Restriction map for
pF1KB3767
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AviII AvrII BbeI
BbrPI BbvCI BcgI BfrI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 2594 GGTACC G^GTACC
AciI 2191 GCGG CCGC(-3/-1)
Asp718I 2594 GGTACC G^GTACC
AvaI 822 CTCGAG C^[CT]CG[GA]G
BalI 417 TGGCCA TGG^CCA
BamHI 2347 GGATCC G^GATCC
BciVI 171 GGATAC GTATCC(6/5)
BclI 2871 TGATCA T^GATCA
BcnI 139 CCGGG CC^[GC]GG
BsaBI 3139 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 118 GAGACC GGTCTC(1/5)
BsmBI 2647 GAGACG CGTCTC(1/5)
BsoBI 822 CTCGAG C^[CT]CG[GA]G
BstXI 2144 CCATTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 48 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2502 GAATTC G^AATTC
EcoRV 1225 GATATC GAT^ATC
FbaI 2871 TGATCA T^GATCA
HincII 1207 GTTAAC GT[CT]^[GA]AC
HindII 1207 GTTAAC GT[CT]^[GA]AC
HindIII 1430 AAGCTT A^AGCTT
HpaI 1207 GTTAAC GTT^AAC
KpnI 2594 GGTACC GGTAC^C
MamI 3139 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 417 TGGCCA TGG^CCA
MscI 417 TGGCCA TGG^CCA
MspA1I 1705 CAGCTG C[AC]G^C[GT]G
NciI 139 CCGGG CC^[GC]GG
PaeR7I 822 CTCGAG C^TCGAG
PshAI 261 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1705 CAGCTG CAG^CTG
SexAI 2597 ACCAGGT A^CC[AT]GGT
SpeI 3060 ACTAGT A^CTAGT
SspI 1870 AATATT AAT^ATT
XhoI 822 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 639 GTCTAC GT^[AC][GT]AC
1930 GTAGAC
AocI 685 CCTAAGG CC^T[ACGT]AGG
2160 CCTTAGG
Asp700I 143 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3199 GAAGAACTTC
AspHI 810 GAGCTC G[AT]GC[AT]^C
819 GAGCTC
AspI 2355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3266 GACAAAGTC
BanI 564 GGTGCC G^G[CT][GA]CC
2594 GGTACC
BanII 810 GAGCTC G[GA]GC[CT]^C
819 GAGCTC
BglII 1911 AGATCT A^GATCT
3213 AGATCT
BmyI 810 GAGCTC G[AGT]GC[ACT]^C
819 GAGCTC
BsgI 1708 CTGCAC GTGCAG(16/14)
2444 GTGCAG
BsiHKAI 810 GAGCTC G[AT]GC[AT]^C
819 GAGCTC
BsmFI 2156 GGGAC GGGAC(10/14)
51 GTCCC
Bsp1286I 810 GAGCTC G[AGT]GC[ACT]^C
819 GAGCTC
Bsp1407I 990 TGTACA T^GTACA
3236 TGTACA
BspHI 789 TCATGA T^CATGA
2868 TCATGA
BspMI 1296 ACCTGC ACCTGC(4/8)
496 GCAGGT
BsrDI 2171 CATTGC GCAATG(2/0)
2309 CATTGC
BsrGI 990 TGTACA T^GTACA
3236 TGTACA
Bsu36I 685 CCTAAGG CC^T[ACGT]AGG
2160 CCTTAGG
DsaI 1889 CCATGG C^C[GA][CT]GG
2489 CCATGG
EaeI 417 TGGCCA [CT]^GGCC[GA]
1917 TGGCCG
Eco81I 685 CCTAAGG CC^T[ACGT]AGG
2160 CCTTAGG
EcoT14I 1889 CCATGG C^C[AT][AT]GG
2489 CCATGG
HapII 139 CCGG C^CGG
1008 CCGG
HpaII 139 CCGG C^CGG
1008 CCGG
MaeII 637 ACGT A^CGT
2442 ACGT
MaeIII 2496 GTCAC ^GT[ACGT]AC
2796 GTCAC
MspI 139 CCGG C^CGG
1008 CCGG
NcoI 1889 CCATGG C^CATGG
2489 CCATGG
PflFI 2355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3266 GACAAAGTC
PpuMI 1826 AGGTCCT [GA]G^G[AT]CC[CT]
2156 GGGACCT
RcaI 789 TCATGA T^CATGA
2868 TCATGA
SacI 810 GAGCTC GAGCT^C
819 GAGCTC
SspBI 990 TGTACA T^GTACA
3236 TGTACA
StyI 1889 CCATGG C^C[AT][AT]GG
2489 CCATGG
Tsp45I 2496 GTCAC ^GT[GC]AC
2796 GTCAC
Tth111I 2355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3266 GACAAAGTC
XbaI 1422 TCTAGA T^CTAGA
3027 TCTAGA
XmnI 143 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3199 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbsI BbvI BfaI
BlpI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII