Restriction map for
pF1KB3810
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 599 TCCGGA T^CCGGA
AciI 431 CCGC CCGC(-3/-1)
AvaI 900 CTCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BbvI 1438 GCAGC GCAGC(8/12)
BcgI 517 GCAGGCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 920 TGATCA T^GATCA
BpmI 496 CTGGAG CTGGAG(16/14)
BseAI 599 TCCGGA T^CCGGA
BsmBI 355 GAGACG CGTCTC(1/5)
BsoBI 900 CTCGGG C^[CT]CG[GA]G
BspEI 599 TCCGGA T^CCGGA
BspMI 763 ACCTGC ACCTGC(4/8)
BsrDI 767 GCAATG GCAATG(2/0)
BsrFI 575 GCCGGT [GA]^CCGG[CT]
BstEII 442 GGTGACC G^GT[ACGT]ACC
BstPI 442 GGTGACC G^GT[ACGT]ACC
Cfr10I 575 GCCGGT [GA]^CCGG[CT]
DraIII 607 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO65I 442 GGTGACC G^GT[ACGT]ACC
FbaI 920 TGATCA T^GATCA
MluNI 2 TGGCCA TGG^CCA
MroI 599 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
PpuMI 1165 GGGTCCC [GA]G^G[AT]CC[CT]
SfaNI 1126 GCATC GCATC(5/9)
SfcI 1406 CTATAG C^T[GA][CT]AG
SmlI 1121 CTCAAG C^T[CT][GA]AG
SphI 426 GCATGC GCATG^C
TaqI 687 TCGA T^CGA
TseI 1438 GCAGC G^C[AT]GC
TthHB8I 687 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 228 GTAC GT^AC
1466 GTAC
Alw44I 616 GTGCAC G^TGCAC
653 GTGCAC
ApaLI 616 GTGCAC G^TGCAC
653 GTGCAC
AvaII 1166 GGTCC G^G[AT]CC
1285 GGTCC
BamHI 1152 GGATCC G^GATCC
1392 GGATCC
BanII 793 GAGCTC G[GA]GC[CT]^C
897 GAGCTC
BfaI 1144 CTAG C^TAG
1171 CTAG
BsaI 1187 GAGACC GGTCTC(1/5)
1418 GGTCTC
BsmFI 52 GTCCC GGGAC(10/14)
1167 GTCCC
BssKI 16 CCTGG ^CC[ACGT]GG
1335 CCAGG
BstNI 16 CCTGG CC^[AT]GG
1335 CCAGG
DraI 406 TTTAAA TTT^AAA
1278 TTTAAA
DraII 178 AGGCCCT [GA]G^G[ACGT]CC[CT]
1165 GGGTCCC
EarI 1104 CTCTTC CTCTTC(1/4)
699 GAAGAG
EcoO109I 178 AGGCCCT [GA]G^G[ACGT]CC[CT]
1165 GGGTCCC
EcoRII 16 CCTGG ^CC[AT]GG
1335 CCAGG
EcoT14I 209 CCAAGG C^C[AT][AT]GG
1317 CCTTGG
Fnu4HI 430 GCCGC GC^[ACGT]GC
1438 GCAGC
HindIII 397 AAGCTT A^AGCTT
1490 AAGCTT
ItaI 430 GCCGC GC^[ACGT]GC
1438 GCAGC
Ksp632I 1104 CTCTTC CTCTTC(1/4)
699 GAAGAG
MaeI 1144 CTAG C^TAG
1171 CTAG
MslI 483 CACGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
609 CATGGTGGTG
MvaI 16 CCTGG CC^[AT]GG
1335 CCAGG
NdeI 691 CATATG CA^TATG
1019 CATATG
NspI 426 GCATGC [GA]CATG^[CT]
946 ACATGC
PleI 684 GACTC GAGTC(4/5)
702 GAGTC
RsaI 228 GTAC GT^AC
1466 GTAC
SacI 793 GAGCTC GAGCT^C
897 GAGCTC
ScrFI 16 CCTGG CC^[ACGT]GG
1335 CCAGG
StuI 122 AGGCCT AGG^CCT
212 AGGCCT
StyI 209 CCAAGG C^C[AT][AT]GG
1317 CCTTGG
TfiI 324 GAATC G^A[AT]TC
1117 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI AlwI AspHI BmyI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII FokI
HaeIII HapII HinfI HpaII HphI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I Tru9I
Tsp45I Tsp509I TspRI XhoII