Restriction map for
pF1KB4303
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AviII BbsI
BcgI BciVI BclI BglII BpuAI
BsaBI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI Bst1107I
BstAPI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NheI NotI NruI NsiI
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfaNI SfiI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 1028 TCCGGA T^CCGGA
AfaI 462 GTAC GT^AC
AflII 211 CTTAAG C^TTAAG
AhdI 736 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 796 GGGCCC GGGCC^C
AspEI 736 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1164 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1647 GGATCC G^GATCC
BbeI 1870 GGCGCC GGCGC^C
BbrPI 1309 CACGTG CAC^GTG
BbvCI 1399 GCTGAGG CCTCAGC(-5/-2)
BfrI 211 CTTAAG C^TTAAG
BglI 535 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 507 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC
BseAI 1028 TCCGGA T^CCGGA
BsgI 430 CTGCAC GTGCAG(16/14)
BspEI 1028 TCCGGA T^CCGGA
BspHI 332 TCATGA T^CATGA
BssHII 544 GCGCGC G^CGCGC
BssSI 1807 CTCGTG CACGAG(-5/-1)
BstBI 1102 TTCGAA TT^CGAA
BstEII 154 GGTGACC G^GT[ACGT]ACC
BstPI 154 GGTGACC G^GT[ACGT]ACC
CelII 507 GCTGAGC GC^T[ACGT]AGC
Eam1105I 736 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 354 GAAGAG CTCTTC(1/4)
EcoO65I 154 GGTGACC G^GT[ACGT]ACC
HincII 1517 GTCAAC GT[CT]^[GA]AC
HindII 1517 GTCAAC GT[CT]^[GA]AC
KasI 1870 GGCGCC G^GCGCC
Ksp632I 354 GAAGAG CTCTTC(1/4)
MroI 1028 TCCGGA T^CCGGA
MseI 212 TTAA T^TAA
NaeI 1848 GCCGGC GCC^GGC
NarI 1870 GGCGCC GG^CGCC
NdeI 1660 CATATG CA^TATG
NgoMIV 1848 GCCGGC G^CCGGC
NspV 1102 TTCGAA TT^CGAA
PaeR7I 959 CTCGAG C^TCGAG
PflFI 1164 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 580 GACTC GAGTC(4/5)
PmaCI 1309 CACGTG CAC^GTG
PmlI 1309 CACGTG CAC^GTG
RcaI 332 TCATGA T^CATGA
RsaI 462 GTAC GT^AC
SacI 1423 GAGCTC GAGCT^C
SbfI 756 CCTGCAGG CCTGCA^GG
SexAI 464 ACCTGGT A^CC[AT]GGT
SfoI 1870 GGCGCC GGC^GCC
SfuI 1102 TTCGAA TT^CGAA
SmaI 1637 CCCGGG CCC^GGG
Sse8387I 756 CCTGCAGG CCTGCA^GG
StuI 1755 AGGCCT AGG^CCT
Tru9I 212 TTAA T^TAA
Tth111I 1164 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 959 CTCGAG C^TCGAG
XmaCI 1637 CCCGGG C^CCGGG
XmaI 1637 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 855 GACGCC G[GA]^CG[CT]C
1870 GGCGCC
Alw44I 586 GTGCAC G^TGCAC
604 GTGCAC
AocI 949 CCTGAGG CC^T[ACGT]AGG
1242 CCTCAGG
ApaLI 586 GTGCAC G^TGCAC
604 GTGCAC
AvrII 73 CCTAGG C^CTAGG
1752 CCTAGG
BalI 572 TGGCCA TGG^CCA
648 TGGCCA
BlnI 73 CCTAGG C^CTAGG
1752 CCTAGG
BsaHI 855 GACGCC G[GA]^CG[CT]C
1870 GGCGCC
BsaI 883 GAGACC GGTCTC(1/5)
1066 GGTCTC
BsaWI 1028 TCCGGA [AT]^CCGG[AT]
1095 ACCGGA
BsrBI 523 GAGCGG CCGCTC(-3/-3)
1579 GAGCGG
BstXI 1268 CCACACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1274 CCATGGAACTGG
BstYI 752 AGATCC [GA]^GATC[CT]
1647 GGATCC
Bsu36I 949 CCTGAGG CC^T[ACGT]AGG
1242 CCTCAGG
DrdI 937 GACGAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1249 GACAGTGAGGTC
EagI 533 CGGCCG C^GGCCG
1672 CGGCCG
EclXI 533 CGGCCG C^GGCCG
1672 CGGCCG
Eco52I 533 CGGCCG C^GGCCG
1672 CGGCCG
Eco81I 949 CCTGAGG CC^T[ACGT]AGG
1242 CCTCAGG
Hin1I 855 GACGCC G[GA]^CG[CT]C
1870 GGCGCC
MflI 752 AGATCC [GA]^GATC[CT]
1647 GGATCC
MluNI 572 TGGCCA TGG^CCA
648 TGGCCA
MscI 572 TGGCCA TGG^CCA
648 TGGCCA
NcoI 1157 CCATGG C^CATGG
1274 CCATGG
NspI 347 ACATGC [GA]CATG^[CT]
1120 ACATGC
XhoII 752 AGATCC [GA]^GATC[CT]
1647 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I