Restriction map for pF1KB4126
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AclI          394  AACGTT               AA^CGTT
AflIII        809  ACATGT               A^C[GA][CT]GT
AlwNI         860  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1282  CCTCAGG              CC^T[ACGT]AGG
BbvI          170  GCAGC                GCAGC(8/12)
BfaI         1109  CTAG                 C^TAG
BpmI          319  CTGGAG               CTGGAG(16/14)
BsaJI         566  CCTGGG               C^C[ACGT][ACGT]GG
BsaWI         133  ACCGGA               [AT]^CCGG[AT]
BsmFI         162  GGGAC                GGGAC(10/14)
BsmI         1102  GCATTC               GAATGC(1/-1)
BspLU11I      809  ACATGT               A^CATGT
BspMI         438  ACCTGC               ACCTGC(4/8)
BssKI         566  CCTGG                ^CC[ACGT]GG
BstBI         719  TTCGAA               TT^CGAA
BstNI         566  CCTGG                CC^[AT]GG
Bsu36I       1282  CCTCAGG              CC^T[ACGT]AGG
Cac8I         885  GCGAGC               GC[ACGT]^[ACGT]GC
Cfr13I       1018  GGCCC                G^G[ACGT]CC
DdeI         1283  CTCAG                C^T[ACGT]AG
DraI          993  TTTAAA               TTT^AAA
Eco81I       1282  CCTCAGG              CC^T[ACGT]AGG
EcoRI         449  GAATTC               G^AATTC
EcoRII        566  CCTGG                ^CC[AT]GG
EcoRV         483  GATATC               GAT^ATC
EcoT22I      1100  ATGCAT               ATGCA^T
Fnu4HI        170  GCAGC                GC^[ACGT]GC
HaeIII       1018  GGCC                 GG^CC
HindIII       114  AAGCTT               A^AGCTT
ItaI          170  GCAGC                GC^[ACGT]GC
MaeI         1109  CTAG                 C^TAG
MaeII         395  ACGT                 A^CGT
MvaI          566  CCTGG                CC^[AT]GG
NsiI         1100  ATGCAT               ATGCA^T
NspV          719  TTCGAA               TT^CGAA
PleI          929  GAGTC                GAGTC(4/5)
Psp1406I      394  AACGTT               AA^CGTT
Sau96I       1018  GGCCC                G^G[ACGT]CC
ScrFI         566  CCTGG                CC^[ACGT]GG
SfuI          719  TTCGAA               TT^CGAA
TaqI          720  TCGA                 T^CGA
TseI          170  GCAGC                G^C[AT]GC
TthHB8I       720  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BbsI          548  GTCTTC               GAAGAC(2/6)
              931  GTCTTC              
BpuAI         548  GTCTTC               GAAGAC(2/6)
              931  GTCTTC              
BsmAI         126  GTCTC                GTCTC(1/5)
              379  GTCTC               
BsrI          318  ACTGG                ACTGG(1/-1)
              618  ACTGG               
BstF5I        280  GGATG                GGATG(2/0)
              938  GGATG               
BstYI         647  AGATCC               [GA]^GATC[CT]
             1218  AGATCC              
FokI          280  GGATG                GGATG(9/13)
              938  GGATG               
HapII         134  CCGG                 C^CGG
              882  CCGG                
HpaII         134  CCGG                 C^CGG
              882  CCGG                
MflI          647  AGATCC               [GA]^GATC[CT]
             1218  AGATCC              
MspI          134  CCGG                 C^CGG
              882  CCGG                
NspI           29  ACATGC               [GA]CATG^[CT]
              809  ACATGT              
SspI          654  AATATT               AAT^ATT
              715  AATATT              
XhoII         647  AGATCC               [GA]^GATC[CT]
             1218  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
DpnII       HinfI       HphI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        NdeII       
NlaIII      RsaI        Sau3AI      SfcI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       
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