Restriction map for
pF1KB4387
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BanI BbeI BbrPI BcgI
BclI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I FbaI FseI
FspI HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 51 CGCG CG^CG
AclI 1527 AACGTT AA^CGTT
AcsI 718 GAATTC [GA]^AATT[CT]
AcyI 1381 GGCGTC G[GA]^CG[CT]C
AflIII 563 ACGTGT A^C[GA][CT]GT
AocI 1275 CCTGAGG CC^T[ACGT]AGG
ApaI 4 GGGCCC GGGCC^C
ApoI 718 GAATTC [GA]^AATT[CT]
Asp700I 1672 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 2143 GCTGAGG CCTCAGC(-5/-2)
BciVI 1503 GGATAC GTATCC(6/5)
BglII 1704 AGATCT A^GATCT
BsaAI 562 TACGTG [CT]AC^GT[GA]
BsaHI 1381 GGCGTC G[GA]^CG[CT]C
BssSI 1219 CACGAG CACGAG(-5/-1)
BstUI 51 CGCG CG^CG
BstXI 1562 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1275 CCTGAGG CC^T[ACGT]AGG
Eco81I 1275 CCTGAGG CC^T[ACGT]AGG
EcoNI 2133 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 718 GAATTC G^AATTC
Hin1I 1381 GGCGTC G[GA]^CG[CT]C
KspI 50 CCGCGG CCGC^GG
MvnI 51 CGCG CG^CG
NaeI 755 GCCGGC GCC^GGC
NcoI 747 CCATGG C^CATGG
NgoMIV 755 GCCGGC G^CCGGC
NspI 2102 GCATGC [GA]CATG^[CT]
PpuMI 831 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1527 AACGTT AA^CGTT
SacII 50 CCGCGG CCGC^GG
SphI 2102 GCATGC GCATG^C
TfiI 1319 GAATC G^A[AT]TC
XmnI 1672 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 1254 GTAC GT^AC
1593 GTAC
AflII 994 CTTAAG C^TTAAG
2443 CTTAAG
AlwNI 2032 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
2138 CAGAGGCTG
Aor51HI 145 AGCGCT AGC^GCT
545 AGCGCT
BfaI 896 CTAG C^TAG
1702 CTAG
BfrI 994 CTTAAG C^TTAAG
2443 CTTAAG
BsaWI 1244 ACCGGA [AT]^CCGG[AT]
1781 ACCGGA
BsmBI 1383 CGTCTC CGTCTC(1/5)
181 GAGACG
BspDI 1063 ATCGAT AT^CGAT
1762 ATCGAT
BsrDI 986 GCAATG GCAATG(2/0)
2018 GCAATG
BsrFI 755 GCCGGC [GA]^CCGG[CT]
1214 ACCGGC
Cfr10I 755 GCCGGC [GA]^CCGG[CT]
1214 ACCGGC
ClaI 1063 ATCGAT AT^CGAT
1762 ATCGAT
DrdI 183 GACGTGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2470 GACAACCCCGTC
Eco47III 145 AGCGCT AGC^GCT
545 AGCGCT
EcoRV 1060 GATATC GAT^ATC
1864 GATATC
EcoT22I 114 ATGCAT ATGCA^T
864 ATGCAT
HgaI 1236 GACGC GACGC(5/10)
1382 GCGTC
MaeI 896 CTAG C^TAG
1702 CTAG
NsiI 114 ATGCAT ATGCA^T
864 ATGCAT
PflMI 1032 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2307 CCAAGCTCTGG
RsaI 1254 GTAC GT^AC
1593 GTAC
StuI 419 AGGCCT AGG^CCT
1169 AGGCCT
Tsp509I 20 AATT ^AATT
719 AATT
Van91I 1032 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2307 CCAAGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AluI AlwI AspHI AspI
AvaII BalI BamHI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflFI PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I TspRI Tth111I TthHB8I XcmI
XhoII