################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AvrII BbeI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrGI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI HaeII Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 945 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 168 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 83 CCCGAG C^[CT]CG[GA]G BamHI 158 GGATCC G^GATCC BbvCI 2281 CCTCAGC CCTCAGC(-5/-2) BclI 236 TGATCA T^GATCA BfaI 2339 CTAG C^TAG BglI 1891 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 1159 GAGGAG GAGGAG(10/8) BsoBI 83 CCCGAG C^[CT]CG[GA]G BspLU11I 360 ACATGT A^CATGT BssHII 7 GCGCGC G^CGCGC BstEII 1995 GGTGACC G^GT[ACGT]ACC BstPI 1995 GGTGACC G^GT[ACGT]ACC EcoNI 2468 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1995 GGTGACC G^GT[ACGT]ACC EcoRI 1988 GAATTC G^AATTC FbaI 236 TGATCA T^GATCA HindIII 730 AAGCTT A^AGCTT MaeI 2339 CTAG C^TAG NaeI 1622 GCCGGC GCC^GGC NgoMIV 1622 GCCGGC G^CCGGC PvuII 1273 CAGCTG CAG^CTG SbfI 1282 CCTGCAGG CCTGCA^GG SexAI 1467 ACCAGGT A^CC[AT]GGT Sse8387I 1282 CCTGCAGG CCTGCA^GG StuI 2172 AGGCCT AGG^CCT XmnI 168 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 1007 GGTACC G^GTACC 1636 GGTACC AcsI 706 GAATTT [GA]^AATT[CT] 1988 GAATTC Alw44I 825 GTGCAC G^TGCAC 2333 GTGCAC ApaLI 825 GTGCAC G^TGCAC 2333 GTGCAC ApoI 706 GAATTT [GA]^AATT[CT] 1988 GAATTC Asp718I 1007 GGTACC G^GTACC 1636 GGTACC AviII 759 TGCGCA TGC^GCA 1243 TGCGCA BalI 2441 TGGCCA TGG^CCA 2471 TGGCCA BanII 2324 GGGCTC G[GA]GC[CT]^C 2500 GGGCTC BsrFI 55 GCCGGT [GA]^CCGG[CT] 1622 GCCGGC BssSI 1438 CACGAG CACGAG(-5/-1) 1825 CACGAG Cfr10I 55 GCCGGT [GA]^CCGG[CT] 1622 GCCGGC FspI 759 TGCGCA TGC^GCA 1243 TGCGCA HincII 2245 GTTGAC GT[CT]^[GA]AC 2292 GTCAAC HindII 2245 GTTGAC GT[CT]^[GA]AC 2292 GTCAAC KpnI 1007 GGTACC GGTAC^C 1636 GGTACC MluNI 2441 TGGCCA TGG^CCA 2471 TGGCCA MscI 2441 TGGCCA TGG^CCA 2471 TGGCCA NcoI 354 CCATGG C^CATGG 1019 CCATGG PflMI 1627 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2122 CCACCCCGTGG SapI 110 GAAGAGC GCTCTTC(1/4) 168 GAAGAGC SspI 205 AATATT AAT^ATT 1804 AATATT Van91I 1627 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2122 CCACCCCGTGG XcmI 794 CCACCATCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1982 CCATAGGAATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AspHI AvaII BanI BbrPI BbsI BbvI BcnI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII