Restriction map for
pF1KB4384
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 601 GTCTAC GT^[AC][GT]AC
AccII 68 CGCG CG^CG
AcsI 835 GAATTC [GA]^AATT[CT]
AcyI 1225 GACGCC G[GA]^CG[CT]C
AhdI 867 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 835 GAATTC [GA]^AATT[CT]
Asp700I 1506 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 867 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2040 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1271 CCTCAGC CCTCAGC(-5/-2)
BfaI 850 CTAG C^TAG
BsaAI 946 TACGTG [CT]AC^GT[GA]
BsaHI 1225 GACGCC G[GA]^CG[CT]C
BsaI 958 GAGACC GGTCTC(1/5)
BsaWI 920 ACCGGA [AT]^CCGG[AT]
BsgI 582 CTGCAC GTGCAG(16/14)
BspHI 506 TCATGA T^CATGA
BspLU11I 482 ACATGT A^CATGT
BsrDI 294 CATTGC GCAATG(2/0)
BstAPI 1427 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 68 CGCG CG^CG
Eam1105I 867 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2595 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 835 GAATTC G^AATTC
HaeII 1992 AGCGCC [GA]GCGC^[CT]
HgaI 1225 GACGC GACGC(5/10)
Hin1I 1225 GACGCC G[GA]^CG[CT]C
MaeI 850 CTAG C^TAG
MvnI 68 CGCG CG^CG
NspI 482 ACATGT [GA]CATG^[CT]
PaeR7I 2066 CTCGAG C^TCGAG
PflFI 2040 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 994 CTGCAG CTGCA^G
RcaI 506 TCATGA T^CATGA
SacI 141 GAGCTC GAGCT^C
SexAI 2181 ACCTGGT A^CC[AT]GGT
StuI 158 AGGCCT AGG^CCT
Tsp509I 836 AATT ^AATT
Tth111I 2040 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2066 CTCGAG C^TCGAG
XmnI 1506 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 482 ACATGT A^C[GA][CT]GT
947 ACGTGT
Alw44I 519 GTGCAC G^TGCAC
888 GTGCAC
AocI 805 CCTGAGG CC^T[ACGT]AGG
2226 CCTCAGG
ApaLI 519 GTGCAC G^TGCAC
888 GTGCAC
BanI 71 GGTGCC G^G[CT][GA]CC
1587 GGCACC
BglI 1201 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1995 GCCGTTGGGGC
BlpI 824 GCTCAGC GC^T[ACGT]AGC
1623 GCTAAGC
Bpu1102I 824 GCTCAGC GC^T[ACGT]AGC
1623 GCTAAGC
BsmBI 88 GAGACG CGTCTC(1/5)
1223 GAGACG
BstYI 1900 AGATCC [GA]^GATC[CT]
1923 AGATCC
Bsu36I 805 CCTGAGG CC^T[ACGT]AGG
2226 CCTCAGG
CelII 824 GCTCAGC GC^T[ACGT]AGC
1623 GCTAAGC
Eco81I 805 CCTGAGG CC^T[ACGT]AGG
2226 CCTCAGG
HincII 547 GTCAAC GT[CT]^[GA]AC
1018 GTCAAC
HindII 547 GTCAAC GT[CT]^[GA]AC
1018 GTCAAC
MflI 1900 AGATCC [GA]^GATC[CT]
1923 AGATCC
NaeI 74 GCCGGC GCC^GGC
2471 GCCGGC
NgoMIV 74 GCCGGC G^CCGGC
2471 GCCGGC
SapI 2143 GAAGAGC GCTCTTC(1/4)
1509 GCTCTTC
SmlI 553 CTCAAG C^T[CT][GA]AG
2066 CTCGAG
TaqI 1262 TCGA T^CGA
2067 TCGA
TthHB8I 1262 TCGA T^CGA
2067 TCGA
XhoII 1900 AGATCC [GA]^GATC[CT]
1923 AGATCC
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TfiI TseI Tsp45I TspRI
Van91I XcmI XmaCI XmaI