Restriction map for
pF1KB4092
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 805 TCCGGA T^CCGGA
AcsI 1083 GAATTC [GA]^AATT[CT]
AgeI 239 ACCGGT A^CCGGT
AlwNI 792 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 13 CCTGAGG CC^T[ACGT]AGG
ApoI 1083 GAATTC [GA]^AATT[CT]
Asp700I 376 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 852 GAGCAC G[AT]GC[AT]^C
AvaI 637 CCCGGG C^[CT]CG[GA]G
BanI 700 GGTGCC G^G[CT][GA]CC
BbvCI 789 CCTCAGC CCTCAGC(-5/-2)
BpmI 1174 CTGGAG CTGGAG(16/14)
BsaI 785 GAGACC GGTCTC(1/5)
BseAI 805 TCCGGA T^CCGGA
BsiEI 899 CGACCG CG[GA][CT]^CG
BsiHKAI 852 GAGCAC G[AT]GC[AT]^C
BsoBI 637 CCCGGG C^[CT]CG[GA]G
BspEI 805 TCCGGA T^CCGGA
BspMI 121 GCAGGT ACCTGC(4/8)
BsrI 1182 CCAGT ACTGG(1/-1)
BstYI 585 AGATCC [GA]^GATC[CT]
Bsu36I 13 CCTGAGG CC^T[ACGT]AGG
CfoI 320 GCGC GCG^C
DsaI 45 CCGTGG C^C[GA][CT]GG
EaeI 616 CGGCCA [CT]^GGCC[GA]
Eco81I 13 CCTGAGG CC^T[ACGT]AGG
EcoNI 13 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1083 GAATTC G^AATTC
HaeII 319 AGCGCC [GA]GCGC^[CT]
HhaI 320 GCGC GCG^C
HinP1I 320 GCGC G^CGC
MaeII 77 ACGT A^CGT
MflI 585 AGATCC [GA]^GATC[CT]
MroI 805 TCCGGA T^CCGGA
MseI 551 TTAA T^TAA
MspA1I 613 CAGCGG C[AC]G^C[GT]G
NaeI 1207 GCCGGC GCC^GGC
NgoMIV 1207 GCCGGC G^CCGGC
PinAI 239 ACCGGT A^CCGGT
SmaI 637 CCCGGG CCC^GGG
TaqI 345 TCGA T^CGA
TfiI 105 GATTC G^A[AT]TC
Tru9I 551 TTAA T^TAA
Tsp45I 979 GTGAC ^GT[GC]AC
TthHB8I 345 TCGA T^CGA
XhoII 585 AGATCC [GA]^GATC[CT]
XmaCI 637 CCCGGG C^CCGGG
XmaI 637 CCCGGG C^CCGGG
XmnI 376 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 9 GGGCCC GGGCC^C
622 GGGCCC
BanII 9 GGGCCC G[GA]GC[CT]^C
622 GGGCCC
BcnI 637 CCCGG CC^[GC]GG
638 CCGGG
BfaI 143 CTAG C^TAG
505 CTAG
BsaWI 239 ACCGGT [AT]^CCGG[AT]
805 TCCGGA
BseRI 929 CTCCTC GAGGAG(10/8)
730 GAGGAG
BsmAI 785 GAGAC GTCTC(1/5)
800 GTCTC
BspHI 108 TCATGA T^CATGA
1011 TCATGA
BsrDI 425 CATTGC GCAATG(2/0)
1103 GCAATG
BstF5I 361 GGATG GGATG(2/0)
689 GGATG
EarI 1075 CTCTTC CTCTTC(1/4)
974 GAAGAG
FokI 361 GGATG GGATG(9/13)
689 GGATG
Ksp632I 1075 CTCTTC CTCTTC(1/4)
974 GAAGAG
MaeI 143 CTAG C^TAG
505 CTAG
MaeIII 979 GTGAC ^GT[ACGT]AC
1199 GTTAC
NciI 637 CCCGG CC^[GC]GG
638 CCGGG
PleI 350 GACTC GAGTC(4/5)
947 GAGTC
RcaI 108 TCATGA T^CATGA
1011 TCATGA
SfcI 705 CTACAG C^T[GA][CT]AG
1212 CTACAG
StuI 678 AGGCCT AGG^CCT
694 AGGCCT
XcmI 951 CCATATTGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1182 CCAGTCAGTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BsrFI BssKI
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MboI MboII MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp509I TspRI