################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 394 AACGTT AA^CGTT AflIII 809 ACATGT A^C[GA][CT]GT AlwNI 860 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1282 CCTCAGG CC^T[ACGT]AGG BbvI 170 GCAGC GCAGC(8/12) BfaI 1109 CTAG C^TAG BpmI 319 CTGGAG CTGGAG(16/14) BsaJI 566 CCTGGG C^C[ACGT][ACGT]GG BsaWI 133 ACCGGA [AT]^CCGG[AT] BsmFI 162 GGGAC GGGAC(10/14) BsmI 1102 GCATTC GAATGC(1/-1) BspLU11I 809 ACATGT A^CATGT BspMI 438 ACCTGC ACCTGC(4/8) BssKI 566 CCTGG ^CC[ACGT]GG BstBI 719 TTCGAA TT^CGAA BstNI 566 CCTGG CC^[AT]GG Bsu36I 1282 CCTCAGG CC^T[ACGT]AGG Cac8I 885 GCGAGC GC[ACGT]^[ACGT]GC Cfr13I 1018 GGCCC G^G[ACGT]CC DdeI 1283 CTCAG C^T[ACGT]AG DraI 993 TTTAAA TTT^AAA Eco81I 1282 CCTCAGG CC^T[ACGT]AGG EcoRI 449 GAATTC G^AATTC EcoRII 566 CCTGG ^CC[AT]GG EcoRV 483 GATATC GAT^ATC EcoT22I 1100 ATGCAT ATGCA^T Fnu4HI 170 GCAGC GC^[ACGT]GC HaeIII 1018 GGCC GG^CC HindIII 114 AAGCTT A^AGCTT ItaI 170 GCAGC GC^[ACGT]GC MaeI 1109 CTAG C^TAG MaeII 395 ACGT A^CGT MvaI 566 CCTGG CC^[AT]GG NsiI 1100 ATGCAT ATGCA^T NspV 719 TTCGAA TT^CGAA PleI 929 GAGTC GAGTC(4/5) Psp1406I 394 AACGTT AA^CGTT Sau96I 1018 GGCCC G^G[ACGT]CC ScrFI 566 CCTGG CC^[ACGT]GG SfuI 719 TTCGAA TT^CGAA TaqI 720 TCGA T^CGA TseI 170 GCAGC G^C[AT]GC TthHB8I 720 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BbsI 548 GTCTTC GAAGAC(2/6) 931 GTCTTC BpuAI 548 GTCTTC GAAGAC(2/6) 931 GTCTTC BsmAI 126 GTCTC GTCTC(1/5) 379 GTCTC BsrI 318 ACTGG ACTGG(1/-1) 618 ACTGG BstF5I 280 GGATG GGATG(2/0) 938 GGATG BstYI 647 AGATCC [GA]^GATC[CT] 1218 AGATCC FokI 280 GGATG GGATG(9/13) 938 GGATG HapII 134 CCGG C^CGG 882 CCGG HpaII 134 CCGG C^CGG 882 CCGG MflI 647 AGATCC [GA]^GATC[CT] 1218 AGATCC MspI 134 CCGG C^CGG 882 CCGG NspI 29 ACATGC [GA]CATG^[CT] 809 ACATGT SspI 654 AATATT AAT^ATT 715 AATATT XhoII 647 AGATCC [GA]^GATC[CT] 1218 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI DpnII HinfI HphI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII RsaI Sau3AI SfcI TfiI Tru9I Tsp45I Tsp509I TspRI