Restriction map for
pF1KB4307
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# NO CUT ENZYMES ( 159 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BanI BbeI BbsI BbvCI
BcgI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HgaI HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 535 GACGTC GACGT^C
AcyI 535 GACGTC G[GA]^CG[CT]C
BalI 749 TGGCCA TGG^CCA
BamHI 248 GGATCC G^GATCC
BbrPI 214 CACGTG CAC^GTG
BbvI 795 GCAGC GCAGC(8/12)
BglI 219 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 157 CTGGAG CTGGAG(16/14)
BsaAI 214 CACGTG [CT]AC^GT[GA]
BsaHI 535 GACGTC G[GA]^CG[CT]C
BseRI 814 GAGGAG GAGGAG(10/8)
BsiWI 188 CGTACG C^GTACG
BsiYI 896 CCAAAGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 896 CCAAAGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 533 GAGACG CGTCTC(1/5)
BsmFI 177 GGGAC GGGAC(10/14)
BspMI 640 GCAGGT ACCTGC(4/8)
CfoI 350 GCGC GCG^C
Cfr13I 660 GGCCC G^G[ACGT]CC
EaeI 749 TGGCCA [CT]^GGCC[GA]
EcoRI 891 GAATTC G^AATTC
EcoT14I 104 CCAAGG C^C[AT][AT]GG
FauI 224 GCGGG CCCGC(4/6)
HhaI 350 GCGC GCG^C
Hin1I 535 GACGTC G[GA]^CG[CT]C
HinP1I 350 GCGC G^CGC
HincII 862 GTTGAC GT[CT]^[GA]AC
HindII 862 GTTGAC GT[CT]^[GA]AC
MaeIII 337 GTCAC ^GT[ACGT]AC
MfeI 632 CAATTG C^AATTG
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
MspA1I 222 CAGCGG C[AC]G^C[GT]G
MunI 632 CAATTG C^AATTG
PmaCI 214 CACGTG CAC^GTG
PmlI 214 CACGTG CAC^GTG
PstI 851 CTGCAG CTGCA^G
Sau96I 660 GGCCC G^G[ACGT]CC
SfcI 851 CTGCAG C^T[GA][CT]AG
StyI 104 CCAAGG C^C[AT][AT]GG
TseI 795 GCAGC G^C[AT]GC
Tsp45I 337 GTCAC ^GT[GC]AC
XcmI 308 CCATGAGGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 189 GTAC GT^AC
553 GTAC
AspHI 388 GAGCTC G[AT]GC[AT]^C
682 GAGCTC
BanII 388 GAGCTC G[GA]GC[CT]^C
682 GAGCTC
BciVI 396 GGATAC GTATCC(6/5)
697 GGATAC
BfaI 89 CTAG C^TAG
406 CTAG
BsaJI 30 CCCTGG C^C[ACGT][ACGT]GG
104 CCAAGG
BsiHKAI 388 GAGCTC G[AT]GC[AT]^C
682 GAGCTC
BsrI 174 ACTGG ACTGG(1/-1)
74 CCAGT
BstF5I 753 CATCC GGATG(2/0)
931 CATCC
BstYI 248 GGATCC [GA]^GATC[CT]
377 GGATCT
Cac8I 109 GCTTGC GC[ACGT]^[ACGT]GC
224 GCGGGC
Fnu4HI 210 GCGGC GC^[ACGT]GC
795 GCAGC
FokI 753 CATCC GGATG(9/13)
931 CATCC
ItaI 210 GCGGC GC^[ACGT]GC
795 GCAGC
MaeI 89 CTAG C^TAG
406 CTAG
MaeII 215 ACGT A^CGT
536 ACGT
MflI 248 GGATCC [GA]^GATC[CT]
377 GGATCT
MseI 38 TTAA T^TAA
883 TTAA
MslI 234 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
732 CATCACGATG
MwoI 210 GCGGCACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
219 GCCCAGCGGGC
RsaI 189 GTAC GT^AC
553 GTAC
SacI 388 GAGCTC GAGCT^C
682 GAGCTC
TaqI 10 TCGA T^CGA
759 TCGA
Tru9I 38 TTAA T^TAA
883 TTAA
TthHB8I 10 TCGA T^CGA
759 TCGA
XhoII 248 GGATCC [GA]^GATC[CT]
377 GGATCT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
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AciI AcsI AluI AlwI ApoI
BmyI BsmAI Bsp1286I BssKI BstNI
DdeI DpnII EcoRII HaeIII HinfI
HphI MboI MboII MnlI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI SfaNI TfiI Tsp509I TspRI