Restriction map for
pF1KB4122
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
Aor51HI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MslI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 28 CGCG CG^CG
AfaI 1304 GTAC GT^AC
AocI 616 CCTAAGG CC^T[ACGT]AGG
ApaI 79 GGGCCC GGGCC^C
AseI 1399 ATTAAT AT^TAAT
AsnI 1399 ATTAAT AT^TAAT
AvaI 527 CTCGGG C^[CT]CG[GA]G
BanI 519 GGCACC G^G[CT][GA]CC
BanII 79 GGGCCC G[GA]GC[CT]^C
BciVI 1109 GTATCC GTATCC(6/5)
BcnI 33 CCGGG CC^[GC]GG
BpmI 1203 CTCCAG CTGGAG(16/14)
BsaAI 632 TACGTG [CT]AC^GT[GA]
BsaI 23 GAGACC GGTCTC(1/5)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmI 755 GAATGC GAATGC(1/-1)
BsoBI 527 CTCGGG C^[CT]CG[GA]G
Bst1107I 1658 GTATAC GTA^TAC
BstAPI 1780 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 28 CGCG CG^CG
BstYI 421 GGATCT [GA]^GATC[CT]
BstZ17I 1658 GTATAC GTA^TAC
Bsu36I 616 CCTAAGG CC^T[ACGT]AGG
CfoI 201 GCGC GCG^C
EagI 30 CGGCCG C^GGCCG
EarI 1000 GAAGAG CTCTTC(1/4)
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
Eco81I 616 CCTAAGG CC^T[ACGT]AGG
EcoRI 1156 GAATTC G^AATTC
EcoT22I 1778 ATGCAT ATGCA^T
FauI 1495 GCGGG CCCGC(4/6)
HapII 33 CCGG C^CGG
HhaI 201 GCGC GCG^C
HinP1I 201 GCGC G^CGC
HincII 1127 GTCAAC GT[CT]^[GA]AC
HindII 1127 GTCAAC GT[CT]^[GA]AC
HpaII 33 CCGG C^CGG
HphI 1117 GGTGA GGTGA(8/7)
Ksp632I 1000 GAAGAG CTCTTC(1/4)
KspI 27 CCGCGG CCGC^GG
MflI 421 GGATCT [GA]^GATC[CT]
MspI 33 CCGG C^CGG
MvnI 28 CGCG CG^CG
NciI 33 CCGGG CC^[GC]GG
NdeI 1775 CATATG CA^TATG
NsiI 1778 ATGCAT ATGCA^T
NspI 577 GCATGT [GA]CATG^[CT]
PleI 1125 GAGTC GAGTC(4/5)
PshAI 1120 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1399 ATTAAT AT^TAAT
PstI 1793 CTGCAG CTGCA^G
PvuII 1796 CAGCTG CAG^CTG
RsaI 1304 GTAC GT^AC
SacII 27 CCGCGG CCGC^GG
SspI 566 AATATT AAT^ATT
TfiI 138 GAATC G^A[AT]TC
XcmI 1031 CCATTGTTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 421 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 591 GTCTAC GT^[AC][GT]AC
1658 GTATAC
Alw44I 50 GTGCAC G^TGCAC
908 GTGCAC
AlwI 421 GGATC GGATC(4/5)
798 GGATC
ApaLI 50 GTGCAC G^TGCAC
908 GTGCAC
AvaII 36 GGACC G^G[AT]CC
622 GGACC
BalI 373 TGGCCA TGG^CCA
844 TGGCCA
BbsI 677 GAAGAC GAAGAC(2/6)
1426 GAAGAC
BpuAI 677 GAAGAC GAAGAC(2/6)
1426 GAAGAC
BsgI 1799 CTGCAC GTGCAG(16/14)
547 GTGCAG
BsmAI 23 GAGAC GTCTC(1/5)
1526 GAGAC
BspHI 960 TCATGA T^CATGA
1007 TCATGA
DraII 78 AGGGCCC [GA]G^G[ACGT]CC[CT]
79 GGGCCCT
EcoO109I 78 AGGGCCC [GA]G^G[ACGT]CC[CT]
79 GGGCCCT
EcoT14I 1103 CCATGG C^C[AT][AT]GG
1113 CCATGG
HinfI 138 GAATC G^A[ACGT]TC
1125 GAGTC
MaeII 633 ACGT A^CGT
965 ACGT
MluNI 373 TGGCCA TGG^CCA
844 TGGCCA
MscI 373 TGGCCA TGG^CCA
844 TGGCCA
MspA1I 27 CCGCGG C[AC]G^C[GT]G
1796 CAGCTG
NcoI 1103 CCATGG C^CATGG
1113 CCATGG
RcaI 960 TCATGA T^CATGA
1007 TCATGA
SfcI 1190 CTACAG C^T[GA][CT]AG
1793 CTGCAG
StyI 1103 CCATGG C^C[AT][AT]GG
1113 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
BbvI BfaI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII Fnu4HI FokI HaeIII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I