Restriction map for pF1KB4218
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AccIII      AclI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
BbvCI       BfrI        BglI        BglII       BsaBI       
BsaWI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstZ17I     ClaI        
CpoI        DraI        DraIII      Eco47III    EcoNI       
EcoRV       FseI        HindIII     HpaI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SalI        SbfI        ScaI        SfiI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AatII        1327  GACGTC               GACGT^C
Acc65I        433  GGTACC               G^GTACC
AccI         2062  GTCTAC               GT^[AC][GT]AC
AhdI         1603  GACCGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          77  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          880  ATTAAT               AT^TAAT
AsnI          880  ATTAAT               AT^TAAT
Asp718I       433  GGTACC               G^GTACC
AspEI        1603  GACCGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2229  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1871  TGCGCA               TGC^GCA
AvrII         153  CCTAGG               C^CTAGG
BalI         1530  TGGCCA               TGG^CCA
BamHI         652  GGATCC               G^GATCC
BbeI         1594  GGCGCC               GGCGC^C
BbsI          177  GTCTTC               GAAGAC(2/6)
BcgI         2077  GCATTGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1106  GTATCC               GTATCC(6/5)
BclI          381  TGATCA               T^GATCA
BlnI          153  CCTAGG               C^CTAGG
BlpI         1887  GCTGAGC              GC^T[ACGT]AGC
BpmI         1124  CTGGAG               CTGGAG(16/14)
Bpu1102I     1887  GCTGAGC              GC^T[ACGT]AGC
BpuAI         177  GTCTTC               GAAGAC(2/6)
BsiEI        1736  CGGCCG               CG[GA][CT]^CG
BsmBI         275  GAGACG               CGTCTC(1/5)
BsmI         1074  GAATGC               GAATGC(1/-1)
BspHI        2328  TCATGA               T^CATGA
BsrFI        1484  ACCGGC               [GA]^CCGG[CT]
BstBI        2366  TTCGAA               TT^CGAA
BstEII       1755  GGTGACC              G^GT[ACGT]ACC
BstPI        1755  GGTGACC              G^GT[ACGT]ACC
CelII        1887  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1484  ACCGGC               [GA]^CCGG[CT]
DrdI         1041  GACCATATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1736  CGGCCG               C^GGCCG
Eam1105I     1603  GACCGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1736  CGGCCG               C^GGCCG
Eco52I       1736  CGGCCG               C^GGCCG
EcoO65I      1755  GGTGACC              G^GT[ACGT]ACC
EcoRI        1064  GAATTC               G^AATTC
EcoT22I       848  ATGCAT               ATGCA^T
FbaI          381  TGATCA               T^GATCA
FspI         1871  TGCGCA               TGC^GCA
HincII       1456  GTCAAC               GT[CT]^[GA]AC
HindII       1456  GTCAAC               GT[CT]^[GA]AC
KasI         1594  GGCGCC               G^GCGCC
KpnI          433  GGTACC               GGTAC^C
KspI         1605  CCGCGG               CCGC^GG
MluNI        1530  TGGCCA               TGG^CCA
MscI         1530  TGGCCA               TGG^CCA
NarI         1594  GGCGCC               GG^CGCC
NsiI          848  ATGCAT               ATGCA^T
NspV         2366  TTCGAA               TT^CGAA
PflFI        2229  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         886  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         880  ATTAAT               AT^TAAT
PstI         1693  CTGCAG               CTGCA^G
PvuII        1813  CAGCTG               CAG^CTG
RcaI         2328  TCATGA               T^CATGA
SacII        1605  CCGCGG               CCGC^GG
SapI         2097  GCTCTTC              GCTCTTC(1/4)
SfoI         1594  GGCGCC               GGC^GCC
SfuI         2366  TTCGAA               TT^CGAA
TfiI           85  GAATC                G^A[AT]TC
Tth111I      2229  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        886  CCAATATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          399  TCTAGA               T^CTAGA
XcmI         1907  CCAAGCACAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AocI         1545  CCTCAGG              CC^T[ACGT]AGG
             2020  CCTGAGG             
Asp700I       856  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1194  GAACTTTTTC          
BanII         298  GAGCCC               G[GA]GC[CT]^C
             1831  GAGCCC              
BbrPI         182  CACGTG               CAC^GTG
             1975  CACGTG              
BsaAI         182  CACGTG               [CT]AC^GT[GA]
             1975  CACGTG              
BsaI         1084  GAGACC               GGTCTC(1/5)
             1509  GGTCTC              
BsrBI        2014  CCGCTC               CCGCTC(-3/-3)
             2217  GAGCGG              
BstXI        1116  CCAAGACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1760  CCAAGGTGGTGG        
BstYI         652  GGATCC               [GA]^GATC[CT]
              801  GGATCT              
Bsu36I       1545  CCTCAGG              CC^T[ACGT]AGG
             2020  CCTGAGG             
DsaI         1605  CCGCGG               C^C[GA][CT]GG
             1826  CCACGG              
EarI         2098  CTCTTC               CTCTTC(1/4)
             2104  CTCTTC              
Eco81I       1545  CCTCAGG              CC^T[ACGT]AGG
             2020  CCTGAGG             
Ksp632I      2098  CTCTTC               CTCTTC(1/4)
             2104  CTCTTC              
MflI          652  GGATCC               [GA]^GATC[CT]
              801  GGATCT              
NlaIII        847  CATG                 CATG^
             2329  CATG                
PmaCI         182  CACGTG               CAC^GTG
             1975  CACGTG              
PmlI          182  CACGTG               CAC^GTG
             1975  CACGTG              
SexAI        1751  ACCTGGT              A^CC[AT]GGT
             2066  ACCTGGT             
SfcI         1570  CTACAG               C^T[GA][CT]AG
             1693  CTGCAG              
SmlI          470  CTCAAG               C^T[CT][GA]AG
             1819  CTCAAG              
SspI           45  AATATT               AAT^ATT
              952  AATATT              
XhoII         652  GGATCC               [GA]^GATC[CT]
              801  GGATCT              
XmnI          856  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1194  GAACTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApoI        AspHI       AvaI        
AvaII       BanI        BbvI        BcnI        BfaI        
BmyI        BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XmaCI       
XmaI        
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