Restriction map for
pF1KB3889
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# NO CUT ENZYMES ( 77 enzymes) #
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AccI AclI AcsI AflII AflIII
ApoI AscI AseI AsnI AviII
AvrII BamHI BbeI BciVI BclI
BfrI BlnI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KasI
MfeI MluI MseI MunI NarI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SfoI SfuI
SmaI SnaBI SpeI SphI SspI
SwaI TfiI Tru9I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1095 GACGTC GACGT^C
Acc65I 1994 GGTACC G^GTACC
AcyI 1095 GACGTC G[GA]^CG[CT]C
AgeI 551 ACCGGT A^CCGGT
AhdI 246 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 460 GTGCAC G^TGCAC
AocI 2077 CCTGAGG CC^T[ACGT]AGG
ApaLI 460 GTGCAC G^TGCAC
Asp700I 981 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1994 GGTACC G^GTACC
AspEI 246 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1816 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2157 CACGTG CAC^GTG
BbsI 1546 GTCTTC GAAGAC(2/6)
BcgI 689 CGAGGACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 650 AGATCT A^GATCT
BpuAI 1546 GTCTTC GAAGAC(2/6)
BsaAI 2157 CACGTG [CT]AC^GT[GA]
BsaBI 651 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1095 GACGTC G[GA]^CG[CT]C
BsaI 7 GAGACC GGTCTC(1/5)
Bsp1407I 845 TGTACA T^GTACA
BsrBI 2293 GAGCGG CCGCTC(-3/-3)
BsrDI 260 CATTGC GCAATG(2/0)
BsrGI 845 TGTACA T^GTACA
BssHII 2414 GCGCGC G^CGCGC
BstAPI 1274 GCACCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2077 CCTGAGG CC^T[ACGT]AGG
DrdI 1876 GACTCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 246 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2077 CCTGAGG CC^T[ACGT]AGG
EcoRV 1129 GATATC GAT^ATC
Hin1I 1095 GACGTC G[GA]^CG[CT]C
HincII 139 GTCAAC GT[CT]^[GA]AC
HindII 139 GTCAAC GT[CT]^[GA]AC
KpnI 1994 GGTACC GGTAC^C
KspI 11 CCGCGG CCGC^GG
MamI 651 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 369 GCTAGC G^CTAGC
PflFI 1816 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 551 ACCGGT A^CCGGT
PmaCI 2157 CACGTG CAC^GTG
PmlI 2157 CACGTG CAC^GTG
SacII 11 CCGCGG CCGC^GG
SapI 501 GCTCTTC GCTCTTC(1/4)
SbfI 1404 CCTGCAGG CCTGCA^GG
SexAI 1598 ACCAGGT A^CC[AT]GGT
Sse8387I 1404 CCTGCAGG CCTGCA^GG
SspBI 845 TGTACA T^GTACA
TaqI 350 TCGA T^CGA
Tsp509I 60 AATT ^AATT
Tth111I 1816 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 350 TCGA T^CGA
XmnI 981 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccIII 1154 TCCGGA T^CCGGA
2066 TCCGGA
Aor51HI 1424 AGCGCT AGC^GCT
2265 AGCGCT
AspHI 460 GTGCAC G[AT]GC[AT]^C
2209 GTGCTC
BbvCI 219 CCTCAGC CCTCAGC(-5/-2)
1616 CCTCAGC
BlpI 630 GCTCAGC GC^T[ACGT]AGC
2310 GCTCAGC
Bpu1102I 630 GCTCAGC GC^T[ACGT]AGC
2310 GCTCAGC
BseAI 1154 TCCGGA T^CCGGA
2066 TCCGGA
BsiHKAI 460 GTGCAC G[AT]GC[AT]^C
2209 GTGCTC
BsmAI 7 GAGAC GTCTC(1/5)
1664 GTCTC
BspEI 1154 TCCGGA T^CCGGA
2066 TCCGGA
BstXI 1557 CCACCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2018 CCATCTCCCTGG
BstYI 650 AGATCT [GA]^GATC[CT]
1109 AGATCC
CelII 630 GCTCAGC GC^T[ACGT]AGC
2310 GCTCAGC
Eco47III 1424 AGCGCT AGC^GCT
2265 AGCGCT
MflI 650 AGATCT [GA]^GATC[CT]
1109 AGATCC
MroI 1154 TCCGGA T^CCGGA
2066 TCCGGA
MslI 528 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1558 CACCGGCGTG
NcoI 341 CCATGG C^CATGG
1592 CCATGG
SgrAI 909 CGCCGGCG C[GA]^CCGG[CT]G
1558 CACCGGCG
StuI 774 AGGCCT AGG^CCT
2081 AGGCCT
XcmI 38 CCAACTATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1854 CCACGCCCTCACTGG
XhoII 650 AGATCT [GA]^GATC[CT]
1109 AGATCC
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiEI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmlI
StyI TseI Tsp45I TspRI Van91I