Restriction map for
pF1KB4020
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BfrI BglII BsaAI BsaBI
BsaHI BseAI BsgI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MluI
MroI MslI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 130 GTCTAC GT^[AC][GT]AC
AgeI 1442 ACCGGT A^CCGGT
ApaI 746 GGGCCC GGGCC^C
AscI 20 GGCGCGCC GG^CGCGCC
AspI 6 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 525 CCTAGG C^CTAGG
BbsI 792 GAAGAC GAAGAC(2/6)
BcgI 887 GCATCCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 80 CCCGG CC^[GC]GG
BlnI 525 CCTAGG C^CTAGG
BpuAI 792 GAAGAC GAAGAC(2/6)
BsaWI 1442 ACCGGT [AT]^CCGG[AT]
BsiEI 138 CGACCG CG[GA][CT]^CG
BsiWI 1579 CGTACG C^GTACG
BspHI 663 TCATGA T^CATGA
BssSI 865 CACGAG CACGAG(-5/-1)
BstYI 1115 AGATCC [GA]^GATC[CT]
DraIII 1226 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1915 GACAATCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 525 CCTAGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1241 GCGTC GACGC(5/10)
MfeI 1395 CAATTG C^AATTG
MflI 1115 AGATCC [GA]^GATC[CT]
MseI 338 TTAA T^TAA
MunI 1395 CAATTG C^AATTG
NciI 80 CCCGG CC^[GC]GG
NcoI 1388 CCATGG C^CATGG
PflFI 6 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1383 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1442 ACCGGT A^CCGGT
RcaI 663 TCATGA T^CATGA
SapI 1841 GCTCTTC GCTCTTC(1/4)
SbfI 102 CCTGCAGG CCTGCA^GG
SmlI 514 CTTGAG C^T[CT][GA]AG
Sse8387I 102 CCTGCAGG CCTGCA^GG
Tru9I 338 TTAA T^TAA
Tsp509I 1396 AATT ^AATT
Tth111I 6 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1383 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1115 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1116 GATCC GGATC(4/5)
1426 GATCC
AvaI 253 CTCGGG C^[CT]CG[GA]G
1716 CTCGGG
AviII 1361 TGCGCA TGC^GCA
1619 TGCGCA
BalI 506 TGGCCA TGG^CCA
1613 TGGCCA
BfaI 526 CTAG C^TAG
1307 CTAG
BlpI 315 GCTGAGC GC^T[ACGT]AGC
915 GCTCAGC
Bpu1102I 315 GCTGAGC GC^T[ACGT]AGC
915 GCTCAGC
BsoBI 253 CTCGGG C^[CT]CG[GA]G
1716 CTCGGG
BstAPI 486 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
735 GCAGGAACTGC
BstEII 373 GGTGACC G^GT[ACGT]ACC
1923 GGTCACC
BstPI 373 GGTGACC G^GT[ACGT]ACC
1923 GGTCACC
CelII 315 GCTGAGC GC^T[ACGT]AGC
915 GCTCAGC
EarI 1842 CTCTTC CTCTTC(1/4)
199 GAAGAG
EcoO65I 373 GGTGACC G^GT[ACGT]ACC
1923 GGTCACC
FspI 1361 TGCGCA TGC^GCA
1619 TGCGCA
HaeII 656 AGCGCC [GA]GCGC^[CT]
1409 AGCGCC
HindIII 511 AAGCTT A^AGCTT
955 AAGCTT
Ksp632I 1842 CTCTTC CTCTTC(1/4)
199 GAAGAG
MaeI 526 CTAG C^TAG
1307 CTAG
MluNI 506 TGGCCA TGG^CCA
1613 TGGCCA
MscI 506 TGGCCA TGG^CCA
1613 TGGCCA
NaeI 716 GCCGGC GCC^GGC
1001 GCCGGC
NgoMIV 716 GCCGGC G^CCGGC
1001 GCCGGC
SacI 109 GAGCTC GAGCT^C
250 GAGCTC
XcmI 1221 CCAGACACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1616 CCATGCGCAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanII BbvI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I