Restriction map for
pF1KB3856
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BciVI BclI BfrI
BlnI BsiWI BspDI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 3198 CGCG CG^CG
AgeI 827 ACCGGT A^CCGGT
AviII 2753 TGCGCA TGC^GCA
BbrPI 1090 CACGTG CAC^GTG
BbvCI 400 GCTGAGG CCTCAGC(-5/-2)
BcgI 642 CGACGGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 808 CTAG C^TAG
BsaAI 1090 CACGTG [CT]AC^GT[GA]
BsaBI 1212 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2454 CGTCTC CGTCTC(1/5)
Bsp1407I 3368 TGTACA T^GTACA
BsrGI 3368 TGTACA T^GTACA
BstUI 3198 CGCG CG^CG
DrdI 1384 GACCCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 150 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2753 TGCGCA TGC^GCA
MaeI 808 CTAG C^TAG
MamI 1212 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 3389 TTAA T^TAA
MvnI 3198 CGCG CG^CG
NspI 3071 GCATGC [GA]CATG^[CT]
PinAI 827 ACCGGT A^CCGGT
PmaCI 1090 CACGTG CAC^GTG
PmlI 1090 CACGTG CAC^GTG
SacI 1123 GAGCTC GAGCT^C
SapI 2955 GAAGAGC GCTCTTC(1/4)
SmlI 1900 CTCAAG C^T[CT][GA]AG
SphI 3071 GCATGC GCATG^C
SspBI 3368 TGTACA T^GTACA
StuI 2327 AGGCCT AGG^CCT
TaqI 318 TCGA T^CGA
Tru9I 3389 TTAA T^TAA
TthHB8I 318 TCGA T^CGA
XcmI 2828 CCATCCTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccIII 270 TCCGGA T^CCGGA
3473 TCCGGA
Aor51HI 1073 AGCGCT AGC^GCT
2241 AGCGCT
Asp700I 1299 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2532 GAAGATCTTC
BamHI 2789 GGATCC G^GATCC
3395 GGATCC
BglII 2474 AGATCT A^GATCT
2534 AGATCT
BseAI 270 TCCGGA T^CCGGA
3473 TCCGGA
BsmI 873 GAATGC GAATGC(1/-1)
1536 GAATGC
BspEI 270 TCCGGA T^CCGGA
3473 TCCGGA
BspHI 815 TCATGA T^CATGA
3209 TCATGA
EagI 800 CGGCCG C^GGCCG
1716 CGGCCG
EclXI 800 CGGCCG C^GGCCG
1716 CGGCCG
Eco47III 1073 AGCGCT AGC^GCT
2241 AGCGCT
Eco52I 800 CGGCCG C^GGCCG
1716 CGGCCG
MroI 270 TCCGGA T^CCGGA
3473 TCCGGA
NaeI 1922 GCCGGC GCC^GGC
2725 GCCGGC
NgoMIV 1922 GCCGGC G^CCGGC
2725 GCCGGC
RcaI 815 TCATGA T^CATGA
3209 TCATGA
SexAI 1196 ACCAGGT A^CC[AT]GGT
2366 ACCTGGT
XmnI 1299 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2532 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TfiI TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmaCI XmaI