Restriction map for
pF1KB4575
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HapII HpaII
KasI KspI MfeI MflI MluI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 2165 GGCGTC G[GA]^CG[CT]C
AflIII 540 ACATGT A^C[GA][CT]GT
Alw44I 1894 GTGCAC G^TGCAC
AlwNI 1742 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2567 AGCGCT AGC^GCT
ApaLI 1894 GTGCAC G^TGCAC
AspI 185 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 171 CTCGGG C^[CT]CG[GA]G
BbsI 2536 GAAGAC GAAGAC(2/6)
BlpI 2563 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2563 GCTAAGC GC^T[ACGT]AGC
BpuAI 2536 GAAGAC GAAGAC(2/6)
BsaHI 2165 GGCGTC G[GA]^CG[CT]C
BsmI 1392 GAATGC GAATGC(1/-1)
BsoBI 171 CTCGGG C^[CT]CG[GA]G
BspLU11I 540 ACATGT A^CATGT
BsrDI 1369 CATTGC GCAATG(2/0)
BstXI 20 CCAAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2563 GCTAAGC GC^T[ACGT]AGC
DrdI 745 GACTTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2567 AGCGCT AGC^GCT
EcoRI 1250 GAATTC G^AATTC
EcoT14I 221 CCAAGG C^C[AT][AT]GG
FauI 1517 GCGGG CCCGC(4/6)
Hin1I 2165 GGCGTC G[GA]^CG[CT]C
HpaI 1227 GTTAAC GTT^AAC
PflFI 185 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 202 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2449 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1732 CAGCTG CAG^CTG
ScaI 1425 AGTACT AGT^ACT
SspI 588 AATATT AAT^ATT
StyI 221 CCAAGG C^C[AT][AT]GG
Tth111I 185 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 202 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1308 GGTACC G^GTACC
1923 GGTACC
AccII 1536 CGCG CG^CG
1920 CGCG
Asp718I 1308 GGTACC G^GTACC
1923 GGTACC
BanI 1308 GGTACC G^G[CT][GA]CC
1923 GGTACC
BanII 324 GAGCTC G[GA]GC[CT]^C
1590 GAGCTC
BsaBI 605 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2260 GATAACAATC
BsgI 1599 CTGCAC GTGCAG(16/14)
1781 CTGCAC
BstUI 1536 CGCG CG^CG
1920 CGCG
DraI 994 TTTAAA TTT^AAA
1071 TTTAAA
DraII 795 GGGTCCT [GA]G^G[ACGT]CC[CT]
1938 GGGTCCC
EcoNI 385 CCTATTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1561 CCTGTGAGAGG
EcoO109I 795 GGGTCCT [GA]G^G[ACGT]CC[CT]
1938 GGGTCCC
HaeII 307 AGCGCC [GA]GCGC^[CT]
2567 AGCGCT
HaeIII 383 GGCC GG^CC
1650 GGCC
HgaI 1918 GACGC GACGC(5/10)
2166 GCGTC
HincII 846 GTCAAC GT[CT]^[GA]AC
1227 GTTAAC
HindII 846 GTCAAC GT[CT]^[GA]AC
1227 GTTAAC
HindIII 1663 AAGCTT A^AGCTT
2281 AAGCTT
KpnI 1308 GGTACC GGTAC^C
1923 GGTACC
MamI 605 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2260 GATAACAATC
MspA1I 1515 CAGCGG C[AC]G^C[GT]G
1732 CAGCTG
MvnI 1536 CGCG CG^CG
1920 CGCG
PpuMI 795 GGGTCCT [GA]G^G[AT]CC[CT]
1938 GGGTCCC
SacI 324 GAGCTC GAGCT^C
1590 GAGCTC
SmlI 327 CTCAAG C^T[CT][GA]AG
2571 CTTGAG
StuI 382 AGGCCT AGG^CCT
1649 AGGCCT
XcmI 20 CCAAGAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2196 CCAAGAGAATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AclI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI Psp1406I PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI