Restriction map for
pF1KB3902
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MamI MflI
MluI MroI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1823 GACGTC GACGT^C
AcsI 1381 AAATTT [GA]^AATT[CT]
AcyI 1823 GACGTC G[GA]^CG[CT]C
AhdI 444 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 703 GGATC GGATC(4/5)
AocI 1678 CCTGAGG CC^T[ACGT]AGG
ApaI 1489 GGGCCC GGGCC^C
ApoI 1381 AAATTT [GA]^AATT[CT]
AspEI 444 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1176 TGGCCA TGG^CCA
BciVI 1294 GTATCC GTATCC(6/5)
BclI 1355 TGATCA T^GATCA
BfaI 1645 CTAG C^TAG
BsaHI 1823 GACGTC G[GA]^CG[CT]C
BsaWI 307 ACCGGA [AT]^CCGG[AT]
BsiEI 139 CGGCCG CG[GA][CT]^CG
BsiWI 660 CGTACG C^GTACG
BsmAI 7 GTCTC GTCTC(1/5)
BsmI 350 GCATTC GAATGC(1/-1)
BspHI 1352 TCATGA T^CATGA
BssSI 426 CTCGTG CACGAG(-5/-1)
Bsu36I 1678 CCTGAGG CC^T[ACGT]AGG
DraII 1395 GGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 139 CGGCCG C^GGCCG
Eam1105I 444 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 139 CGGCCG C^GGCCG
Eco52I 139 CGGCCG C^GGCCG
Eco81I 1678 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1395 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 1026 GCGGG CCCGC(4/6)
FbaI 1355 TGATCA T^GATCA
Hin1I 1823 GACGTC G[GA]^CG[CT]C
MaeI 1645 CTAG C^TAG
MfeI 129 CAATTG C^AATTG
MluNI 1176 TGGCCA TGG^CCA
MscI 1176 TGGCCA TGG^CCA
MunI 129 CAATTG C^AATTG
NaeI 134 GCCGGC GCC^GGC
NcoI 1779 CCATGG C^CATGG
NgoMIV 134 GCCGGC G^CCGGC
PpuMI 1395 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 492 CAGCTG CAG^CTG
RcaI 1352 TCATGA T^CATGA
SacI 298 GAGCTC GAGCT^C
SalI 1370 GTCGAC G^TCGAC
ScaI 1544 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 901 GTCTAC GT^[AC][GT]AC
1370 GTCGAC
AfaI 661 GTAC GT^AC
1545 GTAC
AflIII 915 ACGTGT A^C[GA][CT]GT
1580 ACACGT
AlwNI 442 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
1018 CAGCCTCTG
Asp700I 153 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAACTTTTTC
BbrPI 914 CACGTG CAC^GTG
1581 CACGTG
BcnI 1487 CCGGG CC^[GC]GG
1756 CCCGG
BpmI 928 CTGGAG CTGGAG(16/14)
949 CTGGAG
BsaAI 914 CACGTG [CT]AC^GT[GA]
1581 CACGTG
BseRI 931 GAGGAG GAGGAG(10/8)
1420 GAGGAG
BsrFI 134 GCCGGC [GA]^CCGG[CT]
410 ACCGGC
BstAPI 999 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1017 GCAGCCTCTGC
CfoI 264 GCGC GCG^C
945 GCGC
Cfr10I 134 GCCGGC [GA]^CCGG[CT]
410 ACCGGC
EaeI 139 CGGCCG [CT]^GGCC[GA]
1176 TGGCCA
EcoT14I 1708 CCAAGG C^C[AT][AT]GG
1779 CCATGG
HhaI 264 GCGC GCG^C
945 GCGC
HinP1I 264 GCGC G^CGC
945 GCGC
HincII 649 GTCAAC GT[CT]^[GA]AC
1370 GTCGAC
HindII 649 GTCAAC GT[CT]^[GA]AC
1370 GTCGAC
MseI 1618 TTAA T^TAA
1775 TTAA
MspA1I 492 CAGCTG C[AC]G^C[GT]G
504 CCGCTG
NciI 1487 CCGGG CC^[GC]GG
1756 CCCGG
PmaCI 914 CACGTG CAC^GTG
1581 CACGTG
PmlI 914 CACGTG CAC^GTG
1581 CACGTG
RsaI 661 GTAC GT^AC
1545 GTAC
SapI 435 GCTCTTC GCTCTTC(1/4)
1089 GCTCTTC
SmlI 228 CTCAAG C^T[CT][GA]AG
1186 CTCAAG
StyI 1708 CCAAGG C^C[AT][AT]GG
1779 CCATGG
TaqI 962 TCGA T^CGA
1371 TCGA
TfiI 181 GATTC G^A[AT]TC
1740 GAATC
Tru9I 1618 TTAA T^TAA
1775 TTAA
TthHB8I 962 TCGA T^CGA
1371 TCGA
XmnI 153 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbsI BbvI BmyI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DrdI DsaI EarI EcoRII Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
Tsp509I TspRI XcmI