Restriction map for
pF1KB4523
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII Aor51HI ApoI
AscI Asp718I AspI AvrII BalI
BamHI BbeI BbvCI BciVI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstUI
BstXI BstZ17I ClaI CpoI DrdI
DsaI Eco47III EcoNI EcoRI EcoRV
EcoT22I FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 547 GTCTAC GT^[AC][GT]AC
AgeI 884 ACCGGT A^CCGGT
AhdI 1003 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 105 GTGCAC G^TGCAC
AocI 563 CCTCAGG CC^T[ACGT]AGG
ApaLI 105 GTGCAC G^TGCAC
AseI 1168 ATTAAT AT^TAAT
AsnI 1168 ATTAAT AT^TAAT
AspEI 1003 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 132 CACGTG CAC^GTG
BcgI 302 GCAACAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1344 TGATCA T^GATCA
BfaI 935 CTAG C^TAG
BlpI 1488 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1488 GCTCAGC GC^T[ACGT]AGC
BsaAI 132 CACGTG [CT]AC^GT[GA]
BsaWI 884 ACCGGT [AT]^CCGG[AT]
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsrDI 1104 CATTGC GCAATG(2/0)
BssSI 713 CTCGTG CACGAG(-5/-1)
Bsu36I 563 CCTCAGG CC^T[ACGT]AGG
CelII 1488 GCTCAGC GC^T[ACGT]AGC
DraI 1192 TTTAAA TTT^AAA
DraIII 190 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 11 CGGCCG [CT]^GGCC[GA]
EagI 11 CGGCCG C^GGCCG
Eam1105I 1003 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 708 CTCTTC CTCTTC(1/4)
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
Eco81I 563 CCTCAGG CC^T[ACGT]AGG
FbaI 1344 TGATCA T^GATCA
Ksp632I 708 CTCTTC CTCTTC(1/4)
MaeI 935 CTAG C^TAG
MfeI 899 CAATTG C^AATTG
MunI 899 CAATTG C^AATTG
NspI 1040 GCATGT [GA]CATG^[CT]
PinAI 884 ACCGGT A^CCGGT
PmaCI 132 CACGTG CAC^GTG
PmlI 132 CACGTG CAC^GTG
PshBI 1168 ATTAAT AT^TAAT
SacI 255 GAGCTC GAGCT^C
ScaI 1152 AGTACT AGT^ACT
SmaI 77 CCCGGG CCC^GGG
SspI 702 AATATT AAT^ATT
TfiI 1113 GAATC G^A[AT]TC
XmaCI 77 CCCGGG C^CCGGG
XmaI 77 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 888 GTAC GT^AC
1153 GTAC
AflIII 131 ACACGT A^C[GA][CT]GT
133 ACGTGT
ApaI 73 GGGCCC GGGCC^C
366 GGGCCC
Asp700I 237 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
699 GAAAATATTC
AvaI 77 CCCGGG C^[CT]CG[GA]G
1216 CTCGGG
BbsI 1473 GAAGAC GAAGAC(2/6)
1501 GAAGAC
BpuAI 1473 GAAGAC GAAGAC(2/6)
1501 GAAGAC
BseRI 21 CTCCTC GAGGAG(10/8)
1259 GAGGAG
BsgI 952 CTGCAC GTGCAG(16/14)
1495 GTGCAG
BsoBI 77 CCCGGG C^[CT]CG[GA]G
1216 CTCGGG
BspHI 852 TCATGA T^CATGA
1077 TCATGA
BstAPI 107 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
189 GCACCATGTGC
BstEII 331 GGTCACC G^GT[ACGT]ACC
581 GGTTACC
BstPI 331 GGTCACC G^GT[ACGT]ACC
581 GGTTACC
BstYI 495 AGATCC [GA]^GATC[CT]
540 GGATCT
EcoO65I 331 GGTCACC G^GT[ACGT]ACC
581 GGTTACC
EcoT14I 783 CCTTGG C^C[AT][AT]GG
975 CCAAGG
HgaI 531 GACGC GACGC(5/10)
1356 GCGTC
MaeII 133 ACGT A^CGT
1505 ACGT
MflI 495 AGATCC [GA]^GATC[CT]
540 GGATCT
NaeI 55 GCCGGC GCC^GGC
394 GCCGGC
NgoMIV 55 GCCGGC G^CCGGC
394 GCCGGC
PflMI 477 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1370 CCAACTGGTGG
PpuMI 263 AGGACCT [GA]G^G[AT]CC[CT]
862 GGGTCCC
RcaI 852 TCATGA T^CATGA
1077 TCATGA
RsaI 888 GTAC GT^AC
1153 GTAC
SmlI 315 CTTGAG C^T[CT][GA]AG
337 CTCAAG
StyI 783 CCTTGG C^C[AT][AT]GG
975 CCAAGG
Van91I 477 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1370 CCAACTGGTGG
XhoII 495 AGATCC [GA]^GATC[CT]
540 GGATCT
XmnI 237 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
699 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII AviII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI FspI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI