGCAC
4080 GTGCAC
AocI 3631 CCTCAGG CC^T[ACGT]AGG
3662 CCTCAGG
ApaLI 615 GTGCAC G^TGCAC
4080 GTGCAC
BbrPI 1768 CACGTG CAC^GTG
4037 CACGTG
BclI 287 TGATCA T^GATCA
3564 TGATCA
BsaAI 1768 CACGTG [CT]AC^GT[GA]
4037 CACGTG
BsaI 1235 GAGACC GGTCTC(1/5)
2594 GGTCTC
BsaWI 1355 TCCGGA [AT]^CCGG[AT]
3716 ACCGGT
BsmI 2737 GCATTC GAATGC(1/-1)
3059 GCATTC
BspHI 689 TCATGA T^CATGA
3696 TCATGA
BspMI 2086 GCAGGT ACCTGC(4/8)
2265 GCAGGT
BsrDI 2180 CATTGC GCAATG(2/0)
3273 CATTGC
Bsu36I 3631 CCTCAGG CC^T[ACGT]AGG
3662 CCTCAGG
CfoI 176 GCGC GCG^C
371 GCGC
DraII 332 AGGCCCT [GA]G^G[ACGT]CC[CT]
2267 AGGTCCC
DraIII 1045 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4083 CACACTGTG
EarI 1818 CTCTTC CTCTTC(1/4)
1963 GAAGAG
Eco81I 3631 CCTCAGG CC^T[ACGT]AGG
3662 CCTCAGG
EcoO109I 332 AGGCCCT [GA]G^G[ACGT]CC[CT]
2267 AGGTCCC
EcoRI 893 GAATTC G^AATTC
3038 GAATTC
FauI 254 GCGGG CCCGC(4/6)
348 GCGGG
FbaI 287 TGATCA T^GATCA
3564 TGATCA
HaeII 175 AGCGCC [GA]GCGC^[CT]
370 GGCGCC
HhaI 176 GCGC GCG^C
371 GCGC
HinP1I 176 GCGC G^CGC
371 GCGC
Ksp632I 1818 CTCTTC CTCTTC(1/4)
1963 GAAGAG
PflMI 2214 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2529 CCAGTATATGG
PmaCI 1768 CACGTG CAC^GTG
4037 CACGTG
PmlI 1768 CACGTG CAC^GTG
4037 CACGTG
RcaI 689 TCATGA T^CATGA
3696 TCATGA
SacI 706 GAGCTC GAGCT^C
1351 GAGCTC
SspI 1686 AATATT AAT^ATT
2206 AATATT
Van91I 2214 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2529 CCAGTATATGG
XcmI 3500 CCATTACCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3758 CCAATAGATTACTGG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI KpnI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI