Restriction map for
pF1KB4150
##################################
# NO CUT ENZYMES ( 194 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DdeI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 223 CGCG CG^CG
AfaI 210 GTAC GT^AC
AflIII 185 ACATGT A^C[GA][CT]GT
BamHI 270 GGATCC G^GATCC
BanII 140 GAGCCC G[GA]GC[CT]^C
BmyI 140 GAGCCC G[AGT]GC[ACT]^C
BpmI 95 CTCCAG CTGGAG(16/14)
BsiYI 333 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 333 CCAGCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 130 GAGAC GTCTC(1/5)
Bsp1286I 140 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 185 ACATGT A^CATGT
BssKI 292 CCAGG ^CC[ACGT]GG
BstF5I 266 GGATG GGATG(2/0)
BstNI 292 CCAGG CC^[AT]GG
BstUI 223 CGCG CG^CG
BstXI 319 CCAGAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 270 GGATCC [GA]^GATC[CT]
Cac8I 332 GCCAGC GC[ACGT]^[ACGT]GC
DpnII 271 GATC ^GATC
EarI 33 CTCTTC CTCTTC(1/4)
EcoRII 292 CCAGG ^CC[AT]GG
FokI 266 GGATG GGATG(9/13)
HincII 61 GTTGAC GT[CT]^[GA]AC
HindII 61 GTTGAC GT[CT]^[GA]AC
HinfI 46 GATTC G^A[ACGT]TC
Ksp632I 33 CTCTTC CTCTTC(1/4)
MaeII 212 ACGT A^CGT
MboI 271 GATC ^GATC
MflI 270 GGATCC [GA]^GATC[CT]
MspA1I 334 CAGCTG C[AC]G^C[GT]G
MvaI 292 CCAGG CC^[AT]GG
MvnI 223 CGCG CG^CG
NdeII 271 GATC ^GATC
NlaIII 186 CATG CATG^
NruI 222 TCGCGA TCG^CGA
NspI 185 ACATGT [GA]CATG^[CT]
PvuII 334 CAGCTG CAG^CTG
RsaI 210 GTAC GT^AC
SapI 32 GCTCTTC GCTCTTC(1/4)
Sau3AI 271 GATC ^GATC
ScrFI 292 CCAGG CC^[ACGT]GG
SfaNI 364 GATGC GCATC(5/9)
SspI 260 AATATT AAT^ATT
TaqI 136 TCGA T^CGA
TfiI 46 GATTC G^A[AT]TC
Tsp509I 246 AATT ^AATT
TthHB8I 136 TCGA T^CGA
XhoII 270 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
AluI 31 AGCT AG^CT
335 AGCT
AlwI 271 GATCC GGATC(4/5)
270 GGATC
DraI 229 TTTAAA TTT^AAA
312 TTTAAA
HphI 127 GGTGA GGTGA(8/7)
280 GGTGA
MwoI 32 GCTCTTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
164 GCAGCAGCAGC
NlaIV 270 GGATCC GG[ACGT]^[ACGT]CC
329 GGAGCC
TspRI 42 CAGTG CA[GC]TG[ACGT][ACGT]^
54 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
BbvI Fnu4HI ItaI MboII MnlI
MseI Tru9I TseI