Restriction map for
pF1KB3732
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbvCI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 730 AAATTC [GA]^AATT[CT]
AcyI 1051 GACGCC G[GA]^CG[CT]C
AflIII 367 ACGTGT A^C[GA][CT]GT
AlwNI 708 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 730 AAATTC [GA]^AATT[CT]
AspI 460 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1149 CCCGGG C^[CT]CG[GA]G
AvaII 210 GGACC G^G[AT]CC
BcgI 472 CGAAATCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 682 GCCCATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1278 AGATCT A^GATCT
BsaHI 1051 GACGCC G[GA]^CG[CT]C
BsgI 1318 CTGCAC GTGCAG(16/14)
BsmI 1333 GAATGC GAATGC(1/-1)
BsoBI 1149 CCCGGG C^[CT]CG[GA]G
BstXI 842 CCAGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1278 AGATCT [GA]^GATC[CT]
DdeI 300 CTTAG C^T[ACGT]AG
DraIII 1234 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 229 GACCAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 702 CCAAGG C^C[AT][AT]GG
EcoT22I 504 ATGCAT ATGCA^T
FauI 600 GCGGG CCCGC(4/6)
HgaI 1051 GACGC GACGC(5/10)
Hin1I 1051 GACGCC G[GA]^CG[CT]C
HincII 790 GTTGAC GT[CT]^[GA]AC
HindII 790 GTTGAC GT[CT]^[GA]AC
HinfI 3 GACTC G^A[ACGT]TC
MflI 1278 AGATCT [GA]^GATC[CT]
NsiI 504 ATGCAT ATGCA^T
NspI 1070 GCATGT [GA]CATG^[CT]
PflFI 460 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 775 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 3 GACTC GAGTC(4/5)
PstI 610 CTGCAG CTGCA^G
PvuII 1018 CAGCTG CAG^CTG
SfaNI 506 GCATC GCATC(5/9)
SfcI 610 CTGCAG C^T[GA][CT]AG
SmaI 1149 CCCGGG CCC^GGG
SmlI 240 CTCAAG C^T[CT][GA]AG
SspI 198 AATATT AAT^ATT
StyI 702 CCAAGG C^C[AT][AT]GG
TaqI 1376 TCGA T^CGA
Tth111I 460 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1376 TCGA T^CGA
Van91I 775 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1278 AGATCT [GA]^GATC[CT]
XmaCI 1149 CCCGGG C^CCGGG
XmaI 1149 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 470 CGCG CG^CG
946 CGCG
AfaI 285 GTAC GT^AC
1182 GTAC
AspHI 313 GAGCTC G[AT]GC[AT]^C
658 GAGCTC
BalI 557 TGGCCA TGG^CCA
1252 TGGCCA
BbrPI 366 CACGTG CAC^GTG
1237 CACGTG
BfaI 85 CTAG C^TAG
191 CTAG
BsaAI 366 CACGTG [CT]AC^GT[GA]
1237 CACGTG
BsiHKAI 313 GAGCTC G[AT]GC[AT]^C
658 GAGCTC
BsmFI 41 GGGAC GGGAC(10/14)
939 GTCCC
BstUI 470 CGCG CG^CG
946 CGCG
Cfr13I 210 GGACC G^G[ACGT]CC
1014 GGGCC
EaeI 557 TGGCCA [CT]^GGCC[GA]
1252 TGGCCA
EcoNI 1113 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1263 CCTGATGAAGG
MaeI 85 CTAG C^TAG
191 CTAG
MluNI 557 TGGCCA TGG^CCA
1252 TGGCCA
MscI 557 TGGCCA TGG^CCA
1252 TGGCCA
MseI 497 TTAA T^TAA
982 TTAA
MslI 214 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1323 CATGAAGGTG
MspA1I 357 CCGCTG C[AC]G^C[GT]G
1018 CAGCTG
MvnI 470 CGCG CG^CG
946 CGCG
NlaIV 1013 GGGGCC GG[ACGT]^[ACGT]CC
1294 GGAGCC
PmaCI 366 CACGTG CAC^GTG
1237 CACGTG
PmlI 366 CACGTG CAC^GTG
1237 CACGTG
RsaI 285 GTAC GT^AC
1182 GTAC
SacI 313 GAGCTC GAGCT^C
658 GAGCTC
SapI 117 GAAGAGC GCTCTTC(1/4)
604 GAAGAGC
Sau96I 210 GGACC G^G[ACGT]CC
1014 GGGCC
Tru9I 497 TTAA T^TAA
982 TTAA
XcmI 1017 CCAGCTGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1055 CCATCTTTGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII Sau3AI
ScrFI TseI Tsp45I Tsp509I TspRI