##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT14I     FbaI        Fnu4HI      FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HpaI        HpaII       ItaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        TseI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AciI         1199  GCGG                 CCGC(-3/-1)
Aor51HI        43  AGCGCT               AGC^GCT
BbsI          960  GAAGAC               GAAGAC(2/6)
BpmI          674  CTGGAG               CTGGAG(16/14)
BpuAI         960  GAAGAC               GAAGAC(2/6)
BsmAI         579  GAGAC                GTCTC(1/5)
BsmFI          84  GGGAC                GGGAC(10/14)
BspMI         659  ACCTGC               ACCTGC(4/8)
BstF5I       1291  CATCC                GGATG(2/0)
CfoI           44  GCGC                 GCG^C
EarI         1244  CTCTTC               CTCTTC(1/4)
Eco47III       43  AGCGCT               AGC^GCT
EcoT22I      1029  ATGCAT               ATGCA^T
FauI         1199  GCGGG                CCCGC(4/6)
FokI         1291  CATCC                GGATG(9/13)
HaeII          43  AGCGCT               [GA]GCGC^[CT]
HhaI           44  GCGC                 GCG^C
HinP1I         44  GCGC                 G^CGC
Ksp632I      1244  CTCTTC               CTCTTC(1/4)
MwoI          555  GCGATCACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         765  GGAACC               GG[ACGT]^[ACGT]CC
NsiI         1029  ATGCAT               ATGCA^T
NspI          131  ACATGC               [GA]CATG^[CT]
TfiI          918  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BfaI         1295  CTAG                 C^TAG
             1300  CTAG                
BseRI        1279  CTCCTC               GAGGAG(10/8)
               91  GAGGAG              
BsmI          286  GAATGC               GAATGC(1/-1)
             1027  GAATGC              
BspHI         864  TCATGA               T^CATGA
             1023  TCATGA              
DpnII          32  GATC                 ^GATC
              557  GATC                
DraI           10  TTTAAA               TTT^AAA
              319  TTTAAA              
HindIII       813  AAGCTT               A^AGCTT
             1149  AAGCTT              
MaeI         1295  CTAG                 C^TAG
             1300  CTAG                
MboI           32  GATC                 ^GATC
              557  GATC                
NdeII          32  GATC                 ^GATC
              557  GATC                
PleI          665  GACTC                GAGTC(4/5)
             1242  GACTC               
RcaI          864  TCATGA               T^CATGA
             1023  TCATGA              
Sau3AI         32  GATC                 ^GATC
              557  GATC                
SfaNI         720  GCATC                GCATC(5/9)
             1303  GCATC               
SmlI          164  CTCAAG               C^T[CT][GA]AG
              810  CTCAAG              
Tsp45I        242  GTCAC                ^GT[GC]AC
              344  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     BsrDI       
BsrI        DdeI        EcoRI       HaeIII      HinfI       
HphI        MaeIII      MboII       MnlI        MseI        
MslI        NlaIII      StuI        Tru9I       Tsp509I     
TspRI       XmnI