Restriction map for
pF1KB4415
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI ApoI AseI
AsnI Asp700I Asp718I AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NheI NlaIII NlaIV NotI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 319 GGCGTC G[GA]^CG[CT]C
AfaI 150 GTAC GT^AC
AhdI 360 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 91 GTGCAC G^TGCAC
AlwI 186 GATCC GGATC(4/5)
ApaLI 91 GTGCAC G^TGCAC
AscI 139 GGCGCGCC GG^CGCGCC
AspEI 360 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 257 TGGCCA TGG^CCA
BanII 277 GAGCTC G[GA]GC[CT]^C
BbvI 252 GCAGC GCAGC(8/12)
BfaI 292 CTAG C^TAG
BsaHI 319 GGCGTC G[GA]^CG[CT]C
BsaI 67 GGTCTC GGTCTC(1/5)
BsaJI 380 CCAAGG C^C[ACGT][ACGT]GG
BseRI 274 GAGGAG GAGGAG(10/8)
BsmAI 68 GTCTC GTCTC(1/5)
BsrDI 306 CATTGC GCAATG(2/0)
BsrI 72 CCAGT ACTGG(1/-1)
BssHII 140 GCGCGC G^CGCGC
BstAPI 93 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 300 GGTGACC G^GT[ACGT]ACC
BstPI 300 GGTGACC G^GT[ACGT]ACC
BstYI 185 AGATCC [GA]^GATC[CT]
Cfr13I 84 GGGCC G^G[ACGT]CC
DpnII 186 GATC ^GATC
Eam1105I 360 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 54 CTCTTC CTCTTC(1/4)
EcoO65I 300 GGTGACC G^GT[ACGT]ACC
EcoT14I 380 CCAAGG C^C[AT][AT]GG
HgaI 320 GCGTC GACGC(5/10)
Hin1I 319 GGCGTC G[GA]^CG[CT]C
Ksp632I 54 CTCTTC CTCTTC(1/4)
MaeI 292 CTAG C^TAG
MaeII 9 ACGT A^CGT
MboI 186 GATC ^GATC
MflI 185 AGATCC [GA]^GATC[CT]
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MspA1I 253 CAGCTG C[AC]G^C[GT]G
NaeI 199 GCCGGC GCC^GGC
NdeII 186 GATC ^GATC
NgoMIV 199 GCCGGC G^CCGGC
NruI 213 TCGCGA TCG^CGA
PvuII 253 CAGCTG CAG^CTG
RsaI 150 GTAC GT^AC
SacI 277 GAGCTC GAGCT^C
SapI 53 GCTCTTC GCTCTTC(1/4)
Sau3AI 186 GATC ^GATC
Sau96I 84 GGGCC G^G[ACGT]CC
SfaNI 233 GCATC GCATC(5/9)
SgrAI 143 CGCCGGTG C[GA]^CCGG[CT]G
StyI 380 CCAAGG C^C[AT][AT]GG
TseI 252 GCAGC G^C[AT]GC
XhoII 185 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AspHI 91 GTGCAC G[AT]GC[AT]^C
277 GAGCTC
BbsI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BcnI 78 CCCGG CC^[GC]GG
297 CCGGG
BmyI 91 GTGCAC G[AGT]GC[ACT]^C
277 GAGCTC
BpmI 70 CTCCAG CTGGAG(16/14)
190 CTGGAG
BpuAI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
277 GAGCTC
Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C
277 GAGCTC
BsrBI 51 CCGCTC CCGCTC(-3/-3)
124 GAGCGG
BsrFI 144 GCCGGT [GA]^CCGG[CT]
199 GCCGGC
Cfr10I 144 GCCGGT [GA]^CCGG[CT]
199 GCCGGC
DdeI 311 CTCAG C^T[ACGT]AG
353 CTAAG
EaeI 197 TGGCCG [CT]^GGCC[GA]
257 TGGCCA
HinfI 228 GACTC G^A[ACGT]TC
366 GAGTC
HphI 300 GGTGA GGTGA(8/7)
369 TCACC
MaeIII 301 GTGAC ^GT[ACGT]AC
368 GTCAC
MnlI 23 GAGG CCTC(7/6)
274 GAGG
NciI 78 CCCGG CC^[GC]GG
297 CCGGG
PleI 228 GACTC GAGTC(4/5)
366 GAGTC
Tsp45I 301 GTGAC ^GT[GC]AC
368 GTCAC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI AluI BsiYI BslI
BssKI BstF5I BstNI BstUI Cac8I
CfoI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MspI MvaI MvnI
MwoI ScrFI