Restriction map for
pF1KB4239
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspHI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII EaeI EagI
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII KasI
KpnI KspI MluI MluNI MscI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 2519 GACGTC GACGT^C
AccI 538 GTATAC GT^[AC][GT]AC
AccIII 1833 TCCGGA T^CCGGA
AhdI 2386 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1681 ATTAAT AT^TAAT
AsnI 1681 ATTAAT AT^TAAT
Asp700I 1846 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2386 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 2264 GAGCCC G[GA]GC[CT]^C
BciVI 1574 GTATCC GTATCC(6/5)
BcnI 3115 CCGGG CC^[GC]GG
BglI 2925 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 558 AGATCT A^GATCT
BseAI 1833 TCCGGA T^CCGGA
BsgI 927 CTGCAC GTGCAG(16/14)
BsmI 2949 GAATGC GAATGC(1/-1)
Bsp1407I 642 TGTACA T^GTACA
BspEI 1833 TCCGGA T^CCGGA
BspHI 2109 TCATGA T^CATGA
BsrGI 642 TGTACA T^GTACA
Bst1107I 538 GTATAC GTA^TAC
BstXI 2269 CCAGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 538 GTATAC GTA^TAC
DrdI 3484 GACAGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2386 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3063 GATATC GAT^ATC
FauI 2310 GCGGG CCCGC(4/6)
HgaI 2319 GACGC GACGC(5/10)
HpaI 670 GTTAAC GTT^AAC
MroI 1833 TCCGGA T^CCGGA
NciI 3115 CCGGG CC^[GC]GG
NdeI 1588 CATATG CA^TATG
PshBI 1681 ATTAAT AT^TAAT
PstI 1508 CTGCAG CTGCA^G
PvuII 2928 CAGCTG CAG^CTG
RcaI 2109 TCATGA T^CATGA
ScaI 3329 AGTACT AGT^ACT
SexAI 2485 ACCTGGT A^CC[AT]GGT
SspBI 642 TGTACA T^GTACA
XbaI 1788 TCTAGA T^CTAGA
XmnI 1846 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 2319 GACGCC G[GA]^CG[CT]C
2519 GACGTC
AocI 1351 CCTGAGG CC^T[ACGT]AGG
3032 CCTTAGG
BamHI 278 GGATCC G^GATCC
1836 GGATCC
BanI 2933 GGCACC G^G[CT][GA]CC
3547 GGTGCC
BcgI 1335 CGAGTTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1455 CGAAGAAGATGC
BmyI 292 GGGCAC G[AGT]GC[ACT]^C
2264 GAGCCC
BsaBI 2134 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3352 GATTTGCATC
BsaHI 2319 GACGCC G[GA]^CG[CT]C
2519 GACGTC
BsaI 3509 GAGACC GGTCTC(1/5)
3517 GGTCTC
BsaWI 1030 ACCGGA [AT]^CCGG[AT]
1833 TCCGGA
BseRI 989 CTCCTC GAGGAG(10/8)
430 GAGGAG
BsmFI 2808 GGGAC GGGAC(10/14)
3447 GTCCC
Bsp1286I 292 GGGCAC G[AGT]GC[ACT]^C
2264 GAGCCC
Bsu36I 1351 CCTGAGG CC^T[ACGT]AGG
3032 CCTTAGG
Eco81I 1351 CCTGAGG CC^T[ACGT]AGG
3032 CCTTAGG
EcoNI 463 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3427 CCTCTCAAAGG
Hin1I 2319 GACGCC G[GA]^CG[CT]C
2519 GACGTC
MamI 2134 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3352 GATTTGCATC
MfeI 71 CAATTG C^AATTG
443 CAATTG
MspA1I 321 CCGCTG C[AC]G^C[GT]G
2928 CAGCTG
MunI 71 CAATTG C^AATTG
443 CAATTG
PflMI 1123 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2445 CCATGGGGTGG
PshAI 1986 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3511 GACCATGGTC
StuI 471 AGGCCT AGG^CCT
2229 AGGCCT
Van91I 1123 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2445 CCATGGGGTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII