Restriction map for
pF1KB3797
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AgeI AhdI AlwI AlwNI
Aor51HI ApoI AscI Asp700I AspEI
AspI AvrII BamHI BbeI BbrPI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
Cfr10I CpoI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HpaI KasI Ksp632I
KspI MaeII MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 497 GGTACC G^GTACC
AccII 1294 CGCG CG^CG
AflII 712 CTTAAG C^TTAAG
Alw44I 388 GTGCAC G^TGCAC
ApaI 1000 GGGCCC GGGCC^C
ApaLI 388 GTGCAC G^TGCAC
AseI 796 ATTAAT AT^TAAT
AsnI 796 ATTAAT AT^TAAT
Asp718I 497 GGTACC G^GTACC
AvaI 1111 CTCGAG C^[CT]CG[GA]G
AviII 69 TGCGCA TGC^GCA
BalI 1130 TGGCCA TGG^CCA
BanII 1000 GGGCCC G[GA]GC[CT]^C
BbsI 940 GTCTTC GAAGAC(2/6)
BbvCI 699 CCTCAGC CCTCAGC(-5/-2)
BcgI 582 CGATGATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 544 GGATAC GTATCC(6/5)
BcnI 876 CCGGG CC^[GC]GG
BfrI 712 CTTAAG C^TTAAG
BpuAI 940 GTCTTC GAAGAC(2/6)
BsaBI 765 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1287 GAGGAG GAGGAG(10/8)
BsgI 519 CTGCAC GTGCAG(16/14)
BsmI 1338 GAATGC GAATGC(1/-1)
BsoBI 1111 CTCGAG C^[CT]CG[GA]G
BspDI 211 ATCGAT AT^CGAT
BsrDI 201 CATTGC GCAATG(2/0)
BstUI 1294 CGCG CG^CG
ClaI 211 ATCGAT AT^CGAT
DpnII 1348 GATC ^GATC
DraI 306 TTTAAA TTT^AAA
DraIII 391 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 176 CCGTGG C^C[GA][CT]GG
EcoNI 683 CCTATCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 524 CCTTGG C^C[AT][AT]GG
FauI 1358 CCCGC CCCGC(4/6)
FspI 69 TGCGCA TGC^GCA
HapII 876 CCGG C^CGG
HgaI 1292 GACGC GACGC(5/10)
HindIII 709 AAGCTT A^AGCTT
HpaII 876 CCGG C^CGG
KpnI 497 GGTACC GGTAC^C
MamI 765 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1348 GATC ^GATC
MluNI 1130 TGGCCA TGG^CCA
MscI 1130 TGGCCA TGG^CCA
MspI 876 CCGG C^CGG
MvnI 1294 CGCG CG^CG
NciI 876 CCGGG CC^[GC]GG
NdeII 1348 GATC ^GATC
NheI 1179 GCTAGC G^CTAGC
PaeR7I 1111 CTCGAG C^TCGAG
PflMI 157 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 168 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 142 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 796 ATTAAT AT^TAAT
Sau3AI 1348 GATC ^GATC
StyI 524 CCTTGG C^C[AT][AT]GG
Tsp45I 397 GTGAC ^GT[GC]AC
Van91I 157 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 206 CCAGCATCGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1111 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 917 ACATGT A^C[GA][CT]GT
1260 ACATGT
AocI 489 CCTAAGG CC^T[ACGT]AGG
1284 CCTGAGG
AspHI 388 GTGCAC G[AT]GC[AT]^C
1104 GAGCAC
BpmI 1194 CTCCAG CTGGAG(16/14)
270 CTGGAG
BsiHKAI 388 GTGCAC G[AT]GC[AT]^C
1104 GAGCAC
BsmBI 112 GAGACG CGTCTC(1/5)
1290 GAGACG
BspLU11I 917 ACATGT A^CATGT
1260 ACATGT
Bsu36I 489 CCTAAGG CC^T[ACGT]AGG
1284 CCTGAGG
EaeI 1130 TGGCCA [CT]^GGCC[GA]
1239 TGGCCG
Eco81I 489 CCTAAGG CC^T[ACGT]AGG
1284 CCTGAGG
HincII 976 GTCAAC GT[CT]^[GA]AC
1069 GTCAAC
HindII 976 GTCAAC GT[CT]^[GA]AC
1069 GTCAAC
HphI 653 TCACC GGTGA(8/7)
1313 TCACC
SmlI 712 CTTAAG C^T[CT][GA]AG
1111 CTCGAG
TaqI 212 TCGA T^CGA
1112 TCGA
TthHB8I 212 TCGA T^CGA
1112 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BfaI BmyI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeIII
MboII MnlI MseI MslI MspA1I
MvaI MwoI NlaIII NlaIV NspI
PleI PvuII RsaI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI