Restriction map for
pF1KB4573
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI Hin1I HpaI
KasI KpnI KspI MaeI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 951 GCGG CCGC(-3/-1)
AhdI 636 GACTATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1855 AGCGCT AGC^GCT
AspEI 636 GACTATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1917 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1926 CCCGAG C^[CT]CG[GA]G
AvaII 968 GGACC G^G[AT]CC
AviII 1190 TGCGCA TGC^GCA
BanI 954 GGCACC G^G[CT][GA]CC
BanII 900 GAGCCC G[GA]GC[CT]^C
BglI 902 GCCCAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1704 AGATCT A^GATCT
BsaI 1309 GAGACC GGTCTC(1/5)
BsaWI 1850 ACCGGA [AT]^CCGG[AT]
BsgI 287 GTGCAG GTGCAG(16/14)
BsmI 1553 GAATGC GAATGC(1/-1)
BsoBI 1926 CCCGAG C^[CT]CG[GA]G
BspHI 516 TCATGA T^CATGA
BsrBI 949 GAGCGG CCGCTC(-3/-3)
BssSI 995 CTCGTG CACGAG(-5/-1)
BstBI 842 TTCGAA TT^CGAA
BstXI 904 CCAACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 636 GACTATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1855 AGCGCT AGC^GCT
EcoRV 1897 GATATC GAT^ATC
FauI 951 GCGGG CCCGC(4/6)
FspI 1190 TGCGCA TGC^GCA
HindIII 442 AAGCTT A^AGCTT
MfeI 1253 CAATTG C^AATTG
MspA1I 75 CAGCTG C[AC]G^C[GT]G
MunI 1253 CAATTG C^AATTG
NspV 842 TTCGAA TT^CGAA
PflFI 1917 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 958 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 967 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 457 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 75 CAGCTG CAG^CTG
RcaI 516 TCATGA T^CATGA
SfcI 525 CTGTAG C^T[GA][CT]AG
SfuI 842 TTCGAA TT^CGAA
SmlI 256 CTCAAG C^T[CT][GA]AG
SphI 1377 GCATGC GCATG^C
Tth111I 1917 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 958 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 803 CCACCCTGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 538 GTAC GT^AC
1230 GTAC
AlwNI 959 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1801 CAGATCCTG
Asp700I 370 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
429 GAAGACGTTC
AspHI 4 GAGCAC G[AT]GC[AT]^C
520 GAGCAC
BcnI 120 CCGGG CC^[GC]GG
1346 CCCGG
BsiHKAI 4 GAGCAC G[AT]GC[AT]^C
520 GAGCAC
BsmAI 1309 GAGAC GTCTC(1/5)
1400 GAGAC
BspMI 1295 ACCTGC ACCTGC(4/8)
1500 GCAGGT
DraII 266 GGGGCCT [GA]G^G[ACGT]CC[CT]
967 GGGACCC
EarI 1743 GAAGAG CTCTTC(1/4)
1780 GAAGAG
EcoO109I 266 GGGGCCT [GA]G^G[ACGT]CC[CT]
967 GGGACCC
HaeII 116 AGCGCC [GA]GCGC^[CT]
1855 AGCGCT
HgaI 355 GCGTC GACGC(5/10)
642 GCGTC
HincII 768 GTCGAC GT[CT]^[GA]AC
1138 GTCGAC
HindII 768 GTCGAC GT[CT]^[GA]AC
1138 GTCGAC
Ksp632I 1743 GAAGAG CTCTTC(1/4)
1780 GAAGAG
MaeII 433 ACGT A^CGT
1921 ACGT
MseI 719 TTAA T^TAA
1438 TTAA
NciI 120 CCGGG CC^[GC]GG
1346 CCCGG
PleI 614 GAGTC GAGTC(4/5)
655 GAGTC
RsaI 538 GTAC GT^AC
1230 GTAC
SalI 768 GTCGAC G^TCGAC
1138 GTCGAC
Tru9I 719 TTAA T^TAA
1438 TTAA
XmnI 370 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
429 GAAGACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccI AcsI AluI AlwI ApoI
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfaNI StuI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII