Restriction map for
pF1KB4218
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# NO CUT ENZYMES ( 74 enzymes) #
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AccIII AclI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
BbvCI BfrI BglI BglII BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I ClaI
CpoI DraI DraIII Eco47III EcoNI
EcoRV FseI HindIII HpaI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XhoI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AatII 1327 GACGTC GACGT^C
Acc65I 433 GGTACC G^GTACC
AccI 2062 GTCTAC GT^[AC][GT]AC
AhdI 1603 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 77 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 880 ATTAAT AT^TAAT
AsnI 880 ATTAAT AT^TAAT
Asp718I 433 GGTACC G^GTACC
AspEI 1603 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1871 TGCGCA TGC^GCA
AvrII 153 CCTAGG C^CTAGG
BalI 1530 TGGCCA TGG^CCA
BamHI 652 GGATCC G^GATCC
BbeI 1594 GGCGCC GGCGC^C
BbsI 177 GTCTTC GAAGAC(2/6)
BcgI 2077 GCATTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1106 GTATCC GTATCC(6/5)
BclI 381 TGATCA T^GATCA
BlnI 153 CCTAGG C^CTAGG
BlpI 1887 GCTGAGC GC^T[ACGT]AGC
BpmI 1124 CTGGAG CTGGAG(16/14)
Bpu1102I 1887 GCTGAGC GC^T[ACGT]AGC
BpuAI 177 GTCTTC GAAGAC(2/6)
BsiEI 1736 CGGCCG CG[GA][CT]^CG
BsmBI 275 GAGACG CGTCTC(1/5)
BsmI 1074 GAATGC GAATGC(1/-1)
BspHI 2328 TCATGA T^CATGA
BsrFI 1484 ACCGGC [GA]^CCGG[CT]
BstBI 2366 TTCGAA TT^CGAA
BstEII 1755 GGTGACC G^GT[ACGT]ACC
BstPI 1755 GGTGACC G^GT[ACGT]ACC
CelII 1887 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1484 ACCGGC [GA]^CCGG[CT]
DrdI 1041 GACCATATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1736 CGGCCG C^GGCCG
Eam1105I 1603 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1736 CGGCCG C^GGCCG
Eco52I 1736 CGGCCG C^GGCCG
EcoO65I 1755 GGTGACC G^GT[ACGT]ACC
EcoRI 1064 GAATTC G^AATTC
EcoT22I 848 ATGCAT ATGCA^T
FbaI 381 TGATCA T^GATCA
FspI 1871 TGCGCA TGC^GCA
HincII 1456 GTCAAC GT[CT]^[GA]AC
HindII 1456 GTCAAC GT[CT]^[GA]AC
KasI 1594 GGCGCC G^GCGCC
KpnI 433 GGTACC GGTAC^C
KspI 1605 CCGCGG CCGC^GG
MluNI 1530 TGGCCA TGG^CCA
MscI 1530 TGGCCA TGG^CCA
NarI 1594 GGCGCC GG^CGCC
NsiI 848 ATGCAT ATGCA^T
NspV 2366 TTCGAA TT^CGAA
PflFI 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 886 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 880 ATTAAT AT^TAAT
PstI 1693 CTGCAG CTGCA^G
PvuII 1813 CAGCTG CAG^CTG
RcaI 2328 TCATGA T^CATGA
SacII 1605 CCGCGG CCGC^GG
SapI 2097 GCTCTTC GCTCTTC(1/4)
SfoI 1594 GGCGCC GGC^GCC
SfuI 2366 TTCGAA TT^CGAA
TfiI 85 GAATC G^A[AT]TC
Tth111I 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 886 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 399 TCTAGA T^CTAGA
XcmI 1907 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AocI 1545 CCTCAGG CC^T[ACGT]AGG
2020 CCTGAGG
Asp700I 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1194 GAACTTTTTC
BanII 298 GAGCCC G[GA]GC[CT]^C
1831 GAGCCC
BbrPI 182 CACGTG CAC^GTG
1975 CACGTG
BsaAI 182 CACGTG [CT]AC^GT[GA]
1975 CACGTG
BsaI 1084 GAGACC GGTCTC(1/5)
1509 GGTCTC
BsrBI 2014 CCGCTC CCGCTC(-3/-3)
2217 GAGCGG
BstXI 1116 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1760 CCAAGGTGGTGG
BstYI 652 GGATCC [GA]^GATC[CT]
801 GGATCT
Bsu36I 1545 CCTCAGG CC^T[ACGT]AGG
2020 CCTGAGG
DsaI 1605 CCGCGG C^C[GA][CT]GG
1826 CCACGG
EarI 2098 CTCTTC CTCTTC(1/4)
2104 CTCTTC
Eco81I 1545 CCTCAGG CC^T[ACGT]AGG
2020 CCTGAGG
Ksp632I 2098 CTCTTC CTCTTC(1/4)
2104 CTCTTC
MflI 652 GGATCC [GA]^GATC[CT]
801 GGATCT
NlaIII 847 CATG CATG^
2329 CATG
PmaCI 182 CACGTG CAC^GTG
1975 CACGTG
PmlI 182 CACGTG CAC^GTG
1975 CACGTG
SexAI 1751 ACCTGGT A^CC[AT]GGT
2066 ACCTGGT
SfcI 1570 CTACAG C^T[GA][CT]AG
1693 CTGCAG
SmlI 470 CTCAAG C^T[CT][GA]AG
1819 CTCAAG
SspI 45 AATATT AAT^ATT
952 AATATT
XhoII 652 GGATCC [GA]^GATC[CT]
801 GGATCT
XmnI 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1194 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BbvI BcnI BfaI
BmyI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI