Restriction map for
pF1KB4450
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BpmI BsaBI BsaWI BseAI BseRI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI BssHII Bst1107I BstAPI BstBI
BstUI BstYI BstZ17I Bsu36I ClaI
CpoI DpnII DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FseI HindIII
HinfI HpaI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MseI MunI MvnI NdeI NdeII
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 728 GGCGCC G[GA]^CG[CT]C
AhdI 40 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 864 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 40 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 78 CTCGGG C^[CT]CG[GA]G
AviII 991 TGCGCA TGC^GCA
BbeI 728 GGCGCC GGCGC^C
BfaI 206 CTAG C^TAG
BsaAI 264 CACGTA [CT]AC^GT[GA]
BsaHI 728 GGCGCC G[GA]^CG[CT]C
BsiEI 375 CGACCG CG[GA][CT]^CG
BsoBI 78 CTCGGG C^[CT]CG[GA]G
Bsp1407I 809 TGTACA T^GTACA
BspHI 860 TCATGA T^CATGA
BsrDI 201 CATTGC GCAATG(2/0)
BsrGI 809 TGTACA T^GTACA
BssSI 1096 CACGAG CACGAG(-5/-1)
BstEII 762 GGTGACC G^GT[ACGT]ACC
BstPI 762 GGTGACC G^GT[ACGT]ACC
BstXI 614 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 793 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 40 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 762 GGTGACC G^GT[ACGT]ACC
EcoRV 193 GATATC GAT^ATC
FspI 991 TGCGCA TGC^GCA
Hin1I 728 GGCGCC G[GA]^CG[CT]C
KasI 728 GGCGCC G^GCGCC
MaeI 206 CTAG C^TAG
NaeI 731 GCCGGC GCC^GGC
NarI 728 GGCGCC GG^CGCC
NcoI 557 CCATGG C^CATGG
NgoMIV 731 GCCGGC G^CCGGC
NheI 205 GCTAGC G^CTAGC
PpuMI 292 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 860 TCATGA T^CATGA
SacI 573 GAGCTC GAGCT^C
SexAI 794 ACCTGGT A^CC[AT]GGT
SfcI 1113 CTACAG C^T[GA][CT]AG
SfoI 728 GGCGCC GGC^GCC
SspBI 809 TGTACA T^GTACA
StuI 953 AGGCCT AGG^CCT
XmnI 864 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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ApaI 83 GGGCCC GGGCC^C
1057 GGGCCC
AspHI 145 GTGCTC G[AT]GC[AT]^C
573 GAGCTC
AvaII 152 GGACC G^G[AT]CC
293 GGACC
BbvCI 366 CCTCAGC CCTCAGC(-5/-2)
417 GCTGAGG
BglI 356 GCCTCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
472 GCCCTCCTGGC
BsaI 1123 GAGACC GGTCTC(1/5)
606 GGTCTC
BsiHKAI 145 GTGCTC G[AT]GC[AT]^C
573 GAGCTC
BsmAI 1123 GAGAC GTCTC(1/5)
607 GTCTC
BspLU11I 104 ACATGT A^CATGT
335 ACATGT
BspMI 883 ACCTGC ACCTGC(4/8)
986 GCAGGT
BsrFI 361 ACCGGC [GA]^CCGG[CT]
731 GCCGGC
Cfr10I 361 ACCGGC [GA]^CCGG[CT]
731 GCCGGC
EcoT14I 557 CCATGG C^C[AT][AT]GG
600 CCTTGG
HaeII 182 GGCGCT [GA]GCGC^[CT]
728 GGCGCC
MaeII 265 ACGT A^CGT
896 ACGT
PvuII 188 CAGCTG CAG^CTG
903 CAGCTG
StyI 557 CCATGG C^C[AT][AT]GG
600 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AflIII AluI BalI
BanI BanII BbsI BbvI BcnI
BlpI BmyI Bpu1102I BpuAI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HpaII HphI ItaI Ksp632I MaeIII
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TspRI TthHB8I XcmI