################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI BbvCI BfrI BglI BglII BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstZ17I ClaI CpoI DraI DraIII Eco47III EcoNI EcoRV FseI HindIII HpaI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XhoI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AatII 1327 GACGTC GACGT^C Acc65I 433 GGTACC G^GTACC AccI 2062 GTCTAC GT^[AC][GT]AC AhdI 1603 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 77 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 880 ATTAAT AT^TAAT AsnI 880 ATTAAT AT^TAAT Asp718I 433 GGTACC G^GTACC AspEI 1603 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1871 TGCGCA TGC^GCA AvrII 153 CCTAGG C^CTAGG BalI 1530 TGGCCA TGG^CCA BamHI 652 GGATCC G^GATCC BbeI 1594 GGCGCC GGCGC^C BbsI 177 GTCTTC GAAGAC(2/6) BcgI 2077 GCATTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1106 GTATCC GTATCC(6/5) BclI 381 TGATCA T^GATCA BlnI 153 CCTAGG C^CTAGG BlpI 1887 GCTGAGC GC^T[ACGT]AGC BpmI 1124 CTGGAG CTGGAG(16/14) Bpu1102I 1887 GCTGAGC GC^T[ACGT]AGC BpuAI 177 GTCTTC GAAGAC(2/6) BsiEI 1736 CGGCCG CG[GA][CT]^CG BsmBI 275 GAGACG CGTCTC(1/5) BsmI 1074 GAATGC GAATGC(1/-1) BspHI 2328 TCATGA T^CATGA BsrFI 1484 ACCGGC [GA]^CCGG[CT] BstBI 2366 TTCGAA TT^CGAA BstEII 1755 GGTGACC G^GT[ACGT]ACC BstPI 1755 GGTGACC G^GT[ACGT]ACC CelII 1887 GCTGAGC GC^T[ACGT]AGC Cfr10I 1484 ACCGGC [GA]^CCGG[CT] DrdI 1041 GACCATATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1736 CGGCCG C^GGCCG Eam1105I 1603 GACCGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1736 CGGCCG C^GGCCG Eco52I 1736 CGGCCG C^GGCCG EcoO65I 1755 GGTGACC G^GT[ACGT]ACC EcoRI 1064 GAATTC G^AATTC EcoT22I 848 ATGCAT ATGCA^T FbaI 381 TGATCA T^GATCA FspI 1871 TGCGCA TGC^GCA HincII 1456 GTCAAC GT[CT]^[GA]AC HindII 1456 GTCAAC GT[CT]^[GA]AC KasI 1594 GGCGCC G^GCGCC KpnI 433 GGTACC GGTAC^C KspI 1605 CCGCGG CCGC^GG MluNI 1530 TGGCCA TGG^CCA MscI 1530 TGGCCA TGG^CCA NarI 1594 GGCGCC GG^CGCC NsiI 848 ATGCAT ATGCA^T NspV 2366 TTCGAA TT^CGAA PflFI 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 886 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 880 ATTAAT AT^TAAT PstI 1693 CTGCAG CTGCA^G PvuII 1813 CAGCTG CAG^CTG RcaI 2328 TCATGA T^CATGA SacII 1605 CCGCGG CCGC^GG SapI 2097 GCTCTTC GCTCTTC(1/4) SfoI 1594 GGCGCC GGC^GCC SfuI 2366 TTCGAA TT^CGAA TfiI 85 GAATC G^A[AT]TC Tth111I 2229 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 886 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 399 TCTAGA T^CTAGA XcmI 1907 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AocI 1545 CCTCAGG CC^T[ACGT]AGG 2020 CCTGAGG Asp700I 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1194 GAACTTTTTC BanII 298 GAGCCC G[GA]GC[CT]^C 1831 GAGCCC BbrPI 182 CACGTG CAC^GTG 1975 CACGTG BsaAI 182 CACGTG [CT]AC^GT[GA] 1975 CACGTG BsaI 1084 GAGACC GGTCTC(1/5) 1509 GGTCTC BsrBI 2014 CCGCTC CCGCTC(-3/-3) 2217 GAGCGG BstXI 1116 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1760 CCAAGGTGGTGG BstYI 652 GGATCC [GA]^GATC[CT] 801 GGATCT Bsu36I 1545 CCTCAGG CC^T[ACGT]AGG 2020 CCTGAGG DsaI 1605 CCGCGG C^C[GA][CT]GG 1826 CCACGG EarI 2098 CTCTTC CTCTTC(1/4) 2104 CTCTTC Eco81I 1545 CCTCAGG CC^T[ACGT]AGG 2020 CCTGAGG Ksp632I 2098 CTCTTC CTCTTC(1/4) 2104 CTCTTC MflI 652 GGATCC [GA]^GATC[CT] 801 GGATCT NlaIII 847 CATG CATG^ 2329 CATG PmaCI 182 CACGTG CAC^GTG 1975 CACGTG PmlI 182 CACGTG CAC^GTG 1975 CACGTG SexAI 1751 ACCTGGT A^CC[AT]GGT 2066 ACCTGGT SfcI 1570 CTACAG C^T[GA][CT]AG 1693 CTGCAG SmlI 470 CTCAAG C^T[CT][GA]AG 1819 CTCAAG SspI 45 AATATT AAT^ATT 952 AATATT XhoII 652 GGATCC [GA]^GATC[CT] 801 GGATCT XmnI 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1194 GAACTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI