################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 2594 GGTACC G^GTACC AciI 2191 GCGG CCGC(-3/-1) Asp718I 2594 GGTACC G^GTACC AvaI 822 CTCGAG C^[CT]CG[GA]G BalI 417 TGGCCA TGG^CCA BamHI 2347 GGATCC G^GATCC BciVI 171 GGATAC GTATCC(6/5) BclI 2871 TGATCA T^GATCA BcnI 139 CCGGG CC^[GC]GG BsaBI 3139 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 118 GAGACC GGTCTC(1/5) BsmBI 2647 GAGACG CGTCTC(1/5) BsoBI 822 CTCGAG C^[CT]CG[GA]G BstXI 2144 CCATTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoNI 48 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2502 GAATTC G^AATTC EcoRV 1225 GATATC GAT^ATC FbaI 2871 TGATCA T^GATCA HincII 1207 GTTAAC GT[CT]^[GA]AC HindII 1207 GTTAAC GT[CT]^[GA]AC HindIII 1430 AAGCTT A^AGCTT HpaI 1207 GTTAAC GTT^AAC KpnI 2594 GGTACC GGTAC^C MamI 3139 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 417 TGGCCA TGG^CCA MscI 417 TGGCCA TGG^CCA MspA1I 1705 CAGCTG C[AC]G^C[GT]G NciI 139 CCGGG CC^[GC]GG PaeR7I 822 CTCGAG C^TCGAG PshAI 261 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1705 CAGCTG CAG^CTG SexAI 2597 ACCAGGT A^CC[AT]GGT SpeI 3060 ACTAGT A^CTAGT SspI 1870 AATATT AAT^ATT XhoI 822 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 639 GTCTAC GT^[AC][GT]AC 1930 GTAGAC AocI 685 CCTAAGG CC^T[ACGT]AGG 2160 CCTTAGG Asp700I 143 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3199 GAAGAACTTC AspHI 810 GAGCTC G[AT]GC[AT]^C 819 GAGCTC AspI 2355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3266 GACAAAGTC BanI 564 GGTGCC G^G[CT][GA]CC 2594 GGTACC BanII 810 GAGCTC G[GA]GC[CT]^C 819 GAGCTC BglII 1911 AGATCT A^GATCT 3213 AGATCT BmyI 810 GAGCTC G[AGT]GC[ACT]^C 819 GAGCTC BsgI 1708 CTGCAC GTGCAG(16/14) 2444 GTGCAG BsiHKAI 810 GAGCTC G[AT]GC[AT]^C 819 GAGCTC BsmFI 2156 GGGAC GGGAC(10/14) 51 GTCCC Bsp1286I 810 GAGCTC G[AGT]GC[ACT]^C 819 GAGCTC Bsp1407I 990 TGTACA T^GTACA 3236 TGTACA BspHI 789 TCATGA T^CATGA 2868 TCATGA BspMI 1296 ACCTGC ACCTGC(4/8) 496 GCAGGT BsrDI 2171 CATTGC GCAATG(2/0) 2309 CATTGC BsrGI 990 TGTACA T^GTACA 3236 TGTACA Bsu36I 685 CCTAAGG CC^T[ACGT]AGG 2160 CCTTAGG DsaI 1889 CCATGG C^C[GA][CT]GG 2489 CCATGG EaeI 417 TGGCCA [CT]^GGCC[GA] 1917 TGGCCG Eco81I 685 CCTAAGG CC^T[ACGT]AGG 2160 CCTTAGG EcoT14I 1889 CCATGG C^C[AT][AT]GG 2489 CCATGG HapII 139 CCGG C^CGG 1008 CCGG HpaII 139 CCGG C^CGG 1008 CCGG MaeII 637 ACGT A^CGT 2442 ACGT MaeIII 2496 GTCAC ^GT[ACGT]AC 2796 GTCAC MspI 139 CCGG C^CGG 1008 CCGG NcoI 1889 CCATGG C^CATGG 2489 CCATGG PflFI 2355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3266 GACAAAGTC PpuMI 1826 AGGTCCT [GA]G^G[AT]CC[CT] 2156 GGGACCT RcaI 789 TCATGA T^CATGA 2868 TCATGA SacI 810 GAGCTC GAGCT^C 819 GAGCTC SspBI 990 TGTACA T^GTACA 3236 TGTACA StyI 1889 CCATGG C^C[AT][AT]GG 2489 CCATGG Tsp45I 2496 GTCAC ^GT[GC]AC 2796 GTCAC Tth111I 2355 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3266 GACAAAGTC XbaI 1422 TCTAGA T^CTAGA 3027 TCTAGA XmnI 143 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3199 GAAGAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AvaII BbsI BbvI BfaI BlpI BpmI Bpu1102I BpuAI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII