Restriction map for pF1KB3767
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BfrI        BglI        
BlnI        BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      KasI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    StuI        
SwaI        Van91I      XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I       2594  GGTACC               G^GTACC
AciI         2191  GCGG                 CCGC(-3/-1)
Asp718I      2594  GGTACC               G^GTACC
AvaI          822  CTCGAG               C^[CT]CG[GA]G
BalI          417  TGGCCA               TGG^CCA
BamHI        2347  GGATCC               G^GATCC
BciVI         171  GGATAC               GTATCC(6/5)
BclI         2871  TGATCA               T^GATCA
BcnI          139  CCGGG                CC^[GC]GG
BsaBI        3139  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          118  GAGACC               GGTCTC(1/5)
BsmBI        2647  GAGACG               CGTCTC(1/5)
BsoBI         822  CTCGAG               C^[CT]CG[GA]G
BstXI        2144  CCATTCAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI          48  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2502  GAATTC               G^AATTC
EcoRV        1225  GATATC               GAT^ATC
FbaI         2871  TGATCA               T^GATCA
HincII       1207  GTTAAC               GT[CT]^[GA]AC
HindII       1207  GTTAAC               GT[CT]^[GA]AC
HindIII      1430  AAGCTT               A^AGCTT
HpaI         1207  GTTAAC               GTT^AAC
KpnI         2594  GGTACC               GGTAC^C
MamI         3139  GATTTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         417  TGGCCA               TGG^CCA
MscI          417  TGGCCA               TGG^CCA
MspA1I       1705  CAGCTG               C[AC]G^C[GT]G
NciI          139  CCGGG                CC^[GC]GG
PaeR7I        822  CTCGAG               C^TCGAG
PshAI         261  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1705  CAGCTG               CAG^CTG
SexAI        2597  ACCAGGT              A^CC[AT]GGT
SpeI         3060  ACTAGT               A^CTAGT
SspI         1870  AATATT               AAT^ATT
XhoI          822  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccI          639  GTCTAC               GT^[AC][GT]AC
             1930  GTAGAC              
AocI          685  CCTAAGG              CC^T[ACGT]AGG
             2160  CCTTAGG             
Asp700I       143  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3199  GAAGAACTTC          
AspHI         810  GAGCTC               G[AT]GC[AT]^C
              819  GAGCTC              
AspI         2355  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3266  GACAAAGTC           
BanI          564  GGTGCC               G^G[CT][GA]CC
             2594  GGTACC              
BanII         810  GAGCTC               G[GA]GC[CT]^C
              819  GAGCTC              
BglII        1911  AGATCT               A^GATCT
             3213  AGATCT              
BmyI          810  GAGCTC               G[AGT]GC[ACT]^C
              819  GAGCTC              
BsgI         1708  CTGCAC               GTGCAG(16/14)
             2444  GTGCAG              
BsiHKAI       810  GAGCTC               G[AT]GC[AT]^C
              819  GAGCTC              
BsmFI        2156  GGGAC                GGGAC(10/14)
               51  GTCCC               
Bsp1286I      810  GAGCTC               G[AGT]GC[ACT]^C
              819  GAGCTC              
Bsp1407I      990  TGTACA               T^GTACA
             3236  TGTACA              
BspHI         789  TCATGA               T^CATGA
             2868  TCATGA              
BspMI        1296  ACCTGC               ACCTGC(4/8)
              496  GCAGGT              
BsrDI        2171  CATTGC               GCAATG(2/0)
             2309  CATTGC              
BsrGI         990  TGTACA               T^GTACA
             3236  TGTACA              
Bsu36I        685  CCTAAGG              CC^T[ACGT]AGG
             2160  CCTTAGG             
DsaI         1889  CCATGG               C^C[GA][CT]GG
             2489  CCATGG              
EaeI          417  TGGCCA               [CT]^GGCC[GA]
             1917  TGGCCG              
Eco81I        685  CCTAAGG              CC^T[ACGT]AGG
             2160  CCTTAGG             
EcoT14I      1889  CCATGG               C^C[AT][AT]GG
             2489  CCATGG              
HapII         139  CCGG                 C^CGG
             1008  CCGG                
HpaII         139  CCGG                 C^CGG
             1008  CCGG                
MaeII         637  ACGT                 A^CGT
             2442  ACGT                
MaeIII       2496  GTCAC                ^GT[ACGT]AC
             2796  GTCAC               
MspI          139  CCGG                 C^CGG
             1008  CCGG                
NcoI         1889  CCATGG               C^CATGG
             2489  CCATGG              
PflFI        2355  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3266  GACAAAGTC           
PpuMI        1826  AGGTCCT              [GA]G^G[AT]CC[CT]
             2156  GGGACCT             
RcaI          789  TCATGA               T^CATGA
             2868  TCATGA              
SacI          810  GAGCTC               GAGCT^C
              819  GAGCTC              
SspBI         990  TGTACA               T^GTACA
             3236  TGTACA              
StyI         1889  CCATGG               C^C[AT][AT]GG
             2489  CCATGG              
Tsp45I       2496  GTCAC                ^GT[GC]AC
             2796  GTCAC               
Tth111I      2355  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3266  GACAAAGTC           
XbaI         1422  TCTAGA               T^CTAGA
             3027  TCTAGA              
XmnI          143  GAATAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3199  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AvaII       BbsI        BbvI        BfaI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MboI        MboII       MflI        MnlI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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