Restriction map for
pF1KB4325
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AvrII BalI
BbeI BbrPI BbvCI BciVI BclI
BcnI BfaI BfrI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI HaeII HindIII KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 271 GTAGAC GT^[AC][GT]AC
AccII 582 CGCG CG^CG
AfaI 501 GTAC GT^AC
AocI 556 CCTCAGG CC^T[ACGT]AGG
Asp700I 312 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1081 GAGCTC G[AT]GC[AT]^C
AspI 681 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 824 GGTCC G^G[AT]CC
AviII 374 TGCGCA TGC^GCA
BamHI 704 GGATCC G^GATCC
BbsI 941 GAAGAC GAAGAC(2/6)
BcgI 812 CGAACGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1171 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 741 AGATCT A^GATCT
BpuAI 941 GAAGAC GAAGAC(2/6)
BsaI 982 GAGACC GGTCTC(1/5)
BseRI 1237 GAGGAG GAGGAG(10/8)
BsiEI 647 CGGTCG CG[GA][CT]^CG
BsiHKAI 1081 GAGCTC G[AT]GC[AT]^C
BsmI 377 GCATTC GAATGC(1/-1)
BspHI 1193 TCATGA T^CATGA
BsrDI 438 CATTGC GCAATG(2/0)
BstAPI 45 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 582 CGCG CG^CG
Bsu36I 556 CCTCAGG CC^T[ACGT]AGG
DsaI 605 CCATGG C^C[GA][CT]GG
EaeI 1018 CGGCCA [CT]^GGCC[GA]
EarI 832 CTCTTC CTCTTC(1/4)
Eco81I 556 CCTCAGG CC^T[ACGT]AGG
EcoRI 947 GAATTC G^AATTC
FspI 374 TGCGCA TGC^GCA
HpaI 403 GTTAAC GTT^AAC
Ksp632I 832 CTCTTC CTCTTC(1/4)
MspA1I 1272 CAGCGG C[AC]G^C[GT]G
MvnI 582 CGCG CG^CG
NaeI 573 GCCGGC GCC^GGC
NcoI 605 CCATGG C^CATGG
NgoMIV 573 GCCGGC G^CCGGC
NspI 804 GCATGC [GA]CATG^[CT]
PflFI 681 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1193 TCATGA T^CATGA
RsaI 501 GTAC GT^AC
SacI 1081 GAGCTC GAGCT^C
SapI 831 GCTCTTC GCTCTTC(1/4)
SmlI 388 CTTGAG C^T[CT][GA]AG
SphI 804 GCATGC GCATG^C
Tth111I 681 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 605 CCATGGCGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 312 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 576 GGCGTC G[GA]^CG[CT]C
790 GGCGTC
AlwNI 335 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1150 CAGTCTCTG
BsaHI 576 GGCGTC G[GA]^CG[CT]C
790 GGCGTC
BsgI 511 GTGCAG GTGCAG(16/14)
1183 GTGCAG
BsmFI 694 GGGAC GGGAC(10/14)
516 GTCCC
BspMI 619 GCAGGT ACCTGC(4/8)
1188 GCAGGT
BsrFI 573 GCCGGC [GA]^CCGG[CT]
1016 ACCGGC
CfoI 375 GCGC GCG^C
583 GCGC
Cfr10I 573 GCCGGC [GA]^CCGG[CT]
1016 ACCGGC
EcoT14I 605 CCATGG C^C[AT][AT]GG
660 CCAAGG
FauI 1246 GCGGG CCCGC(4/6)
1274 GCGGG
HapII 574 CCGG C^CGG
1017 CCGG
HgaI 577 GCGTC GACGC(5/10)
791 GCGTC
HhaI 375 GCGC GCG^C
583 GCGC
Hin1I 576 GGCGTC G[GA]^CG[CT]C
790 GGCGTC
HinP1I 375 GCGC G^CGC
583 GCGC
HincII 403 GTTAAC GT[CT]^[GA]AC
916 GTCAAC
HindII 403 GTTAAC GT[CT]^[GA]AC
916 GTCAAC
HpaII 574 CCGG C^CGG
1017 CCGG
MspI 574 CCGG C^CGG
1017 CCGG
StyI 605 CCATGG C^C[AT][AT]GG
660 CCAAGG
TaqI 278 TCGA T^CGA
432 TCGA
TthHB8I 278 TCGA T^CGA
432 TCGA
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanI BanII BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII